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Application of benzamine in preparation of drugs for preventing and treating myocardial ischemia-reperfusion injury or ischemic heart disease

A technology for ischemic heart disease and reperfusion injury, which is applied in the field of biomedicine and can solve the problems that have not yet been reported on the application of benzamine in ischemic heart disease.

Inactive Publication Date: 2020-06-26
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, according to the prior art, benzamine has not been used in the preparation of medicines, and there have been no reports about the application of benzamine in the prevention and treatment of myocardial ischemia-reperfusion injury or ischemic heart disease drugs.

Method used

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  • Application of benzamine in preparation of drugs for preventing and treating myocardial ischemia-reperfusion injury or ischemic heart disease
  • Application of benzamine in preparation of drugs for preventing and treating myocardial ischemia-reperfusion injury or ischemic heart disease
  • Application of benzamine in preparation of drugs for preventing and treating myocardial ischemia-reperfusion injury or ischemic heart disease

Examples

Experimental program
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Embodiment Construction

[0024]The following examples are provided to further illustrate but not limit the present invention.

[0025] 1. Materials and methods:

[0026] 1.1 Rat cardiomyocytes

[0027] Clean-grade newborn male wistar rat cardiomyocytes within 3 days can be obtained after primary culture.

[0028] 1.2 Reagents

[0029] DMEM-H medium (Gibco, U.S.), DMEM-L medium (Gibco, U.S.), fetal bovine serum (Gibco, U.S.), trypsin (Gibco, China), penicillin-streptomycin solution 100X (Gibco, U.S.) , China), PBS (Beijing Zhongshan), Brdu (Gibco, USA), CCK-8 kit (Beyond, China), benzamine (MedChemExpress, China) and metoprolol (Selleck, China).

[0030] 1.3 Instruments

[0031] Biological safety cabinet (BSC-1300ⅡA2, Shanghai Boxun Industrial Co., Ltd.), ultra-clean bench (SW-CJ-2F, Shanghai Boxun Industrial Co., Ltd.), inverted microscope (ECLIPSE Ti-s Nikon, Japan), upright microscope (CX22OLYMPUS , Japan), 0.22um mixed cellulose filter membrane (MILLIPORE, USA), carbon dioxide incubator (Sanyo...

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Abstract

The present invention discloses an application of benzamine in preparation of drugs for preventing and treating myocardial ischemia-reperfusion injury or ischemic heart disease. Through transcriptomedata analysis of peripheral blood of patients with acute coronary syndrome and based on co-expression pathways and a drug gene set enrichment analysis method, a list of drug candidates for preventingand treating the acute coronary syndrome are obtained and comprise several marketed drugs for the acute coronary syndrome, the benzamine having functions of preventing and treating the acute coronarysyndrome is not found in the list, besides, an effect of the benzamine is verified to be comparable to that of metoprolol, a common beta-receptor antagonist cardiovascular disease drug on a hypoxia-reoxygenation cell model that simulates the myocardial ischemia-reperfusion injury and ischemic heart disease, thus the benzamine can be used to prepare the drugs for preventing and treating the myocardial ischemia-reperfusion injury or the ischemic heart disease.

Description

technical field [0001] The invention relates to a new use of medicine, in particular to a new use of benzamine, specifically the application of benzamine in the preparation of medicines for preventing and treating myocardial ischemia-reperfusion injury or ischemic heart disease, and belongs to the field of biomedicine. Background technique [0002] Benzamine (Benzamil) is a specific blocker of epithelial sodium channel (ENaC) (Chalfant, M.L. (1995). "Regulation of epithelial Na+channels from M-1 cortical collectingduct cells". American Journal of Physiology. Renal Physiology .271:f861–f870.doi:10.1152 / ajprenal.1996.271.4.f861.), can inhibit the reabsorption of sodium ions in the renal tubules and collecting ducts, and can promote Decreased concentration of sodium ions and water in the body. It is also a sodium-calcium exchange blocker (Gomez-Sanchez, E.P.; Gomez-Sanchez C.E. (September 1995). "Effect of central infusion of benzamil on Dahl S rat hypertension". Am JPhysiol.2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4965A61K45/06A61K31/4365A61K31/138A61K31/40A61K31/277A61K31/4422A61K31/401A61K31/21A61P9/10A61P9/00
CPCA61K31/138A61K31/21A61K31/277A61K31/40A61K31/401A61K31/4365A61K31/4422A61K31/4965A61K45/06A61P9/00A61P9/10A61K2300/00
Inventor 贾志龙何昆仑王卫东石金龙杨晶贾倩
Owner GENERAL HOSPITAL OF PLA
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