Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of bostrycin

A spore and coiling technology, applied in the new application field of cirrhoids, can solve the problems such as unclear mechanism of action of tongue cancer cells

Inactive Publication Date: 2020-06-26
WUHAN POLYTECHNIC UNIVERSITY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the inhibitory effect of bostrycin on tongue squamous cell carcinoma has not been reported, and the mechanism of inhibition of tongue cancer cells is still unclear.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of bostrycin
  • Application of bostrycin
  • Application of bostrycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Cell Proliferation Experiment

[0030] MTT experiment: SCC9 cells and SCC25 cells were inoculated in 96-well plates at 5000 cells / well, and 12 hours later, different concentrations of cipherocine were added. After 24 hours, 48 ​​hours and 72 hours of treatment, 20 μL of 5 mg / mL MTT solution was added to each well, and cultured. After 4 hours, the supernatant was discarded, and 150 μL of DMSO was added to each well to dissolve the formazan. After the formazan was completely dissolved, the absorbance was measured at 490 nm with a microplate reader.

[0031] Colony formation experiment: inoculate cells at 1000 cells / dish in 35mm culture dish, add cipherocine after 12h, change medium after 24h of treatment, discard supernatant after 7 days of culture, fix with methanol / acetic acid fixative solution at room temperature for 30min, 2 % crystal violet staining for 1h. After washing with PBS for 3 times, pictures were taken and counted by Image J software.

[0032]...

Embodiment 2

[0035] Example 2 Cell Cycle Experiment

[0036] Inoculate SCC9 cells and SCC25 cells on 35mm dishes, culture them to 70% confluence, add ciprosporin, collect cells after culturing for 24 hours, fix with pre-cooled 70% ethanol at -20°C overnight, collect cells, wash once with PBS, add 80 μL of propidium iodide (PI) with a concentration of 100 μg / mL, 0.25% Triton-100, and RNase with a final concentration of 20 μg / mL were incubated at 4° C. in the dark for 30 min, and detected by flow cytometry.

[0037] Results: Cyclosporine increased the G2 / M phase arrest of tongue cancer cells SCC9 and SCC25.

[0038] Such as figure 2 A-2D, where, figure 2 A and figure 2 B against SCC9 cells, figure 2 C and figure 2 D against SCC25 cells. After the tongue cancer cells were treated with ciclosporin for 24 hours, the cirrhoids increased the sub-G1 phase of SCC9 cells by 2.0%, decreased the G0 / G1 phase by 13.2%, and decreased the S phase by 4.6% at 2 μg / mL. phase increased by 1.9%; ...

Embodiment 3

[0039] Example 3 Cell Apoptosis Kit Double Staining Detection Cell Apoptosis Experiment

[0040] SCC9 cells and SCC25 cells were inoculated in 24-well plates, cultured to 70% confluence and added with ciprosporine, and after 24 hours of culture, the cells were collected and stained with Annexin V-Alexa Fluor 488 / PI apoptosis kit (Yeasen Biotech, Shanghai, China), apoptosis was detected by flow cytometry.

[0041] For AO / EB experiments, SCC9 cells and SCC25 cells were inoculated in 12-well plates, cultured overnight to 70% confluence, and crizosporine was added, and cultured for 24h and 48h, respectively. Discard the culture medium, take 2mg / mL AO and EB, take 1μL AO and 1μL EB respectively, add 1mL PBS to dilute, mix well, prepare and use now, add 100μL staining working solution to each well, place at room temperature for 2-5min to take pictures with a fluorescence microscope.

[0042] Inoculate SCC9 cells and SCC25 cells in 12-well plates, culture overnight to 70% confluen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
control rateaaaaaaaaaa
Login to View More

Abstract

The invention relates to application of bostrycin. Researches of the invention show that the bostrycin is capable remarkably inhibiting proliferation and migration of tongue cancer cells, retarding the cycles of the cell in a G2 / M stage and causing potential change of a mitochondrial membrane, so as to cause apoptosis. According to the bostrycin, the potential change of the mitochondrial membraneand increase of BAX expression are induced, and cell apoptosis is induced by inducing a mitochondria apoptosis pathway, so that growth of the tongue cancer cells is inhibited.

Description

technical field [0001] The invention belongs to the field of biomedicine, and more specifically relates to a new application of cirrhoids. Background technique [0002] Oral cancer is one of the most common malignant tumors in the head and neck, and its incidence is increasing year by year worldwide. Among them, tongue cancer is the most typical and common type of oral cancer. 90% of oral cancer is squamous cell carcinoma, and tongue squamous cell carcinoma is as high as 50% to 60%, ranking first. Studies have shown that there are about 10990 new cases of tongue squamous cell carcinoma in the world every year. Surgery, chemotherapy, radiotherapy and other treatment methods have been used to treat squamous cell carcinoma of the tongue. Although the level of medical technology has made great progress, the prognosis of patients with squamous cell carcinoma of the tongue is still unsatisfactory, and the 5-year survival rate is only About 50%, local or regional recurrence and c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P35/00
CPCA61K31/122A61P35/00
Inventor 张军林刘志国赖晓晶揭晶岳硕豪熊超刘娜
Owner WUHAN POLYTECHNIC UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com