ADSCs and EPCs stem cell system capable of promoting recovery of microcirculation blood supply of graft

Abstract

The invention discloses an ADSCs and EPCs stem cell system capable of promoting recovery of microcirculation blood supply of a graft. The ADSCs and EPCs stem cell system capable of promoting recoveryof microcirculation blood supply of the graft is composed of adipose-derived stem cells (ADSCs) derived from adipose tissue and endothelial progenitor cells (EPCs) derived from peripheral blood. The ADSCs are extracted by adopting an enzymolysis method for culture; and the peripheral blood is collected, after heparin is used for anticoagulation and methylcellulose is used for sedimentation of redblood cells, gradient centrifugation is conducted by adopting a lymphocyte separation medium, and the EPCs are collected and purified by adopting an adherence method. The ADSCs and the EPCs which arecultured separately are subjected to co-culture according to a cell ratio of the ADSCs to the EPCs of 1:1 and a total cell concentration of 20000 / cm<2>. According to the ADSCs and EPCs stem cell system capable of promoting recovery of microcirculation blood supply of the graft, nutrition supply of the graft is improved, transplanted adipose tissue survival and adipose cell differentiation are promoted, the transplantation efficiency is improved, and a treatment cycle is shortened. On the other hand, all the cells are extracted from the body of a patient, injury is minor, and pain is little, sothat the ADSCs and EPCs stem cell system capable of promoting recovery of microcirculation blood supply of the graft is liable to be accepted by the patient.

Description

technical field [0001] The invention relates to clinical treatment technology, in particular to an ADSCs and EPCs stem cell system that can promote the restoration of graft microcirculation blood supply. Background technique [0002] Tissue transplantation is a common clinical treatment in plastic surgery. At present, skin flap transplantation can be used for burn plastic surgery and scar resection, and autologous fat transplantation can be used for full face filling, breast augmentation and other therapeutic fields. The graft vascularization and restoration of microcirculatory blood supply directly affect the survival rate of tissue transplantation. The most important step for early tissue graft survival is to vascularize the graft tissue as soon as possible to improve blood supply to prevent graft necrosis, liquefaction, and absorption. It is one of the keys to effectively promote the survival of transplanted tissues while promoting vascularization after transplantation a...

AI Technical Summary

Problems solved by technology

[0005] The most common type of tissue transplantation is fat transplantation, and the main method in clinical work is simple fat transplantation, but this method takes a long time to restore the blood supply of microcirculation, and it is difficult to vascularize fat tissue transplantation, which easily leads to necrosis, liquefaction and absorption of fat tissue. , Difficulty in survival, need for repeated treatment, etc.

We found in our work that most patients are resistant to repeated treatments, which increases the patient’s psychological burden and physical pain. In addition, the low survival rate of fat grafting will affect the doctor’s judgment on fat filling and utilization efficiency. to achieve the desired effect

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