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Atomizing agent for repairing damaged lung tissue and electronic cigarette smoke oil

A technology for tissue damage and aerosolization, which is applied in aerosol delivery, tobacco, tobacco processing, etc., can solve problems such as lung injury, achieve the effect of reducing harmful components, reducing injuries, and simple preparation methods

Pending Publication Date: 2020-06-19
刘思维 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with traditional cigarettes, although harmful components can be reduced, the main components of e-liquid still retain harmful substances such as nicotine and propylene glycol, and long-term inhalation will still cause certain damage to the lungs of smokers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The aerosol for repairing lung tissue damage provided in this example consists of bronchial basal layer cell supernatant and physiological saline.

[0031] Its preparation method is as follows:

[0032] The isolated active bronchial brushing tissue was taken for later use.

[0033] Take the tissue digestion solution and stop solution for later use; 99v% of the tissue digestion solution is DMEM / F12, and the rest are 1-20ng / mL DNase, 0.1-4mg / mL protease and 10-200ng / mL trypsin; stop solution 90v% is DMEM, 10v% is FBS.

[0034] The above-mentioned brushed tissue is digested with the tissue digestion solution, and the digested tissue is terminated with the stop solution, and then the cells are collected.

[0035] Take the medium for use, in which 225mL DMEM, 225mL F12, 20-70mL FBS, 0.2-2mM L-glutamine, 1-14ng / mL insulin, 0.1-1ng / mL epidermal growth factor, 5-30ug / mL in the medium Adenine, 2-20ug / mL hydrocortisone.

[0036] Take part of the digested cells, resuspend them...

Embodiment 2

[0040] An electronic cigarette liquid is composed of the following components by weight: 1 g of bronchial basal layer cell supernatant, 35 g of glycerin, 30 g of propylene glycol, 1 g of nicotine salt and 5 g of fragrance.

[0041] Heat glycerin to 75°C, add propylene glycol, and stir at 75°C, 300rpm / min for 5min to obtain mixture 1;

[0042] When the above-mentioned mixture 1 was cooled down to 45°C, nicotine salt and spices were added, and stirred at 45°C and 300rpm / min for 10min to obtain mixture 2;

[0043] When the above mixture 2 was cooled down to 37°C, the supernatant of bronchial basal layer cells prepared in Example 1 was added, and stirred at 37°C and 300 rpm / min for 5 minutes to obtain mixture 3;

[0044] The above mixture 3 was placed in a vacuum bottle and stored at 37°C for 12 hours;

[0045] Transfer the e-cigarette oil from the vacuum bottle to a room temperature environment and store it for more than 10 days before it can be used.

Embodiment 3

[0047] An electronic cigarette liquid is composed of the following components by weight: 6 g of bronchial basal layer cell supernatant, 60 g of glycerin, 60 g of propylene glycol, 5 g of nicotine salt and 10 g of fragrance.

[0048] Heat glycerin to 75°C, add propylene glycol, and stir at 75°C, 200rpm / min for 10min to obtain mixture 1;

[0049] When the above-mentioned mixture 1 was cooled down to 45°C, nicotine salt and spices were added, and stirred at 40°C and 300 rpm / min for 5 minutes to obtain mixture 2;

[0050] When the above mixture 2 was cooled to 37°C, the bronchial basal layer cell supernatant prepared in Example 1 was added, and stirred at 30°C and 300 rpm / min for 5 minutes to obtain mixture 3;

[0051] The above mixture 3 was placed in a vacuum bottle and stored at 10°C for 12 hours;

[0052] Transfer the e-cigarette oil from the vacuum bottle to a room temperature environment and store it for more than 10 days before it can be used.

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Abstract

The invention discloses an atomizing agent for repairing damaged lung tissue and electronic cigarette smoke oil in the field of biotechnology application. The active ingredient of the atomizing agentfor repairing damaged lung tissue is a supernatant of cells of the basal layer of the bronchia. The electronic cigarette smoke oil contains the following components in parts by weight: 1-6 parts of the supernatant of the cells of the basal layer of the bronchia, 35-60 parts of glycerin, 30-60 parts of propylene glycol, 1-5 parts of nicotine base and / or nicotine salt and 5-10 parts of spice. The atomizing agent for repairing damaged lung tissue has a simple composition, the active ingredient, the supernatant of cells of the basal layer of the bronchia, of the atomizing agent has an obvious lungrepairing function, the damaged lung tissue can be repaired, and the lung function can be restored; and through the electronic cigarette smoke oil, not only can harmful components of existing cigarette oil products be reduced, but also improvement and repair of the functions of the human lung can be promoted during application of electronic cigarettes through a novel biotechnology method, so thatbenefits of electronic cigarette oil products are increased greatly.

Description

technical field [0001] The invention relates to the application field of biotechnology, in particular to an atomizer for repairing lung tissue damage and an electronic cigarette liquid. Background technique [0002] The lung is the respiratory organ of the human body. When exposed to some foreign or internal harmful substances, a large number of cells in the lung will die and induce an inflammatory response, resulting in large-scale lung tissue damage. Such massive damage often triggers various lung diseases such as chronic obstructive pulmonary disease, bronchiectasis and pulmonary fibrosis, among others. Smoking and passive smoking are among the causes of lung tissue damage. Harmful substances such as carbon monoxide, nicotine, and aldehydes contained in cigarette smoke are closely related to lung tissue damage. [0003] E-cigarette is an electronic device that delivers atomized e-liquid to the respiratory system by means of electronic heating. At present, the e-liquid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/42A61K9/12A61P11/00A24B15/167
CPCA61K35/42A61K9/12A61P11/00A24B15/167
Inventor 刘思维左为马奕阳张婷
Owner 刘思维
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