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Application of mesenchymal stem cell derived extracellular vesicles to cerebral ischemia-reperfusion injury

A technology of cerebral ischemia reperfusion and cells, which is applied in the direction of medical preparations containing active ingredients, cardiovascular system diseases, unknown raw materials, etc., can solve problems such as incompletely clear mechanisms, and achieve the reduction of neurological deficits and tissue damage , the effect of reducing apoptosis

Active Publication Date: 2020-06-19
SHANDONG UNIV QILU HOSPITAL
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Problems solved by technology

However, the inventors found that in the ischemia-reperfusion model, the mechanisms by which MSCs-EVs exert neuroprotective effects through AMPK and JAK2 / STAT3 / NF-κB signaling pathways are not fully understood

Method used

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  • Application of mesenchymal stem cell derived extracellular vesicles to cerebral ischemia-reperfusion injury
  • Application of mesenchymal stem cell derived extracellular vesicles to cerebral ischemia-reperfusion injury
  • Application of mesenchymal stem cell derived extracellular vesicles to cerebral ischemia-reperfusion injury

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Experimental program
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Embodiment

[0076] 1. Experimental animals

[0077] Adult healthy male SPF grade SD rats weighing 280-320 g were selected for this study, and the experimental animals were purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd. (permission number: SCXK(LU)2019-0003). They were raised in the SPF grade animal breeding room of Shandong University Animal Center, with temperature (24±1°C), humidity of 45%-55%, and natural light / dark cycle (≈12h-12h). All rats had free access to food and water. Experiments were started after 7 days of adaptive feeding. All animal experimental procedures were performed in accordance with the guiding principles of the Chinese Care and Use Act and were approved by the Animal Ethics Committee of Shandong University Health Sciences.

[0078] 2. Establishment of MCAO model in rats

[0079] The modified Longa method was used to establish the rat MCAO (cerebral ischemia-reperfusion) model. The rats were fasted for 6 h before the operation to reduce comp...

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Abstract

The invention provides application of mesenchymal stem cell derived extracellular vesicles to cerebral ischemia-reperfusion injury. Research of the invention finds and verifies that extracellular vesicles (MSCs-EVs) derived from mesenchymal stem cells are capable of treating disordered brain functions and taking a neuroprotective effect into play through neurogenesis and angiogenesis after stroke.Research results show that within 24 hours and 48 hours after MCAO (middle cerebral artery occlusion), MSCs-EVs are capable of remarkably reliving neurologic impairment of rats, reducing the volume of cerebral infarction and brain water contents, relieving pathological injury of brain tissue cortexes, weakening cortex neuronal apoptosis, remarkably increasing p-AMPK (protein kinase), and reducingp-JAK2, p-STAT3 and p-NF-kB. Due to the application, essential support is provided for potential treatment strategies of MSCs-EVs as MCAO treatment.

Description

technical field [0001] The invention belongs to the technical field of crude drugs and medicine, and in particular relates to the application of extracellular vesicles derived from mesenchymal stem cells in cerebral ischemia-reperfusion injury. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Cerebrovascular disease is the third leading cause of death in the world and one of the leading causes of death among Chinese residents. Its morbidity and mortality are increasing year by year, bringing heavy burden to society and economy. Ischemic stroke accounts for 75-80% of all strokes, and the incidence rate is significantly higher than that of hemorrhagic stroke. The pathologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P9/10
CPCA61K35/28A61P9/10
Inventor 李刚王贞韩敏薛皓曹颖初锡丽辛丹清李婷婷柯鸿飞
Owner SHANDONG UNIV QILU HOSPITAL
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