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Tumor angiogenesis model and its preparation method and application

A tumor angiogenesis and model technology, applied in the field of biological three-dimensional printing, can solve problems such as defects, uneven distribution of tumor cells, and contact inhibition

Active Publication Date: 2022-04-15
THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] In the above cited documents, co-cultivation is used to build a three-dimensional model, which is likely to cause contact inhibition of the two types of cells during the growth process, and does not conform to the distribution of tumor cells in the body.
The distribution of tumor cells on the material is uneven, and the tumor cells themselves are in a two-dimensional microenvironment, which is not conducive to the biological function of tumor cells
[0012] In summary, although the prior art discloses both the tumor angiogenesis model constructed by 2D culture and the tumor angiogenesis model constructed by 3D culture, the tumor angiogenesis model in the prior art still exists. defect

Method used

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  • Tumor angiogenesis model and its preparation method and application
  • Tumor angiogenesis model and its preparation method and application
  • Tumor angiogenesis model and its preparation method and application

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preparation example Construction

[0123] In the , the present invention also provides a method for preparing the tumor angiogenesis model according to the , the tumor angiogenesis model includes hydrogel microfibers, and the hydrogel microfibers Be the structure of columnar body, wherein, the preparation method of described hydrogel microfiber comprises the steps:

[0124] Raw material preparation step: preparing a first hydrogel solution and a second hydrogel solution respectively, wherein the first hydrogel solution contains a first hydrogel material, and the first hydrogel material is loaded with tumors cells; the second hydrogel solution contains a second hydrogel material;

[0125] Printing step: using bioprinting technology to print the first hydrogel solution as a shell structure, and print the second hydrogel solution as a core structure, the shell structure is in contact with the core structure, and the shell structure is located at the radially outside the core structure.

[0126] Wherein, the firs...

Embodiment 1

[0186] Human glioma cells U87 and human umbilical vein endothelial cells HUVEC were cultured in complete medium, and the two cells were collected for transfection experiments when they were in the logarithmic growth phase. Prepare 3 × 10 with complete medium 4 / mL of cell suspension, according to the growth rate of each cell, add 1×10 4 cells, cultured at 37°C for 24 hours, and when the confluence of the cells reached 30%, 2 μL of 1×10 8 TU / mL virus infection solution mediating RFP or GFP gene, wherein, adding virus infection solution mediating RFP gene to human glioma cell U87; adding virus mediating GFP gene to human umbilical vein endothelial cells HUVEC The infection solution was cultured at 37°C for 16 hours, and the culture medium was replaced to continue the culture; human glioma cell U87-RFP cell suspension and human umbilical vein endothelial cell HUVEC-GFP cell suspension were respectively obtained.

[0187] After 72 hours of infection, observe the infection effici...

Embodiment 2

[0193] Human glioma cell U118 and human brain microvascular endothelial cell HBMEC were respectively cultured in complete medium, and were collected for transfection experiments when the two cells were in the logarithmic growth phase. Prepare 4 × 10 with complete medium 4 / mL of cell suspension, according to the growth rate of each cell, add 2×10 4 cells, cultured at 37°C for 24 hours, and when the confluence of the cells reached 20%, 3 μL of 1×10 8TU / mL virus infection solution mediating RFP or GFP gene, wherein, adding virus infection solution mediating RFP gene to human glioma cell U118; adding virus mediating GFP gene to human brain microvascular endothelial cells HBMEC The infection solution was cultured at 37°C for 14 hours, and the culture medium was replaced to continue the culture; human glioma cell U118-RFP cell suspension and human brain microvascular endothelial cell HBMEC-GFP cell suspension were respectively obtained.

[0194] After 72 hours of infection, obser...

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Abstract

The invention provides a tumor angiogenesis model and its preparation method and application. The tumor angiogenesis model includes hydrogel microfibers, the hydrogel microfibers have a columnar structure, the hydrogel microfibers have a shell structure and a core structure in contact, and the shell structure is located on the radially outward of the core structure; wherein the shell structure is derived from a first hydrogel material loaded with tumor cells; the core structure is derived from a second hydrogel material . The tumor angiogenesis model of the invention can simulate the three-dimensional structure of the microenvironment of the tumor tissue in the body, and is beneficial to the paracrine and autocrine functions of tumor cells and endothelial cells. Moreover, the two kinds of cells do not produce contact inhibition during the growth process, and conform to the distribution law of tumor cells in vivo.

Description

technical field [0001] The invention belongs to the field of biological three-dimensional printing or the field of tumor models, and in particular relates to a tumor angiogenesis model and a preparation method thereof. Background technique [0002] Traditional methods for studying tumor angiogenesis in vitro are mainly to culture tumor cells and / or endothelial cells in 2D culture dishes, while in vivo studies mainly rely on animal models. [0003] First of all, 2D culture has the advantages of easy operation and controllable culture conditions, but the cells in 2D culture grow in a single layer, lack of cell-cell and cell-extracellular matrix interactions, and cannot simulate the microenvironment of tumor tissue in vivo. The three-dimensional structure of cells, and the interaction between cells and the three-dimensional microenvironment are crucial for tumor growth and angiogenesis. Second, 2D cultured cells have sufficient oxygen and nutrients, which is inconsistent with ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09C12N5/071C12N5/10
CPCC12N5/0693C12N5/0691C07K14/43595C12N2533/74C12N2533/54C12N2533/72C12N2533/80C12N2510/00
Inventor 王宣之龙小燕
Owner THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE
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