Interdigital arrangement conductive nanotube sensing device for cardiac muscle cell multi-element activity detection

Abstract

The invention discloses a sensing device for cardiac muscle cell multi-element activity detection. The device comprises a PCB and multiple groups of interdigital electrodes, the plurality of groups ofinterdigital electrodes are distributed in a central symmetry manner. Each interdigital electrode comprises a bus and an interdigital electrode array, a plurality of interdigital electrode pairs is vertically distributed at one end of each bus to form the interdigital electrode array, and interdigital electrode arrays of every two buses are oppositely arranged in a staggered mode to form the interdigital electrode. The other end of each bus is connected with a bonding pad on PCB. A culture cavity for culturing cells is fixed on the surface of the interdigital electrode. The interdigital electrode is composed of a conductive nano hollow tube deposited on the PET porous membrane. According to the invention, the platinum hollow nanotube array is used as a three-dimensional biological interface; the prepared sensor has the advantages of good biocompatibility, large specific surface area and better coupling with cells, so that the prepared sensor can greatly improve the detection sensitivity of various signals while realizing the multi-element activity synchronous detection of electrophysiology, growth and mechanical pulsation signals and metabolic signals of myocardial cells.

Description

Technical field [0001] The invention relates to an interdigitated conductive nanotube sensor, in particular to a sensor device capable of detecting multiple activities of myocardial cells. Background technique [0002] At present, the detection indicators for cardiomyocyte activity include myocardial electrical signal, electrical impedance, and biochemical markers, which reflect the electrophysiology, growth, and metabolism of cardiomyocytes. However, there is usually a single problem with these parameter detection and sensing devices, and it is impossible to study the internal connection of the coordinated changes between the various parameters of the cardiomyocytes under the action of internal and external factors. At the same time, the sensor devices usually used for cardiomyocyte detection are often micron-scale planar electrodes, which have poor coupling with cell membranes, which affects the sensitivity of detecting cardiomyocyte electrophysiological signals, growth and mec...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing myocardial sensing device cannot synchronously detect cell electrophysiological signals, growth and mechanical beating signals, and metabolic signals, and at the same time, the micron-scale planar electrode has poor coupling effect with cells and cannot penetrate the cell membrane. Disadvantages, developed a sensing device based on interdigitated conductive nanotubes for simultaneous detection of multiple activities of cardiomyocyte electrophysiology, growth and mechanical beating signals, and metabolic signals

Images