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The kasp primer set associated with resistance to scab in wheat and its application

A technology related to wheat head blight and resistance, applied in the field of crop breeding and molecular biology, can solve problems such as difficulty in using disease-resistant sites, poor agronomic traits, large genetic and physical distances, etc.

Active Publication Date: 2022-07-29
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology

[0005] So far, more than 100 scab resistance QTLs have been reported and linked molecular markers have been reported (S. Liu, M.D. Hall, C. A. Griffey, A. L. McKendry, Meta-analysis of QTL associated with fusarium headblight resistance in wheat, Crop Sci. 49(2009) 955-1968), but there are generally two problems when using molecular markers linked to these loci: (1) The agronomic traits of the source of resistance are poor, and the resistance QTL loci are often linked to poor agronomic traits. Traits, disease resistance loci are difficult to use
(2) These markers are mainly SSR markers reported earlier, the genetic distance and physical distance between markers are large, and the prediction effect is poor. New molecular markers need to be developed (W.C.Zhou, F.L.Kolb, G.H.Bai, L.L.Dromie, L.K.Boze, N.J.Smith,Validation of a major QTL forscab resistance with SSR markers and use of marker-assisted selection inwheat,Plant Breed.122(2003)40-46); Most molecular markers are not closely linked with disease resistance genes or QTL, within a certain group Developed markers that are less predictive within other genetic background populations or within natural populations
Fhb1 is also widely used in breeding scab resistant varieties (Y.Hao, A.Rasheed, Z.Zhu, B.B.H.Wulff, Z.He, Harnessing Wheat Fhb1 for Fusarium Resistance, Trends in Plant Science (2019), https : / / doi.org / 10.1016 / j.tplants.2019.10.006), but Fhb1 can only explain 20-50% of the phenotypic variation of wheat head blight resistance, and wheat varieties with only genes cannot reach Sufficient disease resistance requires accumulation with other resistance loci or genes (G.Bai, Z.Q.Su, J.Cai, Wheat resistance to Fusariumhead blight, Can.J.Plant Pathol.40(2018)336-346)

Method used

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  • The kasp primer set associated with resistance to scab in wheat and its application
  • The kasp primer set associated with resistance to scab in wheat and its application
  • The kasp primer set associated with resistance to scab in wheat and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1, Qfhb.3AL17928 molecular marker development:

[0034] In this example, Yangmai 158 and Zhengmai 9023 were used as the female parent and male parent (reserved seeds in the laboratory of Jiangsu Academy of Agricultural Sciences), and a total of 231 recombinant inbred lines F were obtained by single-grain transmission. 5:7 As material, it was planted in greenhouses for two consecutive years in 2016–2017 and 2017–2018.

[0035] Wheat seeds were first sown in 96-hole plug trays. After the seeds absorbed water, the plug trays were placed in a vernalization room (6°C) for vernalization for 28 days, and then transferred to the greenhouse and planted in flower pots. Each pot was planted with 8 plants, and each line was planted. 2 pots, arranged randomly. In the first month after planting, set the daytime temperature to 17±2°C and the night temperature to 13±2°C, then set the daytime temperature to 22±5°C and the night temperature to 19±2°C until maturity. During the ...

Embodiment 2

[0044] Embodiment 2, Qfhb.3AL17928 is verified in the recombinant inbred line:

[0045] 231 recombinant inbred lines F for the recombinant inbred line population in Example 1 5:7 The wheat samples were detected by KASP marker amplification using Qfhb.3AL17928 according to the method of Example 1, and the detection results were as follows: figure 2 shown. figure 2 Among them, the blank control N, the disease-resistant SNP allelic variation g, and the susceptible SNP allelic variation a were well-typed, and the coincidence rate with the GBS sequencing SNP allelic variation in Example 1 was 100%. It indicated that the KASP marker was successfully developed, and Qfhb.3AL17928 could be further applied to prediction of resistance to Fusarium head blight in wheat samples.

Embodiment 3

[0046] Embodiment 3, Qfhb.3AL17928 is verified in natural population:

[0047] In order to verify the effectiveness of Qfhb.3AL17928, 74 cultivars or high-generation lines were selected from the wheat areas in the middle and lower reaches of the Yangtze River, and were inoculated and identified by 3 points for 2 consecutive years. The inoculation method is similar to Example 1, selects 10 wheat ears with the F0609 conidia suspension of F. graminearum strong pathogenicity race, is bagged and moisturizing 72h after the inoculation, and investigates the diseased smallness of each wheat ear 14 days after the inoculation. The number of spikes, the number of diseased spikelets of each variety (line) was calculated as the average of 10 wheat spikes, as shown in Table 1.

[0048] Table 1 Qfhb.3AL17928 SNP allelic variation and number of diseased spikelets in 74 wheat varieties (lines)

[0049]

[0050]

[0051] Genomic DNA extraction was the same as in Example 1. Using the KAS...

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Abstract

The invention discloses a KASP primer set related to resistance to wheat scab and its application, belonging to the field of crop breeding; the primer set comprises nucleotide sequences such as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO. The specific primer shown in ID NO.3; the KASP primer set can be used for genotype identification of the molecular marker Qfhb.3AL17928. The experiment shows that the resistance of wheat samples with SNP locus genotype g is statistically better than that of Fusarium head blight. The genotype is a wheat sample; the KSAP primer set can be used for molecular marker-assisted selection breeding to improve the breeding efficiency of resistance to scab.

Description

technical field [0001] The invention relates to the field of crop breeding and molecular biology, in particular to a molecular marker closely linked with a wheat scab resistance QTL and its application. Background technique [0002] Wheat scab is a worldwide wheat disease caused by Fusarium graminearum Schwabe, which often occurs in warm humid and semi-humid wheat production areas. Diseases seriously affect the yield and quality of wheat (Cheng Shunhe, Guo Wenshan, Wang Longjun, Jiang Dong, Ma Hongxiang, Zhang Boqiao, Wheat in Southern China, Nanjing: Jiangsu Science and Technology Press, 2012, pp 281–282). More seriously, excessive wheat grain toxins caused by susceptible diseases directly endanger human and animal health (Shi Jianrong, Liu Xin, Qiu Jianbo, Ji Fang, Xu Jianhong, Dong Fei, Yin Xianchao, Ran Junjian, Fusarium toxin deoxynivalensis in wheat). Research progress of enol pollution status and prevention and control, China Agricultural Science, 47(2014) 3641–3654)...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2600/156C12Q2563/107
Inventor 张平平郭程瑾宋桂成姜朋马鸿翔
Owner JIANGSU ACAD OF AGRI SCI
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