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A method for cultivating broad-spectrum, persistent panicle blast-resistant rice breeding materials

A technology of resistance breeding and panicle blast, applied in the field of rice molecular breeding, to slow down the mutation rate, improve the efficiency of breeding selection, and ensure the effect of broad spectrum

Active Publication Date: 2017-05-17
JIANGSU LIXIAHE REGION AGRI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

, but there is no research yet to report whether the aggregation of these two genes has the effect of panicle blast resistance

Method used

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  • A method for cultivating broad-spectrum, persistent panicle blast-resistant rice breeding materials
  • A method for cultivating broad-spectrum, persistent panicle blast-resistant rice breeding materials
  • A method for cultivating broad-spectrum, persistent panicle blast-resistant rice breeding materials

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Embodiment 1

[0026] (1) Using the donor parent Gumei No. 4 carrying the resistant gene Pigm (Yiwen Deng, et al. Genetic characterization and fine mapping of the blast resistance locus Pigm(t) tightly linked to Pi2 and Pi9 in a broad-spectrum resistant Chinese variety. Theoretical and Applied Genetics,2006,113(4):705-713) and the donor parent K3 carrying the resistance gene Pi54 (Yamada M, et al.Proposal of a new method for differentiating races of Pyricularia oryzae Cavara in Japan.Ann Phytopathol Soc Jpn,1976,42(2):216-219) were crossed with the recurrent parent Yangdao 6 to obtain F 1 , respectively with F 1 - Pigm, F 1 -Pi54 said.

[0027] (2) planting F 1 -Pigm and F 1 -Pi54 seeds, the recurrent parent Yangdao No. 6 was backcrossed at flowering stage to obtain 1 generation of backcross, respectively with BC 1 f 1 -Pigm, BC 1 f 1 -Pi54 said.

[0028] (3) planting BC 1 f 1 -Pigm, BC 1 f 1 -Pi54 seeds, each BC was extracted at the seedling stage 1 f 1 Isolate the DNA of ind...

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Abstract

The invention discloses a method for cultivating broad-spectrum and long-lasting panicle blast resistance breeding materials, which belongs to the technical field of rice molecular breeding. The present invention selects conventional rice or hybrid rice parents with large promotion area in production and excellent comprehensive traits as recurrent parents, through continuous backcrossing and polymerization hybridization, combined with molecular marker-assisted selection, the broad-spectrum disease resistance genes Pigm and Pi54 are realized in the Aggregation in breeding material. At the same time, by selecting representative strains for artificial inoculation identification of blast blast, identification of natural induction of rice blast recurrent areas, and evaluation of basic agronomic traits during the whole growth period, improved strains with excellent panicle blast resistance were obtained. The agronomic traits of the improved strains bred by the method of the invention are similar or identical to those of recurrent parents after planting, but the broad-spectrum and persistent resistance to panicle blast are significantly improved.

Description

technical field [0001] The invention belongs to the technical field of rice molecular breeding, and relates to a method for cultivating broad-spectrum and long-lasting panicle blast-resistant rice breeding materials. Background technique [0002] Rice blast caused by the fungus Magnaporthe oryzae is one of the most serious diseases of rice, which seriously threatens the sustained high and stable yield of rice. According to statistics, the global annual output loss due to rice blast is 10%-30%, the direct economic loss is about 5 billion US dollars, and the lost grain is enough to feed 60 million people. In my country, rice blast occurs to varying degrees every year. Especially in recent years, it has continued to occur in the southwestern, middle and lower reaches of the Yangtze River, and Northeast. kg, which has brought huge hidden dangers to the safe production of rice in my country. Practice has proved that using disease-resistant genes to breed resistant varieties is o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 李爱宏吴云雨戴正元余玲潘存红肖宁李育红张小祥周长海黄年生赵步洪季红娟刘晓静蒋敏
Owner JIANGSU LIXIAHE REGION AGRI RES INST
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