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KASP primer group related to wheat scab resistance and application of KASP primer group

A technology related to wheat head blight and resistance, applied in the field of crop breeding and molecular biology, can solve problems such as difficulty in using disease-resistant sites, poor agronomic traits, large genetic and physical distances, etc.

Active Publication Date: 2020-06-12
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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AI Technical Summary

Problems solved by technology

[0005] So far, more than 100 scab resistance QTLs have been reported and linked molecular markers have been reported (S. Liu, M.D. Hall, C. A. Griffey, A. L. McKendry, Meta-analysis of QTL associated with fusarium headblight resistance in wheat, Crop Sci. 49(2009) 955-1968), but there are generally two problems when using molecular markers linked to these loci: (1) The agronomic traits of the source of resistance are poor, and the resistance QTL loci are often linked to poor agronomic traits. Traits, disease resistance loci are difficult to use
(2) These markers are mainly SSR markers reported earlier, the genetic distance and physical distance between markers are large, and the prediction effect is poor. It is necessary to develop new molecular markers (W.C.Zhou, F.L.Kolb, G.H.Bai, L.L.Dromie, L.K. Boze, N.J.Smith,Validation of a major QTL forscab resistance with SSR markers and use of marker-assisted selection inwheat,Plant Breed.122(2003)40-46); Most molecular markers are not closely linked with disease resistance genes or QTL, within a certain group Developed markers that are less predictive within other genetic background populations or within natural populations
Fhb1 is also widely used in breeding scab resistant varieties (Y.Hao, A. Rasheed, Z.Zhu, B.B.H.Wulff, Z.He, Harnessing Wheat Fhb1 for Fusarium Resistance, Trends in Plant Science (2019), https : / / doi.org / 10.1016 / j.tplants.2019.10.006), but Fhb1 can only explain 20-50% of the phenotypic variation of wheat head blight resistance, and wheat varieties with only genes cannot reach Sufficient disease resistance requires accumulation with other resistance loci or genes (G.Bai, Z.Q.Su, J.Cai, Wheat resistance to Fusariumhead blight, Can.J.Plant Pathol.40(2018)336-346)

Method used

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  • KASP primer group related to wheat scab resistance and application of KASP primer group
  • KASP primer group related to wheat scab resistance and application of KASP primer group
  • KASP primer group related to wheat scab resistance and application of KASP primer group

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1, Qfhb.3AL17928 molecular marker development:

[0034] In this example, Yangmai 158 and Zhengmai 9023 were used as the female parent and male parent (retained seeds in the laboratory of Jiangsu Academy of Agricultural Sciences), respectively, and a total of 231 recombinant inbred lines F were obtained by single-grain transmission. 5:7 As materials, it was planted in the greenhouse for two consecutive years in 2016–2017 and 2017–2018.

[0035] First sow wheat seeds in 96-hole plug trays. After the seeds absorb water, put the plug trays into the vernalization chamber (6°C) for vernalization for 28 days, then transfer them to the greenhouse and plant them in flower pots. 8 plants are planted in each pot, and each line is planted. 2 pots, randomly arranged. In the first month after planting, set the daytime temperature to 17±2°C and the night temperature to 13±2°C, then set the daytime temperature to 22±5°C and the night temperature to 19±2°C until maturity. Duri...

Embodiment 2

[0044] Example 2, verification of Qfhb.3AL17928 in recombinant inbred lines:

[0045] 231 parts of recombinant inbred line F 5:7 According to the method of Example 1, the wheat sample was carried out by using Qfhb.3AL17928 to carry out KASP marker amplification detection, and the detection results were as follows: figure 2 shown. In Figure 2, the blank control N, the allelic variation g of the disease-resistant SNP, and the allelic variation a of the susceptible SNP are well-typed, and the coincidence rate with the SNP allelic variation in GBS sequencing in Example 1 is 100%. It indicated that KASP markers were successfully developed, and Qfhb.3AL17928 could be further applied to the prediction of scab resistance in wheat samples.

Embodiment 3

[0046] Example 3, verification of Qfhb.3AL17928 in natural populations:

[0047] In order to verify the effectiveness of Qfhb.3AL17928, 74 cultivars or high-generation lines were selected from the middle and lower reaches of the Yangtze River wheat region, and were inoculated with head blight at 3 points for 2 consecutive years. The inoculation method is similar to that of Example 1. The conidia suspension of the strong pathogenicity race F0609 of Fusarium graminearum is selected to inoculate 10 ears of wheat. After inoculation, it is bagged and kept moist for 72 hours. After the inoculation, the disease degree of each ear of wheat is investigated 14 days after the inoculation. Ear number, calculate the sick spikelet number of each variety (line) as the average value of 10 wheat ears, as shown in Table 1.

[0048] Table 1 Allelic variation of Qfhb.3AL17928 SNP and number of diseased spikelets in 74 wheat cultivars (lines)

[0049]

[0050]

[0051] Genomic DNA extractio...

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Abstract

The invention discloses a KASP primer group related to wheat scab resistance and application of the KASP primer group, and belongs to the field of crop breeding. The primer group comprises specific primers of which the nucleotide sequences are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3 in sequence; the KASP primer group can perform genotype identification on a molecular marker Qfhb.3AL17928, and experiments show that the wheat scab resistance of a wheat sample with the SNP locus genotype g is statistically superior to that of a wheat sample with the genotype a; the KSAP primer group canbe used for molecular marker-assisted selective breeding, and the gibberellic disease resistance breeding efficiency is improved.

Description

technical field [0001] The invention relates to the field of crop breeding and molecular biology, in particular to a molecular marker closely linked with wheat scab resistance QTL and its application. Background technique [0002] Wheat head blight is a worldwide wheat disease caused by Fusarium graminearum Schwabe. It often occurs in warm humid and semi-humid wheat production areas. The disease seriously affects the yield and quality of wheat (Cheng Shunhe, Guo Wenshan, Wang Longjun, Jiang Dong, Ma Hongxiang, Zhang Boqiao, South China Wheat, Nanjing: Jiangsu Science and Technology Press, 2012, pp 281–282). What's more serious is that the excessive wheat grain toxins caused by infection directly endanger the health of humans and animals (Shi Jianrong, Liu Xin, Qiu Jianbo, Ji Fang, Xu Jianhong, Dong Fei, Yin Xianchao, Ran Junjian, Fusarium toxin deoxynivalensis in wheat Current status of enol pollution and research progress in prevention and control, Chinese Agricultural Sci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2600/156C12Q2563/107
Inventor 张平平郭程瑾宋桂成姜朋马鸿翔
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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