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Tissue culture method of cymbidium hybridum

A technology for tissue culture and Cymbidium, applied in the field of artificial propagation and cultivation of plants, can solve the problems of complicated operation, low reproduction coefficient and high required cost, and achieve the effects of simplifying production procedures, improving culture efficiency and increasing reproduction speed.

Pending Publication Date: 2020-06-12
无锡向山兰园科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cymbidium is mostly a hybrid variety. Seed propagation cannot maintain its variety characteristics and has a low seed setting rate. Therefore, traditional cultivation relies on division propagation. However, this method has a long cycle, low reproduction coefficient, and slow propagation speed, which is far from being commercialized. Therefore, at present, the propagation and cultivation of Cymbidium at home and abroad mostly focus on its tissue culture.
[0003] However, the prior art still has the following deficiencies in the tissue culture technology of Cymbidium: ① using the stem tip, stem segment or root as the explant, the acquisition process is relatively complicated and technically difficult, and it will cause or cause damage to the mother plant. Large or small losses; ②The protocorm approach makes the induced starting material go through the process from dedifferentiation to redifferentiation, the mutation rate is high, and it is necessary to separate the protocorm and clustered buds before rooting and strong seedlings, which is cumbersome to operate; ③The method of directly forming seedlings by clustering buds without going through the protocorm route has low induction rate and multiplication rate, and the effect of subsequent rooting and strong seedlings is not good, and the medium components used for induction, multiplication and rooting are generally complicated Variety, high preparation process requirements, and correspondingly high cost

Method used

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  • Tissue culture method of cymbidium hybridum
  • Tissue culture method of cymbidium hybridum
  • Tissue culture method of cymbidium hybridum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Preparation and pretreatment of explants:

[0031] Select the peduncle of Cymbidium cymbidium that has bloomed and grown well, cut it from the base, and divide it into small sections of 3 cm; first treat the peduncle at 35°C for 5 minutes, then at 45°C for 5 minutes, and cool it naturally. Soak in a mixed solution of monoglyceride citrate (10g / L) and astaxanthin (5g / L) for 30min, take it out and blot dry with filter paper; rinse the pedicels fully under running water, put in 0.1% chlorine Soak in mercuric solution for 30 minutes, then rinse with sterile water for 5 times, and finally dry the water with sterile filter paper to obtain explants for later use.

[0032] (2) Induction and proliferation of clustered buds:

[0033] Inoculate the explants into a special medium to induce clustered buds. The formulation of the medium is: MS medium, sodium nitrophenolate 1mg / L, Cymbidium leaf extract 100mL / L, agar 8g / L; The culture temperature is 28°C, the light intensity is ...

Embodiment 2

[0039] (1) Preparation and pretreatment of explants:

[0040]Select the peduncle of Cymbidium cymbidium that has bloomed and grown well, cut it from the base, and divide it into small sections of 2 cm; first treat the peduncle at 30°C for 10 minutes, then at 42°C for 10 minutes, and cool it naturally. Soak in a mixed solution of monoglyceride citrate (5g / L) and astaxanthin (10g / L) for 60min, take it out and blot dry with filter paper; rinse the pedicels fully under running water, put in 0.1% chlorine Soak in mercuric solution for 30 minutes, then rinse with sterile water for 5 times, and finally dry the water with sterile filter paper to obtain explants for later use.

[0041] (2) Induction and proliferation of clustered buds:

[0042] Inoculate the explants into a special medium to induce clustered buds. The formulation of the medium is: MS medium, sodium nitrophenolate 1mg / L, Cymbidium leaf extract 100mL / L, agar 8g / L; The culture temperature is 25°C, the light intensity is...

Embodiment 3

[0048] (1) Preparation and pretreatment of explants:

[0049] Select the peduncle of Cymbidium cymbidium that has bloomed and grown well, cut it from the base, and divide it into small sections of 5 cm; first treat the peduncle at 38°C for 2 minutes, then at 50°C for 1 minute, and cool it naturally. Soak in a mixed solution of monoglyceride citrate (15g / L) and astaxanthin (2g / L) for 10min, take it out and blot dry with filter paper; rinse the pedicels fully under running water, put in 0.1% chlorine Soak in mercuric solution for 30 minutes, then rinse with sterile water for 5 times, and finally dry the water with sterile filter paper to obtain explants for later use.

[0050] (2) Induction and proliferation of clustered buds:

[0051] Inoculate the explants into a special medium to induce clustered buds. The formulation of the medium is: MS medium, sodium nitrophenolate 1mg / L, Cymbidium leaf extract 100mL / L, agar 8g / L; The culture temperature is 30°C, the light intensity is 2...

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Abstract

The invention discloses a tissue culture method of cymbidium hybridum. Particularly, bloomed pedicels are selected as explants, and the treatment of stepwise temperature increase and soaking treatmentof a mixed solution are carried out before disinfection is carried out, so that the brown change rate of the explants is reduced to less than 5%; the pre-treated explants are subjected to cluster budinducing and multiplication in a special medium, cluster buds are directly induced to form seedlings at a time, the multiplication speed is greatly increased, and the maternal excellent traits can bemaintained, so that mutant seedlings are not produced; and the multiplied cluster buds are inoculated into a liquid medium to for rooting and strengthening of seedlings, the two mediums used in the cultivation process have simple and similar components, a leaf extract solution of the cymbidium hybridum and a specific plant growth regulator are added in the mediums correspondingly, so that the induction rate and rooting rate can reach as high as 100%, and the multiplication coefficient is as high as 10.69. The tissue culture method is simple and feasible, the production difficulty and production cost are reduced, the cultivation efficiency and quality are improved, and the market prospect and popularization value are great.

Description

technical field [0001] The invention relates to a tissue culture method of cymbidium, belonging to the technical field of artificial propagation and cultivation of plants. Background technique [0002] Cymbidium hybridium (Cymbidium hybridium) is a perennial herbaceous plant of the genus Orchid, native to southwest China. It is some large-flowered varieties with strong epiphytes in the Orchidaceae and an artificial hybrid mainly obtained from these original species. kind. Its flowering period is long (2-3 months) and it opens during the winter and Spring Festival. and China Orchid), is also an important cut orchid species. Cymbidium is mostly a hybrid variety. Seed propagation cannot maintain its variety characteristics and has a low seed setting rate. Therefore, traditional cultivation relies on division propagation. However, this method has a long cycle, low reproduction coefficient, and slow propagation speed, which is far from being commercialized. Therefore, the prop...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 古屋进
Owner 无锡向山兰园科技有限公司
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