Cyclamen plant callus and bud culture medium and culture method

Abstract

The invention provides a culture medium for inducing cyclamen leaf to form callus and bud and a culture method. Formation of cyclamen callus and bud is highly-efficiently induced by a two-step method, and explant browning is effectively inhibited. The culture method comprises the following steps: before acquisition of explant leaves, cyclamen plant undergoes shading treatment for 24-26 h, and afterwards, in vitro low-temperature (20-22 DEG C) darkness culture is carried out so as to effectively solve the browning problem; then, callus is induced through low-temperature darkness culture, culture temperature is raised to 25-28 DEG C after 30-40 days, and light culture is carried out to induce bud formation; and finally, root induction is conducted after bud has been formed for 15-20 days. The technical system can effectively inhibit browning, has high propagation coefficient, can preserve original traits and heterosis of F1 generation, and provides key technical support for factory production of cyclamen seedlings.

Description

technical field [0001] The invention belongs to the field of plant asexual propagation, in particular to a culture medium and a culture method for inducing cyclamen leaves to form calluses and buds. Background technique [0002] Cyclamenpersicum Mill, also known as Yipinguan, Rabbit Ear Flower, and Radish Begonia, is a perennial bulbous herbaceous flower of Primulaceae Cyclamen. Long, it is one of the top potted flowers in Europe, Japan and other countries, and the market price is expensive. The suitable temperature for the growth of cyclamen is 18-20°C. Cyclamen reproduction methods are divided into sexual reproduction and asexual reproduction. Commodity cyclamen are mostly hybrid generation F 1 , it is difficult to preserve F by seed propagation 1 The original traits and heterosis of the generation, while the seed germination rate is low, the more difficult it is to produce the seeds of varieties with higher ornamental value, which has become the main factor restrictin...

AI Technical Summary

Problems solved by technology

Domestic high-quality varieties of cyclamen are extremely scarce, and foreign original species are not only expensive, but also because most of them are F 1 Generation, continued breeding will lead to separation of traits, and long-term introduction of foreign seeds will cause high production costs, so it is urgent to establish a breeding system for fine domestic cyclamen varieties