Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of ursolic acid magnetic molecularly imprinted nano-microsphere and the extraction method of ursolic acid in Cornus officinalis

A magnetic molecular imprinting, nano-microsphere technology, applied in chemical instruments and methods, separation by moving adsorbents, other chemical processes, etc., can solve the problems of difficult extraction of ursolic acid and large adsorption capacity, and achieve the extraction process. Simple, fast, highly selective and specific effects

Active Publication Date: 2022-04-22
NANYANG NORMAL UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a ursolic acid magnetic molecule imprinted nano-microsphere, the ursolic acid magnetic molecular imprinted nano-microsphere has a large adsorption capacity for ursolic acid, and can realize rapid separation of target objects in complex components by manipulating a magnetic field. Avoid repeated centrifugation, filtration and other procedures to solve the problem of difficulty in extracting ursolic acid in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of ursolic acid magnetic molecularly imprinted nano-microsphere and the extraction method of ursolic acid in Cornus officinalis
  • A kind of ursolic acid magnetic molecularly imprinted nano-microsphere and the extraction method of ursolic acid in Cornus officinalis
  • A kind of ursolic acid magnetic molecularly imprinted nano-microsphere and the extraction method of ursolic acid in Cornus officinalis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 The preparation method of ursolic acid magnetic molecularly imprinted nano-microspheres of the present invention

[0032] 1.1 Preparation of carboxyl-modified magnetic nanospheres: Add ferric chloride hexahydrate with a mass concentration of 1.5-5% and sodium citrate dihydrate with a mass concentration of 0.5-2% to 100mL of ethylene glycol in turn, and react at 200°C for 12 After ~24 hours, the product was washed with deionized water and separated by magnetic field to obtain carboxyl-modified magnetic nanospheres. The particle size of the magnetic molecular imprinted nano microspheres prepared by the present invention is about 200nm.

[0033]1.2 Preparation of ursolic acid magnetic molecularly imprinted nanospheres: using ursolic acid as a template molecule, adding functional monomers, magnetic nanospheres, cross-linking agents and initiators to the solution in sequence, stirring mechanically at room temperature, and polymerizing After 12 hours, the reacti...

Embodiment 2

[0034] Example 2 Preparation of ursolic acid magnetic molecularly imprinted nanospheres

[0035] 2.1 (1) Add 3.25% ferric chloride hexahydrate and 1.0% sodium citrate dihydrate to 100 mL of ethylene glycol in turn, react at 200°C for 12 hours, wash the product with deionized water, and separate it with a magnetic field to obtain Carboxyl-modified magnetic nanospheres.

[0036] (2) Using ursolic acid as a template molecule, functional monomers, magnetic nanospheres, cross-linking agents and initiators were sequentially added to the solution, mechanically stirred at room temperature, and the reaction product was magnetically separated after 12 hours of polymerization, and deionized After washing with water and ethanol as reactants, ursolic acid magnetic molecularly imprinted nanospheres were prepared, and non-imprinted magnetic microspheres were prepared as a control;

[0037] Wherein, the consumption of ursolic acid is 10mg; Functional monomer is triaminopropyltriethoxysilane ...

Embodiment 3

[0045] The adsorption kinetic curve of magnetic molecularly imprinted nanospheres (MNP@MIP) prepared by the present invention for separating ursolic acid from Cornus officinalis to ursolic acid is determined as follows:

[0046] The ursolic acid magnetic molecularly imprinted nanosphere (0.2g) that 2.1 in embodiment 2 prepares is placed in 200mL concentration and is 100mg L -1 In the ursolic acid standard solution, the microspheres were collected by magnetic separation at 0, 5min, 10min, 15min, 20min, 25min, 30min, 35min, 40min, and 45min respectively, and the ultraviolet absorption value was measured at 210nm, and determined according to the ursolic acid standard curve The concentration of remaining ursolic acid in the solution was calculated, and the adsorption amount of molecularly imprinted microspheres to ursolic acid was calculated at different times. The specific test results are as follows: figure 2 shown.

[0047] Depend on figure 2 It can be seen that the adsorpt...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
adsorption capacityaaaaaaaaaa
adsorption capacityaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of biological separation and natural product extraction, and in particular relates to an ursolic acid magnetic molecular imprinted nano-microsphere and a method for extracting ursolic acid from dogwood. The preparation method of described ursolic acid magnetic molecule imprinted nano microsphere comprises the following steps: (1) taking 1.5~5wt% ferric chloride hexahydrate, 0.5~2wt% sodium citrate dihydrate as raw materials, and using ethylene glycol using a hydrothermal synthesis method as a solvent to prepare carboxyl-modified magnetic nano-microspheres; (2) using raw materials including the magnetic nano-microspheres to prepare ursolic acid magnetic molecularly imprinted nano-microspheres. The ursolic acid magnetic molecularly imprinted nano-microspheres proposed by the present invention have a large adsorption capacity for ursolic acid, and can realize rapid separation of target objects in complex components by manipulating the magnetic field, which can effectively avoid repeated centrifugation, filtration and other procedures, and can effectively The problem of difficulty in extracting ursolic acid from dogwood is solved.

Description

technical field [0001] The invention belongs to the field of biological separation and natural product extraction, and in particular relates to an ursolic acid magnetic molecular imprinted nano-microsphere and a method for extracting ursolic acid from dogwood. Background technique [0002] Cornus Officinalis Sieb.et Zucc., as a kind of Chinese herbal medicine, has the effects of invigorating the stomach, nourishing the liver and kidney, curing anemia, lumbago, and weakness of the nerves and heart. The main production areas in my country are western Henan, Zhejiang and other places. Ursolic acid (UA) is a pentacyclic triterpenoid compound in the main active ingredient of Cornus officinalis, which has physiological functions such as anti-inflammatory, anti-tumor, immune regulation, and antitussive. At present, the methods used to extract ursolic acid from plants mainly include leaching method, Soxhlet extraction method, ultrasonic method, resin adsorption method, enzymatic hy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/26C08G77/26C08K9/04C08K7/18B01J20/26B01J20/28B01J20/30B01D15/02C07J63/00C08L83/08
CPCC08J9/26C08G77/26C08K9/04C08K7/18B01J20/268B01J20/28009B01J20/28016B01D15/02C07J63/008C08K2201/01C08K2201/003C08J2383/08C08J2201/0422
Inventor 张鑫李彦松汤波
Owner NANYANG NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com