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Ursolic acid magnetic molecularly imprinted nano-microsphere and method for extracting ursolic acid from dogwood

A technology of magnetic molecular imprinting and nano-microspheres, applied in chemical instruments and methods, separation with mobile adsorbents, alkali metal oxides/hydroxides, etc., can solve the problem of difficult extraction of ursolic acid, large adsorption capacity, etc. problem, to achieve the effect of simple and fast extraction process, simplified separation and purification steps, saving time and economic cost

Active Publication Date: 2020-06-09
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a ursolic acid magnetic molecule imprinted nano-microsphere, the ursolic acid magnetic molecular imprinted nano-microsphere has a large adsorption capacity for ursolic acid, and can realize rapid separation of target objects in complex components by manipulating a magnetic field. Avoid repeated centrifugation, filtration and other procedures to solve the problem of difficulty in extracting ursolic acid in the prior art

Method used

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  • Ursolic acid magnetic molecularly imprinted nano-microsphere and method for extracting ursolic acid from dogwood
  • Ursolic acid magnetic molecularly imprinted nano-microsphere and method for extracting ursolic acid from dogwood
  • Ursolic acid magnetic molecularly imprinted nano-microsphere and method for extracting ursolic acid from dogwood

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Effect test

Embodiment 1

[0031] Embodiment 1 The preparation method of ursolic acid magnetic molecularly imprinted nano-microspheres of the present invention

[0032] 1.1 Preparation of carboxyl-modified magnetic nanospheres: Add ferric chloride hexahydrate with a mass concentration of 1.5-5% and sodium citrate dihydrate with a mass concentration of 0.5-2% to 100mL of ethylene glycol in turn, and react at 200°C for 12 After ~24 hours, the product was washed with deionized water and separated by magnetic field to obtain carboxyl-modified magnetic nanospheres. The particle size of the magnetic molecular imprinted nano microspheres prepared by the present invention is about 200nm.

[0033]1.2 Preparation of ursolic acid magnetic molecularly imprinted nanospheres: using ursolic acid as a template molecule, adding functional monomers, magnetic nanospheres, cross-linking agents and initiators to the solution in sequence, stirring mechanically at room temperature, and polymerizing After 12 hours, the reacti...

Embodiment 2

[0034] Example 2 Preparation of ursolic acid magnetic molecularly imprinted nanospheres

[0035] 2.1 (1) Add 3.25% ferric chloride hexahydrate and 1.0% sodium citrate dihydrate to 100 mL of ethylene glycol in turn, react at 200°C for 12 hours, wash the product with deionized water, and separate it with a magnetic field to obtain Carboxyl-modified magnetic nanospheres.

[0036] (2) Using ursolic acid as a template molecule, functional monomers, magnetic nanospheres, cross-linking agents and initiators were sequentially added to the solution, mechanically stirred at room temperature, and the reaction product was magnetically separated after 12 hours of polymerization, and deionized After washing with water and ethanol as reactants, ursolic acid magnetic molecularly imprinted nanospheres were prepared, and non-imprinted magnetic microspheres were prepared as a control;

[0037] Wherein, the consumption of ursolic acid is 10mg; Functional monomer is triaminopropyltriethoxysilane ...

Embodiment 3

[0045] The adsorption kinetic curve of magnetic molecularly imprinted nanospheres (MNP@MIP) prepared by the present invention for separating ursolic acid from Cornus officinalis to ursolic acid is determined as follows:

[0046] The ursolic acid magnetic molecularly imprinted nanosphere (0.2g) that 2.1 in embodiment 2 prepares is placed in 200mL concentration and is 100mg L -1 In the ursolic acid standard solution, the microspheres were collected by magnetic separation at 0, 5min, 10min, 15min, 20min, 25min, 30min, 35min, 40min, and 45min respectively, and the ultraviolet absorption value was measured at 210nm, and determined according to the ursolic acid standard curve The concentration of remaining ursolic acid in the solution was calculated, and the adsorption amount of molecularly imprinted microspheres to ursolic acid was calculated at different times. The specific test results are as follows: figure 2 shown.

[0047] Depend on figure 2 It can be seen that the adsorpt...

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Abstract

The invention belongs to the field of biological separation and natural product extraction, and particularly relates to ursolic acid magnetic molecularly imprinted nano-microspheres and a method for extracting ursolic acid from dogwood. The preparation method of the ursolic acid magnetic molecularly imprinted nano-microspheres comprises the following steps: (1) preparing carboxyl modified magneticnano-microspheres by taking 1.5-5 wt% of ferric chloride hexahydrate and 0.5-2 wt% of sodium citrate dihydrate as raw materials and ethylene glycol as a solvent and adopting a hydrothermal synthesismethod; and (2) preparing the ursolic acid magnetic molecularly imprinted nano-microspheres from raw materials including the magnetic nano-microspheres. The ursolic acid magnetic molecularly imprintednano-microspheres provided by the invention have large ursolic acid adsorption capacity, can realize rapid separation of a target object in complex components by controlling a magnetic field, can effectively avoid repeated centrifugation, filtration and other procedures, and can effectively solve the problem of high extraction difficulty of ursolic acid in dogwood.

Description

technical field [0001] The invention belongs to the field of biological separation and natural product extraction, and in particular relates to an ursolic acid magnetic molecular imprinted nano-microsphere and a method for extracting ursolic acid from dogwood. Background technique [0002] Cornus Officinalis Sieb.et Zucc., as a kind of Chinese herbal medicine, has the effects of invigorating the stomach, nourishing the liver and kidney, curing anemia, lumbago, and weakness of the nerves and heart. The main production areas in my country are western Henan, Zhejiang and other places. Ursolic acid (UA) is a pentacyclic triterpenoid compound in the main active ingredient of Cornus officinalis, which has physiological functions such as anti-inflammatory, anti-tumor, immune regulation, and antitussive. At present, the methods used to extract ursolic acid from plants mainly include leaching method, Soxhlet extraction method, ultrasonic method, resin adsorption method, enzymatic hy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/26C08G77/26C08K9/04C08K7/18B01J20/26B01J20/28B01J20/30B01D15/02C07J63/00C08L83/08
CPCC08J9/26C08G77/26C08K9/04C08K7/18B01J20/268B01J20/28009B01J20/28016B01D15/02C07J63/008C08K2201/01C08K2201/003C08J2383/08C08J2201/0422
Inventor 张鑫李彦松汤波
Owner NANYANG NORMAL UNIV
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