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Liquid-based fungus detection method based on flaking method

A detection method and fungal technology, applied in the field of medical detection, can solve the problems of complicated operation, long process time, and many reagents, etc., and achieve the effects of good fluorescence effect, prevention of loss of components, and improvement of visibility.

Pending Publication Date: 2020-06-05
江西业力医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a liquid-based fungus detection method based on a film-making method, the purpose of which is to improve the visibility of fungal cells, improve the observation efficiency of fungal cells, and can easily and quickly realize the filtering of impurities in samples to be tested, enrichment The fungus to be detected is made into slices for easy observation and detection, which effectively solves the problems of cumbersome operation, long process and many reagents required in the prior art

Method used

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  • Liquid-based fungus detection method based on flaking method
  • Liquid-based fungus detection method based on flaking method
  • Liquid-based fungus detection method based on flaking method

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A liquid-based fungal detection method based on tablet method, comprising the following steps:

[0031] S1. Sampling: The gynecologist collects the specimen with a disposable specimen collection brush, pinches the handle of the brush with the thumb and forefinger, inserts the tip of the collection brush into the cervix and rotates it clockwise for 3-5 weeks, pulls out the collection brush, and removes it. Immediately put the brush head into the specimen bottle containing the preservation solution, shake gently and rinse, so that all the cells on the brush head are mixed into the preservation solution;

[0032] The preservation solution is prepared from the following raw materials: 2 parts of traditional Chinese medicine antibacterial water extract, 2 parts of vitamin C, 1 part of stabilizer, 1 part of fixative, 10 parts of glycerol, an appropriate amount of acetic acid and 100 parts of deionized water. The acetic acid is used to adjust the pH value of the preservation s...

Embodiment 2

[0046] A liquid-based fungal detection method based on tablet method, comprising the following steps:

[0047] S1. Sampling: The gynecologist collects the specimen with a disposable specimen collection brush: pinch the handle of the brush with the thumb and forefinger, insert the tip of the collection brush into the cervix and rotate clockwise for 3-5 weeks, pull out the collection brush, and remove it. Immediately put the brush head into the specimen bottle containing the preservation solution, shake gently and rinse, so that all the cells on the brush head are mixed into the preservation solution;

[0048] The preservation solution is prepared from the following raw materials: 5 parts of antibacterial water extract of traditional Chinese medicine, 7 parts of vitamin C, 3 parts of stabilizer, 2 parts of fixative, 20 parts of glycerin, appropriate amount of acetic acid and 150 parts of deionized water. The acetic acid is used to adjust the pH value of the preservation solution...

Embodiment 3

[0062] A liquid-based fungal detection method based on tablet method, comprising the following steps:

[0063] S1. Sampling: The gynecologist collects the specimen with a disposable specimen collection brush: pinch the handle of the brush with the thumb and forefinger, insert the tip of the collection brush into the cervix and rotate clockwise for 3-5 weeks, pull out the collection brush, and remove it. Immediately put the brush head into the specimen bottle containing the preservation solution, shake gently and rinse, so that all the cells on the brush head are mixed into the preservation solution;

[0064] The preservation solution is prepared from the following raw materials: 3 parts of antibacterial water extract of traditional Chinese medicine, 5 parts of vitamin C, 2 parts of stabilizer, 1.5 parts of fixative, 15 parts of glycerol, appropriate amount of acetic acid and 125 parts of deionized water. The acetic acid is used to adjust the pH value of the preservation soluti...

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Abstract

The invention provides a liquid-based fungus detection method based on a flaking method, and belongs to the technical field of medical detection. The liquid-based fungus detection method specificallycomprises the following steps: S1, sampling; S2, sample treatment; S3, sample flaking; and S4, sample detection. A liquid-based fungus enrichment flaking method is adopted, the appearance degree of the fungal cells is improved, the observation efficiency of the fungal cells is improved, the impurity filtration in the sample to be detected and the enrichment of the fungus to be detected can be simply and rapidly achieved so as to produce the sheet so that the observation and the detection are easily achieved, and the problems of complex operation, long process time consumption, more required reagents and the like in the prior art are effectively solved.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a liquid-based fungal detection method based on a sheet-making method. Background technique [0002] Fungi belong to eukaryotes, without plastids, and their nutritional mode is absorption without phagocytosis. The cell wall contains chitin and β-glucan. It can cause human and animal diseases, called fungal diseases, and can also cause plant diseases, human allergic diseases and mycotoxin poisoning. Fungal invasion of the human body is often secondary to other diseases, such as chronic wasting diseases such as malignant tumors and diabetes; large doses of X-ray irradiation; immunosuppressants, etc. lead to low immune function and resistance; The reaction is mostly suppressed, and it is also easy to be infected with fungi; in addition, organ transplantation, catheter intubation, radiotherapy and other applications cause local tissue damage, which provides conditions for...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N21/64
CPCG01N1/2813G01N21/6486G01N2001/2846
Inventor 熊文芳吕正和陈永洪杜厚明杨平平侯莉赖文成
Owner 江西业力医疗器械有限公司
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