Fully enclosed cell culture method

Abstract

The present invention provides a fully enclosed cell culture method using a fully enclosed cell culture system, which adopts the following steps: 1) liquid feeding process; 2) building a culture environment: preset the concentration values ​​of oxygen and carbon dioxide, and inject oxygen into the incubator respectively and carbon dioxide, measure the gas concentration values ​​of oxygen and carbon dioxide in the incubator in real time, and compare them with the set values ​​respectively. When the oxygen and carbon dioxide values ​​in the incubator reach the standard, inject the gas in the incubator into the culture tank; 3) Continuous cultivation; 4) Replacement and concentration; 5) Recovery of finished products. At present, there is no automatic equipment specially used for CAR‑T cell culture in China. Compared with manual laboratory culture, this equipment can greatly save labor costs and significantly improve the culture efficiency.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a fully enclosed cell culture method. Background technique [0002] In recent years, CAR-T cell immunotherapy is considered to be one of the most promising treatments for cancer. It has many advantages that other therapies cannot match. For example, CAR-T cells can have multiple targeting sites, which can improve the accuracy of tumor treatment, and the action process is not limited by MHC (major histocompatibility complex); CAR-T cells kill The tumor range is wider, the effect is more durable; the technical attributes are strong, and the reproducibility is strong. In 2018, the FDA approved two CD19CAR-T cell drugs (Kymriah and Yescarta, respectively), and these two CAR-T cell drugs have achieved good results in the treatment of malignant hematological tumors. However, CAR-T cell immunotherapy still has many limitations, such as the preparation of CAR-T cells. In the proc...

AI Technical Summary

Problems solved by technology

[0004] Most of the current cell cultures are manually operated. When a large number of cells need to be cultured for industrialization, a lot of labor and time costs are required. At the same time, as the burden on the operator increases, the risk of error is also greatly increased; in addition, Artificial culture cannot accurately control the environment of cell growth, which is not conducive to cell growth

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