Rapid detection method, liquid chip and kit for four human infectious diseases

A liquid phase chip and infectious disease technology, which is applied in the fields of life science and biology, can solve the problems of decreased activity of cells and viruses, low degree of detection reaction, and inconspicuous color results, etc., to achieve high activity and increase the effect of detection

Pending Publication Date: 2020-05-29
武汉尚码生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a rapid detection method for four human infectious diseases, a liquid phase chip and a kit, which solves the problem of external environment and other factors after the detection sample is extracted due to in vitro operation. It is easy to reduce the activity of cells and viruses, resulting in a low degree of detection reaction, making the color development results not obvious, and the detection results cannot be obtained accurately.

Method used

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  • Rapid detection method, liquid chip and kit for four human infectious diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: 100 parts of sample diluent, 20 parts of oligonucleotide for HIV detection, 10 parts of HIV positive control, 0.1 part of insulin, 50 parts of amino acid, 0.5 part of DNA polymerase, 0.5 part of reverse transcriptase, RNA polymerase Mix 0.5 parts of enzyme, 10 parts of nucleic acid release agent, 5 parts of targeted nucleotide detection primers, 5 parts of TP positive control, 10 parts of anti-hepatitis B pre-S1 antigen monoclonal antibody and 5 parts of PCR amplification reaction reagent to prepare the solution Phase chip, sealed by the kit, then extract 100ml serum sample and add Trizol, let stand at room temperature for 5min, centrifuge at 1000g centrifuge for 10min at 10°C, extract the supernatant to another reagent tube, and centrifuge again at 10°C Centrifuge at 1000g for 10min, remove the supernatant, take out the centrifuged serum for later use, drop 25ml of centrifuged serum into the kit at room temperature, shake well and let it stand for 5min at 25°...

Embodiment 2

[0024] Example 2: 100 parts of sample diluent, 10 parts of oligonucleotides for HIV detection, 5 parts of HIV positive controls, 0.2 parts of insulin, 50 parts of amino acids, 0.5 parts of DNA polymerase, 0.5 parts of reverse transcriptase, RNA polymerase Mix 0.5 parts of enzyme, 20 parts of nucleic acid release agent, 10 parts of targeted nucleotide detection primers, 5 parts of TP positive control, 10 parts of anti-hepatitis B pre-S1 antigen monoclonal antibody and 5 parts of PCR amplification reaction reagent to prepare the solution Phase chip, sealed by the kit, then extract 100ml serum sample and add Trizol, let stand at room temperature for 5min, centrifuge at 1000g centrifuge for 10min at 10°C, extract the supernatant to another reagent tube, and centrifuge again at 10°C Centrifuge at 1000g for 10min, remove the supernatant, take out the centrifuged serum for later use, drop 25ml of centrifuged serum into the kit at room temperature, shake well and let it stand for 5min ...

Embodiment 3

[0025]Example 3: 100 parts of sample diluent, 10 parts of oligonucleotides for HIV detection, 5 parts of HIV positive controls, 0.1 parts of insulin, 20 parts of amino acids, 0.2 parts of DNA polymerase, 0.2 parts of reverse transcriptase, RNA polymerase Mix 0.2 parts of enzyme, 10 parts of nucleic acid release agent, 5 parts of targeted nucleotide detection primers, 5 parts of TP positive control, 20 parts of anti-hepatitis B pre-S1 antigen monoclonal antibody and 5 parts of PCR amplification reaction reagent to prepare the solution Phase chip, sealed by the kit, then extract 100ml serum sample and add Trizol, let stand at room temperature for 5min, centrifuge at 1000g centrifuge for 10min at 10°C, extract the supernatant to another reagent tube, and centrifuge again at 10°C Centrifuge at 1000g for 10min, remove the supernatant, take out the centrifuged serum for later use, drop 25ml of centrifuged serum into the kit at room temperature, shake well and let it stand for 5min at...

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Abstract

The invention discloses a rapid detection method, liquid chip and kit for four human infectious diseases, and relates to the technical field of life sciences and biology. The liquid chip mainly includes 60-100 parts of a sample diluent, 10-20 parts of oligonucleotides for HIV detection, 5-10 parts of HIV positive control, 0.1-0.2 part of insulin, 20-50 parts of amino acids, 0.2-0.5 part of DNA polymerase, 0.2-0.5 part of reverse transcriptase, 0.2-0.5 part of RNA polymerase, 10-20 parts of a nucleic acid releasing agent and 5-10 parts of nucleotide-targeted detection primers. According to therapid detection method, liquid chip and kit for the four human infectious diseases, high activity of cells and viruses can be maintained through coordination of the insulin and amino acids when AIDS,syphilis, hepatitis C and hepatitis B are detected, and the process of reactions of AIDS, syphilis, hepatitis C and hepatitis B viruses can be catalyzed and accelerated through combination of the DNApolymerase, reverse transcriptase and RNA polymerase, so that the detection effect of the detection kit is increased greatly, and the detection effect is more accurate.

Description

technical field [0001] The invention relates to the fields of life science and biotechnology, in particular to a rapid detection method for four human infectious diseases, a liquid phase chip and a kit. Background technique [0002] Infectious diseases are a class of diseases caused by various pathogens that can be transmitted between humans, animals and animals, or humans and animals. Most of the pathogens are microorganisms, and a small part of them are parasites. Parasites are also known as Parasitic diseases, some infectious diseases, the epidemic prevention department must grasp its incidence in time and take countermeasures in a timely manner. Therefore, after discovery, it should be reported to the local epidemic prevention department in time according to the specified time. There are 3 categories A, B, and C, with a total of 39 types. In addition, it also includes other infectious diseases that the National Health and Family Planning Commission has decided to include...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12Q1/44
CPCC12Q1/703C12Q1/707C12Q1/706C12Q1/701C12Q1/686C12Q1/44C12Q2600/166C12Q2545/101C12Q2521/101C12Q2521/107C12Q2521/119
Inventor 王华全
Owner 武汉尚码生物科技有限公司
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