Cosmetic composition for improving skin condition comprising plant cell complex cultures
A technology for cosmetic composition and cell culture, which can be applied to medical preparations containing active ingredients, cosmetics, pharmaceutical combinations, etc., and can solve the problems of increased activity and decreased synthesis of matrix metalloproteinase 1
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Embodiment 1
[0091] Embodiment 1: Preparation according to the plant cell complex composition of the present invention
Embodiment 1-1
[0092] Embodiment 1-1: Surface disinfection of 11 kinds of plant samples
[0093] For the production of the cells according to the invention, alpine edelweiss leaves, sea holly leaves, sea fennel leaves, apple leaves, grape leaves, desert rose leaves, lavender leaves, vitex leaves, lotus leaves, myrrh leaves, jasmine leaves, In order to sterilize the surface, it was immersed in 70% ethanol for 30 seconds, sterilized by shaking in 2% sodium hypochlorite (Sodium hypochlorite) for 5 minutes, and washed with sterilized water.
Embodiment 1-2
[0094] Example 1-2: Induction of callus from 11 plant samples
[0095] Calli from 11 plant samples were cultured as follows.
[0096] 1) Utilize a sharp knife to cut the sterilized edelweiss leaves at one time, and add 6-benzylaminopurine (6-Benzylaminopurine) as Plant Growth Regulators (Plant Growth Regulators) containing 1mg / L, 0.3mg / L In the basic MS medium (Murashige and Skoog 1962, Duchefa Company, Cat No.M0221) of 2, 4-D, 3% sucrose and 8g / L agar (agar), at 25 ℃, humidity is 70% growth Conditioned dark culture, and induction into primary plant cells. Each medium was adjusted to pH 5.8 with 1N sodium hydroxide (NaOH). Subculture was performed at 2-week intervals.
[0097] 2) Utilize a sharp knife to cut sterilized sea holly leaves at one time, and indole-3-acetic acid (indole-3-acetic acid, IAA), 5mg / L corn containing 50mg / L as a plant growth regulator The basic MS medium (Murashige and Skoog 1962, Duchefa company, Cat No.M0221) of the agar (Murashige and Skoog 1962) ...
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