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Application of Yanhuanglian and its preparations in the preparation of medicines for treating non-alcoholic fatty liver disease

A non-alcoholic, steatohepatitis technology, applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problem of no effective drug treatment, complex pathogenesis, etc., to expand clinical indications, prevent liver damage, improve drugs effect of liver injury

Active Publication Date: 2021-09-28
JIANGSU HONGDIAN RES INST OF TRADITIONAL CHINESE MEDICINE IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pathogenesis of non-alcoholic fatty liver disease is very complicated, and there is no recognized effective drug treatment at present, so the development of effective drugs based on the research on the pathogenesis still needs to continue to work

Method used

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  • Application of Yanhuanglian and its preparations in the preparation of medicines for treating non-alcoholic fatty liver disease
  • Application of Yanhuanglian and its preparations in the preparation of medicines for treating non-alcoholic fatty liver disease
  • Application of Yanhuanglian and its preparations in the preparation of medicines for treating non-alcoholic fatty liver disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Preparation of total alkaloids of Litectin

[0035] (1) Take 30kg of Yanhuanglian decoction pieces, add 10 times the volume concentration of 75% ethanol and soak for 1 hour;

[0036] (2) Then heat and reflux for extraction three times, each time for 2 hours, and combine the extracts;

[0037] (3) Recover as much ethanol as possible and concentrate to 1:1.3 (Rectinus rhizome W: Concentrate V) to obtain a concentrate;

[0038] (4) Take the above concentrated solution and add 1% hydrochloric acid which is 4 times the amount of the raw material (Rectinus root decoction pieces), put it in an acid-resistant multifunctional extraction tank and heat and reflux for extraction for 1 hour;

[0039] (5) Adjust the extract with 40% sodium hydroxide and keep the pH value to 8, then concentrate to a relative density of 1.06-1.08 (60°C), refrigerate the concentrate, let it stand for 48 hours, and filter; filter the precipitate for use Heat 1% hydrochloric acid 16 times the ...

Embodiment 2

[0043] Example 2 Observation of curative effect of Yanhuanglian total alkaloid capsules on cell experiments.

[0044] 1. Test drug: the total alkaloid extract (CSBTA) of Litectinus chinensis prepared in Example 1.

[0045] 2. Cytotoxicity experiment

[0046] 1.1 Experimental instrument: MULTSKAN Sky full-wavelength microplate reader (Thermo Scientific, USA)

[0047] 1.2 Reagents: MTT cell proliferation and cytotoxicity detection kit (article number: KGA321, Jiangsu Kaiji Biotechnology Co., Ltd.); total alkaloids of Litectinum prepared in Example 1;

[0048] 1.3 Experimental method: Add 100 µL / well of cells in a 96-well plate (about 1×10 4 ), set at 37℃, 5%CO 2 Cell culture incubator for 24 hours. Aspirate the medium of all wells, use the blank medium without adding serum to configure CSTBA (100ug / mL) solution, dilute it to seven concentrations of 50, 25, 12.5 and so on, and mix the blank medium with Each concentration of medium was added to a 96-well plate and placed at 3...

Embodiment 3

[0050] Example 3 Observation of the curative effect of total alkaloids of Litectinum chinensis on hypolipidemic effect of hyperlipidemia model rats

[0051]1. Experimental modeling

[0052] 1.1 Experimental animals: male C57BL / 6 black mice, 4 weeks old, weighing about 19g, purchased from the Comparative Medicine Center of Yangzhou University.

[0053] 1.2 Feed: 60% high-fat feed, fructose, and normal feed were purchased from Nantong Trophy Feed Technology Co., Ltd.

[0054] 1.3 Reagents: the total alkaloids extract of Litectinus rhizome prepared in Example 1.

[0055] 1.4 Experimental method: Take 35 male C57BL / 6 small black mice, 4 weeks old, after one week of adaptive feeding, divide the mice into two initial groups randomly, and feed them with normal diet or high-fat (60% fat) high-sugar Diet (20% fructose). After feeding for 10 weeks, the mice were randomly divided into model groups, total alkaloids of Litectin (25mg / kg), total alkaloids of Litectin (100mg / kg), metformi...

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Abstract

The invention discloses the application of rock rhizome and its preparation in treating non-alcoholic fatty liver disease and / or non-alcoholic steatohepatitis. Through a large number of experimental screenings, the present invention finds that total alkaloids of Litectin can reduce the synthesis of fatty acids in cells, and can reduce the levels of TG, TC, LDL cholesterol, HDL cholesterol and free fatty acids induced by HFD, and animal anatomy experiments show that total alkaloids of Litectin It can effectively reduce the weight of the liver, and the pathological section shows that the liver tissue is well protected. And through clinical experiments, it was found that Yanhuanglian total alkaloid capsules can significantly improve the biochemical indicators and imaging evaluation results of patients with fatty liver. In addition, the results of the drug-induced liver injury test in the present invention show that the total alkaloid capsule of Lithocarpus can significantly improve the drug-induced liver injury and has the effect of preventing liver injury.

Description

technical field [0001] The present invention relates to a new application of Yanhuanglian and Yanhuanglian total alkaloid capsules, in particular to the application of Yanhuanglian and its Yanhuanglian total alkaloid capsules in the treatment of nonalcoholic fatty liver disease and nonalcoholic steatohepatitis. field of medical technology. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is defined as hepatic steatosis confirmed by imaging and liver histology, and other causes of hepatic steatosis have been excluded, such as heavy drinking, long-term use of pro-adipogenic drugs or Single-gene genetic disorders, etc., according to their clinical manifestations, mainly include hepatic steatosis, non-alcoholic steato-hepatitis (non-alcoholic steato-hepatitis, NASH) and liver cirrhosis. The pathogenesis of non-alcoholic fatty liver disease is very complicated, and there is no recognized effective drug treatment at present, so the development of effective ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/66A61K9/48A61P1/16
CPCA61K36/66A61K9/48A61P1/16A61K2236/333A61K2236/39A61K2236/53A61K2236/51A61K36/505
Inventor 成俊刁和芳黄芳郑艳萍赵开军
Owner JIANGSU HONGDIAN RES INST OF TRADITIONAL CHINESE MEDICINE IND CO LTD
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