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Multispectral laminated imaging method based on aperture scanning

A stacked imaging, multi-spectral technology, applied in the field of optical computing, can solve problems such as errors and affect the quality of high-resolution images, and achieve the effect of reducing requirements, improving imaging quality, and reducing aberrations

Active Publication Date: 2020-05-08
HANGZHOU DIANZI UNIV
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AI Technical Summary

Problems solved by technology

However, if the spatial pupil function of this method has not been updated, it will cause errors and affect the quality of the final synthesized high-resolution image

Method used

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  • Multispectral laminated imaging method based on aperture scanning
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  • Multispectral laminated imaging method based on aperture scanning

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Embodiment Construction

[0023] A multi-spectral stack imaging method based on aperture scanning, specifically comprising the following steps:

[0024] Step 1. Build the equipment. Use an objective, a circular aperture, a diffraction grating, a lens, an encoding mask and a monochrome camera with an LED light source. The lens has a focal length of 50 mm; the circular aperture has a radius of 2.5 mm; and a monochrome camera with a pixel size of 3.45 µm is used to capture the sample spectrum. Place the objective lens, circular aperture, diffraction grating, lens, encoding mask and monochrome camera in sequence. The LED light is placed in front of the objective lens, and the sample is placed between the objective lens and the LED light, and the circular hole can be scanned and moved. Specific visible figure 2 .

[0025] Step 2. Initially assume a high-resolution image I(u).

[0026] Step 3. It is mentioned in the previous construction of the equipment that the aperture can move and scan circularly. ...

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Abstract

The invention relates to a multispectral laminated imaging method based on aperture scanning. An LED matrix is replaced by aperture scanning, single-beam light irradiation is adopted, light waves emitted from a sample pass through an aperture, low-resolution images from different positions of the sample are obtained through aperture cyclic scanning and hyperspectral processing, processing is carried out in a Fourier domain, and finally a high-resolution image of the sample is obtained. On the premise of realizing laminated imaging, the defects of an LED matrix are overcome. And the pupil function and the sample spectrum are iteratively updated, and the pupil function is converged, so that the resolution of the obtained imaging picture is higher. Hyperspectral imaging is introduced, and thenature of the sample is further understood through hyperspectral imaging, so that a clearer image is constructed, the sample can be analyzed more thoroughly, and conditions are provided for analyzingthe sample.

Description

technical field [0001] The invention relates to the field of optical computing, in particular to a multi-spectral stacked imaging method based on aperture scanning. Background technique [0002] Fourier stack microscopic imaging technology is a new computational imaging technology in the past ten years. It overcomes the problem that previous microscopes cannot take into account both large field of view and high resolution at the same time, and has many advantages such as large field of view, high resolution, and quantitative phase imaging. Since the lamination imaging technology was proposed in 2013, it has attracted the attention of various fields such as optical microscopy, biomedicine, and life sciences, and has been widely used. In this method, the phase recovery iterative algorithm is used to find the unique complex solution that satisfies the constraints of multiple far-field diffraction intensity images in the overlapping scanning mode. The resolution of this imagin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01J3/28G01N21/25
CPCG01J3/2823G01N21/25
Inventor 颜成钢姚婷婷方运志孙垚棋张继勇张勇东韩卫东沈韬
Owner HANGZHOU DIANZI UNIV
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