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A Strain of Bacillus and Its Application in Industry

A bacillus and strain bud technology, applied to bacillus and its application in industry, can solve the problems of low product content, low production efficiency, inconsistent optimal conditions, etc., and achieve high yield, sufficient nutrition, and shortened fermentation cycle. Effect

Active Publication Date: 2021-05-11
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

In this method, on the one hand, the optimal conditions for enzyme production and enzymolysis are inconsistent, resulting in low production efficiency; on the other hand, part of the fucoidan oligosaccharides produced during the fermentation process will be utilized by the bacteria, resulting in low product content

Method used

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  • A Strain of Bacillus and Its Application in Industry
  • A Strain of Bacillus and Its Application in Industry
  • A Strain of Bacillus and Its Application in Industry

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Embodiment Construction

[0030] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0031] 1. Strain screening, identification and construction of phylogenetic tree

[0032] 1. Strain screening

[0033] Prepare MAlg medium (selective medium): glucose 3g / L, sodium alginate 3g / L, peptone 3g / L, yeast extract powder 6g / L, agar 20g / L, and tap water.

[0034] Prepare the seed medium: glucose 5g / L, peptone 5g / L, yeast extract powder 10g / L, seawater preparation.

[0035] On March 20, 2018, we collected 4 rotten kelp trees from the Yellow Sea, put the 4 rotten kelp trees together for homogenization, and took the supernatant and diluted it in gradients (10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 ) were spread on the MAlg plate medium respectively, and the corresponding plates were respectively recorded as 1P, 2P, 3P, 4P, 5P, 6P, 7P, and 8P. These 8 plates were cultured upside down at a constant temperature of ...

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Abstract

The invention discloses a strain of Bacillus and its application in industry. The strain screened rotten kelp picked up from the Yellow Sea and was preserved in the China Center for Type Culture Collection on April 28, 2019. The preservation number is: CCTCC No. .M 2019315, which can produce alginate lyase, and the enzymatic activity of the produced alginate lyase can adapt to a wide temperature range, between 30-45°C. The enzyme activity of the alginate lyase produced by the strain screened by the present invention is suitable for a wide temperature range and has certain advantages in the industry. The fermentation medium specially developed for the strain with the preservation number of CCTCC No.M 2019315 has sufficient nutrition. The strain can grow at a high density, so it produces more alginate lyase, has a stronger ability to degrade alginate, and has a higher yield of the product fucoidan oligosaccharide.

Description

technical field [0001] The present invention relates to a strain of bacillus and its application in industry, in particular to a strain of bacillus capable of producing alginate lyase and whose enzymatic activity is suitable for a wide temperature range, and the application of the strain in industry——using the strain to prepare brown algae The oligosaccharide belongs to the field of marine biotechnology. Background technique [0002] Alginate is a linear acidic polysaccharide composed of α-L guluronic acid and β-D mannuronic acid, which is widely present in the cell walls of large brown algae such as kelp and wakame. The degradation product of alginate, alginate oligosaccharides, has various biological activities, such as lowering blood fat, anti-virus, anti-tumor and anti-oxidation. At present, there are two methods for producing fucoidan oligosaccharides: one-step method and two-step method. The one-step method refers to the direct completion of the fermentation producti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/88C12P19/12C12P19/04C12P19/00C12R1/07
CPCC12N1/20C12N9/88C12P19/00C12P19/04C12P19/12C12Y402/02C12N1/205C12R2001/07
Inventor 刘正一秦松宋宛霖李莉莉俞燕洁
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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