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Application of lactobacillus acidipiscis for preparing medicine capable of preventing or treating autoimmune diseases

An autoimmune and lactobacillus technology, applied in the direction of allergic diseases, metabolic diseases, bone diseases, etc., can solve the problems of toxic and side effects of patients, and achieve the effect of solving recurrence, mature amplification technology and easy to accept

Inactive Publication Date: 2020-05-01
STOMATOLOGY AFFILIATED STOMATOLOGY HOSPITAL OF GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the causes and molecular mechanisms of the abnormalities of Treg cells and Th17 cells in MS patients are still poorly understood, and most of the existing drugs for the treatment of multiple sclerosis are hormones, which will cause great toxic side effects to patients

Method used

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  • Application of lactobacillus acidipiscis for preparing medicine capable of preventing or treating autoimmune diseases
  • Application of lactobacillus acidipiscis for preparing medicine capable of preventing or treating autoimmune diseases
  • Application of lactobacillus acidipiscis for preparing medicine capable of preventing or treating autoimmune diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Changes in the composition of intestinal flora in EAE mice

[0035] 1. Experimental method

[0036] use MOG 35-55 Peptide immunization of mice in CD44KO (CD44KO mice) group and WT (C57BL / 6 wild-type mice) group to establish EAE model: subcutaneously inject 200uL of CFA containing 150μg MOG into the mice 35-55 and inactivated Mycobacterium tuberculosis (6 mg / mL); on day 0 (the same day) and day 2 after immunization, 200 ng and 400 ng of pertussis toxin were intraperitoneally injected into mice, respectively.

[0037]Fecal samples from mice in the WT group and CD44KO group were collected, and the improved CTAB method was used to extract the total DNA in the fecal samples. The concentration and purity of the extracted DNA were determined using Nanodrop 2000 (Thermo Scientific). 35-55 On the 15th day after peptide immunization, the 16srRNAV4 of the intestinal flora of the mice was sequenced and analyzed to determine the changes in the composition of the intestin...

Embodiment 2

[0040] Example 2 Effect of Lactobacillus acidfish 10851 on differentiation of Treg cells, TH1 cells and TH17 cells

[0041] CD4 helper T cells (T helper, Th) are considered to play the most important role in the pathogenesis of MS. Th cells have appeared in the early stage of lesion formation; Th cells themselves contain many subtypes, such as Th1, Th2, Th17, It is known that CD4+ T cell subsets TH1 and TH17 promote inflammation, while TH2 and Treg can inhibit the occurrence of inflammation. Cytokines are small molecular polypeptides or glycoproteins synthesized and secreted by various tissue cells (mainly immune cells), and have biological functions such as regulating cell growth, differentiation and maturation, function maintenance, immune response regulation, and participation in inflammatory reactions. , IFN-γ and IL-17 are common inflammatory cytokines, which have pro-inflammatory effects and promote the differentiation of CD4+ T cells into TH1 and TH17 inflammatory subpo...

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Abstract

The invention discloses application of lactobacillus acidipiscis for preparing a medicine capable of preventing and / or treating autoimmune diseases. The invention firstly provides the lactobacillus acidipiscis, Treg cell differentiation is obviously accelerated, TH1 cell or TH17 cell differentiation is inhibited, the generation of cell factors IFN (interferon)-[gamma] or IL-17 (interleukin 17) isinhibited, the generation of cell factors IL-10 or IL-13 is accelerated, and the inflammatory reaction of the autoimmune diseases is obviously reduced. The invention also provides a medicine for treating the autoimmune diseases. The medicine comprises an effective dosage of lactobacillus acidipiscis. After a patient takes the medicine, an obvious effect for treating the autoimmune diseases is achieved, no toxic and side effects are generated, and in addition, the lactobacillus acidipiscis can be conveniently obtained. Therefore, the lactobacillus acidipiscis has a wide application prospect inthe medicine capable of treating the autoimmune diseases.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the application of Lactobacillus acidicus in the preparation of medicines for preventing or treating autoimmune diseases. Background technique [0002] Autoimmune disease (autoimmune disease, AID) refers to a type of disease induced by the body's immune system's immune tolerance to its own components being broken, thereby attacking its own organs, tissues or cells, causing damage; for example, multiple sclerosis , Graves' disease, autoimmune liver disease, type I diabetes, systemic lupus erythematosus, rheumatoid arthritis, Sjogren's syndrome, etc. Abnormalities in the number and / or function of T cells play a crucial role in the pathogenesis of autoimmune diseases, among which regulatory T cells (Regulatory cells, Tregs) are a kind of T cell subsets that control autoimmune reactivity in the body. Treg group, which can inhibit their own inflammatory response,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P37/02A61P25/00A61P19/02A61P29/00A61P3/10A61P1/16A61P1/04A61P17/00
CPCA61K35/747A61P37/02A61P25/00A61P19/02A61P29/00A61P3/10A61P1/16A61P1/04A61P17/00
Inventor 管红兵
Owner STOMATOLOGY AFFILIATED STOMATOLOGY HOSPITAL OF GUANGZHOU MEDICAL UNIV
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