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Plasminogen activator detection kit and preparation method thereof

A detection kit and plasminogen technology, applied in the biological field, can solve the problems of poor sensitivity and repeatability, long detection time, poor thermal stability, etc., and achieve the effect of good stability, high sensitivity and guaranteed performance

Inactive Publication Date: 2020-04-24
苏州普瑞斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing reagents for detecting plasminogen activator have defects of poor sensitivity and repeatability, long detection time, low accuracy, and poor thermal stability, which limit its application

Method used

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  • Plasminogen activator detection kit and preparation method thereof
  • Plasminogen activator detection kit and preparation method thereof
  • Plasminogen activator detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A detection kit for plasminogen activator, including reagent R1 and reagent R2, the components and concentrations of the reagent R1 include: sodium dihydrogen phosphate dihydrate and disodium hydrogen phosphate dodecahydrate as buffer solutions , the concentration of sodium dihydrogen phosphate dihydrate is 1.6g / L, the concentration of disodium hydrogen phosphate dodecahydrate is 8g / L, also includes sodium chloride as an electrolyte, its concentration is 5.0g / L, as a stabilizer Bovine serum albumin at a concentration of 1 g / L, and cetyltrimethylammonium bromide at a concentration of 1 g / L, polyoxyethylene-20-oleyl ether at a concentration of 5 ml / L at a concentration of 1 g / L Sodium carboxymethyl cellulose, Proclin950 as preservative, its concentration is 1.5ml / L;

[0055]The components and concentrations of the reagent R2 include: latex microsphere antibody conjugate 1%, 2-(N-morpholine) ethanesulfonic acid monohydrate as coupling buffer, its concentration is 8g / L, Bo...

Embodiment 2

[0074] A detection kit for plasminogen activator, including reagent R1 and reagent R2, the components and concentrations of the reagent R1 include: sodium dihydrogen phosphate dihydrate and disodium hydrogen phosphate dodecahydrate as buffer solutions , the concentration of sodium dihydrogen phosphate dihydrate is 3g / L, the concentration of disodium hydrogen phosphate dodecahydrate is 16g / L, also includes potassium chloride as an electrolyte, its concentration is 10g / L, mannitol as a stabilizer , its concentration is 3g / L, and the concentration is 4g / L cetyltrimethylammonium bromide, the concentration is 10ml / L polyoxyethylene-20-oleyl ether, the concentration is 3g / L carboxymethyl Sodium cellulose, Proclin300 as preservative, its concentration is 0.8ml / L;

[0075] The components and concentrations of the reagent R2 include: latex microsphere antibody conjugate 0.05%, 2-(N-morpholine) ethanesulfonic acid monohydrate as a coupling buffer, the concentration of which is 9g / L, Bo...

Embodiment 3

[0094] A detection kit for plasminogen activator, including reagent R1 and reagent R2, the components and concentrations of the reagent R1 include: sodium dihydrogen phosphate dihydrate and disodium hydrogen phosphate dodecahydrate as buffer solutions , the concentration of sodium dihydrogen phosphate dihydrate is 4.8g / L, the concentration of disodium hydrogen phosphate dodecahydrate is 24g / L, also includes magnesium chloride as an electrolyte, its concentration is 15g / L, glucose as a stabilizer, its The concentration is 5g / L, and the concentration is 8g / L cetyltrimethylammonium bromide, the concentration is 15ml / L polyoxyethylene-20-oleyl ether, the concentration is 5g / L carboxymethylcellulose Sodium, as preservative sodium azide, its concentration is 2.0ml / L;

[0095]The components and concentrations of the reagent R2 include: latex microsphere antibody conjugate 0.5%, 2-(N-morpholine) ethanesulfonic acid monohydrate as a coupling buffer, the concentration of which is 11g / L,...

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Abstract

The invention relates to a plasminogen activator detection kit. The kit is characterized by comprising a reagent R1 and a reagent R2; the reagent R1 comprises a first buffer solution A, a first buffersolution B, an electrolyte, a stabilizer, hexadecyl trimethyl ammonium bromide, polyoxyethylene-20-oleyl ether, sodium carboxymethyl cellulose and a preservative; and the reagent R2 comprises a latexmicrosphere antibody conjugate, a coupling buffer solution, a confining liquid and a storage liquid. The detection kit has the advantages that hexadecyl trimethyl ammonium bromide, polyoxyethylene-20-oleyl ether and sodium carboxymethyl cellulose are added into the reagent R1 at the same time, the sensitivity of the reagent can be effectively improved, the preservation period of the reagent is prolonged, the linear range is widened, and the accuracy of a detection result is guaranteed.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection kit for plasminogen activator and a preparation method. Background technique [0002] Plasminogen activator is a single-chain glycoprotein, which is mainly synthesized, secreted, and continuously released into the blood by vascular endothelial cells, and widely exists in various tissues of the body. main venue. It has a high affinity to fibrin, and then converts tyrosine plasminogen into plasmin, degrades fibrin (original) and some coagulation factors, and is the key substance of the fibrinolytic system. A large number of studies in recent years have proved that plasminogen activator, as a new type of marker, is closely related to the pathological process and prognosis evaluation of many diseases in the content changes in body fluids. Prompt fibrinolytic activity decreased, seen in the prethrombotic state and thrombotic diseases, such as arterial thrombosis, deep vein t...

Claims

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Application Information

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IPC IPC(8): G01N33/573G01N33/543
CPCG01N33/573G01N33/54313G01N2333/972
Inventor 袁嘉扬张凤
Owner 苏州普瑞斯生物科技有限公司
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