Preparation method of paper-based ratio photoelectrochemical biosensor for detecting microRNA
A biosensor, photoelectrochemical technology, applied in the direction of biochemical equipment and methods, electrochemical variables of materials, measurement/inspection of microorganisms, etc., can solve the problem of low microRNA content, improve signal amplification ability, reduce internal and external interference, The effect of accelerating the separation and transfer of photogenerated charges
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[0020] Example 1: Application of paper-based ratio photoelectrochemical biosensor in detecting microRNA-141
[0021] (1) First, use Adobe illustrator CS4 software to design the batik patterns of the paper auxiliary board and the paper detection board, and then print the designed batik patterns on the chromatogram paper through the wax printer model Color Qube 8580, and finally print the wax The chromatographic paper is heated in an oven at 130 ℃ for 50 s to obtain the hydrophilic auxiliary area of the paper auxiliary board, the hydrophilic working electrode area and the internal reference electrode area of the paper detection board;
[0022] (2) The hydrophilic working electrode area and the internal reference electrode area of the paper test board obtained in step (1) are functionalized gold nanoparticle conductive layers. The specific steps are as follows: first prepare gold seeds, and add 100 μL of 0.24 M Mercaptoethylamine was added to 10 mL of 1.5 mM chloroauric acid sol...
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