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Preparation method of paper-based ratiometric photoelectrochemical biosensor for detecting microRNA

A biosensor, photoelectrochemical technology, applied in the direction of biochemical equipment and methods, material electrochemical variables, microbial measurement/inspection, etc., can solve the problem of low microRNA content, improve signal amplification ability, realize signal amplification, reduce Effect of Small False Positive/Negative Signals

Inactive Publication Date: 2021-10-26
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, the content of microRNA is low, in order to achieve quantitative detection of microRNA, it is necessary to improve the accuracy and sensitivity of the analysis method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Application of paper-based ratiometric photoelectrochemical biosensor in detecting microRNA-141

[0021] (1) First, use Adobe illustrator CS4 software on the computer to design the batik pattern of the paper auxiliary board and the paper test board, then print the designed batik pattern on the chromatographic paper through a wax printer model of Color Qube 8580, and finally print the wax The chromatographic paper was heated in an oven at 130 °C for 50 s to obtain the hydrophilic auxiliary area of ​​the paper auxiliary plate and the hydrophilic working electrode area and internal reference electrode area of ​​the paper detection plate;

[0022] (2) The hydrophilic working electrode area and the internal reference electrode area of ​​the paper detection plate obtained in step (1) are functionalized with gold nanoparticles conductive layer, the specific steps are as follows: first prepare gold seeds, 100 μL concentration of 0.24 M Mercaptoethylamine was added to...

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Abstract

The invention discloses a preparation method of a paper-based ratio photoelectrochemical biosensor for detecting microRNA. Gold nanoparticles were grown on the paper-based double-electrode surface consisting of a working electrode and an internal reference electrode, and then cuprous oxide was electrodeposited and graphene quantum dots and silver iodide nanoparticles were sensitized to enhance the photocurrent signal; in the presence of target microRNA, the The DNA probes output from the double-strand specific nuclease reaction induced by different concentrations and constant concentrations of microRNA were respectively incubated on the surface of the working electrode and the internal reference electrode, which combined the DNA bridging strands and the DNA hairpins H1 and H2 on the electrode surface to induce the formation of DNA bridges The nanostructure causes the silver iodide nanoparticles labeled at the ends of H1 and H2 to stay away from the electrode surface, reducing the photocurrent signal, and realizing the sensitive detection of microRNA based on the ratio of the working current signal and the internal reference current signal.

Description

technical field [0001] The invention relates to the field of photoelectrochemical signal detection technology, paper-based sensing device preparation technology, nanocomposite material synthesis technology and microRNA analysis and detection technology, and more specifically to the preparation of a paper-based ratiometric photoelectrochemical biosensor for detecting microRNA method. Background technique [0002] In recent years, based on the comprehensive advantages of microfluidic paper analysis devices and photoelectrochemical analysis methods, such as low cost, portability, simple operation, and high sensitivity, various paper-based photoelectrochemical analysis devices have been prepared. In the process of paper-based photoelectrochemical analysis, a single-signal readout mode is usually used. This single-signal analysis strategy is susceptible to interference from the external environment, analytical equipment, target analytes, etc., resulting in false positive / negative...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/38G01N27/327C12Q1/6825
CPCC12Q1/6825G01N27/301G01N27/305G01N27/308G01N27/3272G01N27/3273G01N27/3278G01N27/38
Inventor 于京华杨红梅李正林胡孟苏张彦张丽娜葛慎光
Owner UNIV OF JINAN
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