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Method for determining pesticide residues in edible plant enzymes

A technology for edible plants and pesticide residues, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of single type of testing items, complicated processing process, and large solvent consumption, and achieves solvent saving, strong selectivity, and elimination of The effect of matrix interference

Pending Publication Date: 2020-04-14
广电计量检测(湖南)有限公司 +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Most of the current existing detection standards for pesticide residues in plant foods use SPE solid-phase extraction, which consumes a lot of solvents during the detection process and the pretreatment process is relatively complicated.
The types of detection items are single, and there is no method in the existing data that uses liquid chromatography in series with triple quadrupole mass spectrometry to simultaneously determine multiple pesticide residues in edible plant enzymes

Method used

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  • Method for determining pesticide residues in edible plant enzymes
  • Method for determining pesticide residues in edible plant enzymes
  • Method for determining pesticide residues in edible plant enzymes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]1. Principle of detection method:

[0032] The edible plant enzyme sample was extracted by 0.1% formic acid-acetonitrile (volume ratio 1:1) acidification system, and was extracted by compound C 18 and PSA filler QuEChERS dispersive extraction and purification, and finally use liquid chromatography triple quadrupole mass spectrometry to simultaneously determine the residues of 65 pesticides, use blank sample matrix as standard, retention and ion fragment abundance ratio time qualitative, quantitative ion peak area Quantification by standard method.

[0033] 2. Detection method procedure:

[0034] 3.1. Instruments and reagents

[0035] 3.1.1 Liquid chromatography triple quadrupole tandem mass spectrometer (AB Triple Quad 4500)

[0036] 3.1.2 Standard substances: 65 pesticide standard substances were purchased from Ehrensyorfer, Germany

[0037] 3.1.3 Analytical reagent: formic acid (China Resources Chemical, 500mL)

[0038] 3.1.4 Chromatographically pure reagent: acet...

Embodiment 2

[0095] 1. In order to explore the influence of different extraction solvents on the extraction efficiency, find a better extraction solvent for the determination of 65 kinds of pesticides in edible plant enzymes, take 2g of edible plant enzyme powder samples, and compare 10mL acetonitrile, 0.1% formic acid water, The extraction effect of 0.1% formic acid water + acetonitrile (volume ratio 1:1) three kinds of extraction solvents on 65 kinds of pesticides, with 0.05mg / kg standard addition recovery experiment (extraction solution is not purified), compare the effect of different extraction solvents on pesticides See Table 10 for extraction efficiency and the recovery distribution of 65 kinds of pesticides in edible plant enzymes.

[0096] Table 10

[0097]

[0098] It can be seen from the above data that when 0.1% formic acid water + acetonitrile (volume ratio 1:1) is used as the extraction solvent of 65 kinds of pesticides in edible plant enzymes, the recoveries of 39 kinds o...

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Abstract

The invention discloses a method for determining pesticide residues in edible plant enzymes, which comprises the following steps: (1) preparing a pesticide standard stock solution from a pesticide standard substance, carrying out QuEChERS pretreatment on plant enzymes without pesticide residues, and preparing a standard working curve by using liquid chromatography triple quadrupole tandem mass spectrometry; (2) carrying out QuEChERS pretreatment on the edible plant enzyme to be analyzed to obtain a filtrate; and (3) carrying out liquid chromatography triple quadrupole tandem mass spectrometryanalysis and determination on the filtrate, and analyzing to obtain the content of pesticide residues in the edible plant enzyme. According to the method disclosed by the invention, the QuEChERS and the liquid chromatography-tandem triple quadrupole mass spectrometry are used for determining the pesticide residues of the edible plant enzyme for the first time; at least 65 kinds of pesticide residues can be detected at the same time, an important theoretical basis is provided for detection of pesticide residues in edible plant enzymes in the future, and reference value is achieved for formulation of related pesticide residue detection standards.

Description

technical field [0001] The invention relates to a method for detecting pesticide residues, in particular to a method for measuring pesticide residues in edible plant enzymes. Background technique [0002] Preventive health care has become the national pursuit of health needs. In the field of preventive health care, every new nutritional functional food will open a new era of consumption, and the same is true for edible plant enzymes. Edible plant enzymes are developed by natural fermentation of dozens of different fruits and vegetables, grains, seaweeds and mushrooms. They retain the nutritional essence of the plants and contain various enzymes, oligosaccharides and various enzymes needed by the human body. Vitamins and minerals can also produce rich SOD antioxidant enzyme components, improve the body's antioxidant capacity, and then enhance immunity. Edible plant enzymes have a wide range of uses: promote metabolism, decompose aging necrotic cells, create new cells, and m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/72
CPCG01N30/02G01N30/06G01N30/14G01N30/72
Inventor 李丽蓝梦哲林绪陈惠琴袁浪
Owner 广电计量检测(湖南)有限公司
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