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Bletilla striata oligosaccharide extracted from bletilla striata rhizomes and preparation method of bletilla striata oligosaccharide

A technology of oligosaccharides and stems of Bletilla striata, which is applied to the preparation of oligosaccharides and sugar derivatives, chemical instruments and methods, etc., and can solve problems that have not been reported

Inactive Publication Date: 2020-04-14
HUNAN NUSTREETCARAX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, there are many methods for extracting, purifying, and preparing polysaccharides in Bletilla striata, but the research on the extraction of oligosaccharides in Bletilla striata has not been reported, especially how to extract and prepare Bletilla striata oligosaccharides using production guidelines that meet health and environmental protection. And the method that makes the obtained Bletilla striata oligosaccharides dissolve without obvious peculiar smell has no public reports, therefore, it is of great significance to develop a method for extracting Bletilla striata oligosaccharides from the rhizome of Bletilla striata

Method used

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  • Bletilla striata oligosaccharide extracted from bletilla striata rhizomes and preparation method of bletilla striata oligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for extracting bletilla striata oligosaccharides from rhizomes of bletilla striata, comprising the following steps:

[0028] (1) Wash 500kg of bletilla striata stems and cut them into 1-2mm thin slices; perform 3 extractions, add 3000L deionized water each time according to the ratio of solid to liquid ratio 1g: 6ml, stir and heat at 40°C for 3h; combine the extracts , and centrifuged to obtain the stem block extract of Bletilla striata;

[0029] (2) Pass the bletilla striata stem block extract at a speed of 1.5Bv / h through a neutral alumina column with a column volume of 2m3 and a column height-to-inner diameter ratio of 10:1 to obtain effluent A, wherein the neutral alumina column uses Dry column packing, the mesh number of alumina is 60-80 mesh;

[0030] (3) Concentrate the effluent A to Brix 15° in vacuum, pass through the cross-linked sephadex column G-15 with a column volume of 2m3 and a column height-to-inner diameter ratio of 15:1 at a speed of 2Bv / h, ...

Embodiment 2

[0035] The method for extracting bletilla striata oligosaccharides from bletilla striata rhizome comprises the following steps:

[0036] (1) Wash 380kg of bletilla striata stems and cut them into 1-2mm thin slices; perform 3 extractions, add 1900L deionized water each time according to the ratio of solid-liquid ratio 1g: 5ml, stir and heat at 40°C for 3h; combine the extracts , and centrifuged to obtain the stem block extract of Bletilla striata;

[0037] (2) Pass the bletilla striata stem extract at a rate of 1Bv / h through a neutral alumina column with a column volume of 2m3 and a column height-to-inner diameter ratio of 10:1 to obtain effluent A, wherein the neutral alumina column is dry Column packing by method, the mesh number of alumina is 60-80 mesh;

[0038] (3) Concentrate the effluent A to Brix 17° in vacuo, and pass through the cross-linked sephadex column G-15 with a column volume of 1.52m and a column height to internal diameter ratio of 10:1 with a speed of 1.5Bv...

Embodiment 3

[0043] The method for extracting bletilla striata oligosaccharides from bletilla striata rhizome comprises the following steps:

[0044] (1) Wash 450kg of bletilla striata stems and cut them into 1-2mm thin slices; extract 3 times, add 2475L deionized water each time according to the ratio of solid-liquid ratio 1g: 5.5ml, stir and heat at 46°C for 3h; combine and extract solution, centrifuged and filtered to obtain the stem block extract of Bletilla striata;

[0045] (2) Pass the bletilla striata stem block extract at a speed of 1.2Bv / h through a neutral alumina column with a column volume of 2m3 and a column height-to-inner diameter ratio of 10:1 to obtain effluent A, wherein the neutral alumina column uses Dry column packing, the mesh number of alumina is 60-80 mesh;

[0046] (3) Concentrate the effluent A to Brix 16° in vacuum, pass through the cross-linked sephadex column G-15 with a column volume of 1.8m3 and a column height-to-inner diameter ratio of 12:1 at a speed of ...

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Abstract

The invention discloses bletilla striata oligosaccharide extracted from bletilla striata rhizomes and a preparation method of the bletilla striata oligosaccharide. The method comprises the following steps: 1) extraction: slicing bletilla striata tubers, adding deionized water for multiple times of extraction, combining extracting solutions, and performing centrifugal filtration to obtain a bletilla striata tuber extracting solution; 2) alumina column treatment: treating the bletilla striata tuber extracting solution with a neutral alumina column to obtain an effluent A; 3) cross-linked dextrangel column treatment: concentrating the effluent A, loading the concentrated effluent A to a treated cross-linked dextran gel column for adsorption separation, eluting with deionized water, and collecting eluent to obtain effluent B; and 4) drying: concentrating and freeze-drying the effluent B to obtain the bletilla striata oligosaccharide. The bletilla striata polysaccharide is decolorized anddeodorized through the neutral aluminum oxide and the cross-linked dextran gel column, polysaccharide and free proteins are separated, no obvious peculiar smell is generated after the obtained bletilla striata oligosaccharide is dissolved, no toxic or harmful reagent is added in the extraction process, and the production criterion of health and environmental protection is met.

Description

technical field [0001] The invention relates to the technical field of biological extraction, more specifically, to a bletilla striata oligosaccharide extracted from the rhizome of bletilla striata and a preparation method thereof. Background technique [0002] Bletilla striata is a perennial herb belonging to the family of Orchidaceae. It is also known as white root, ground screw, white chicken baby, horn seven, purple orchid, knife-edge medicine, Lianjicao, etc. The tubers are used for medicine, which can restrain bleeding, reduce swelling and promote granulation; treat diseases such as tuberculosis and hemoptysis, bronchiectasis hemoptysis, gastric ulcer, hematemesis, hematuria and hematochezia. External use to treat traumatic bleeding, burns, chapped hands and feet embolism. It has huge commercial application value in biomedicine, health food, textile printing and dyeing, special coatings and daily chemical industry. [0003] Oligosaccharides, also known as oligosaccha...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07H1/08C07H3/04C07H3/06
CPCC07H1/08C07H3/04C07H3/06C08B37/0003
Inventor 张宝堂郑思佳
Owner HUNAN NUSTREETCARAX
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