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Method for measuring multi-component content of Jingyaokang capsules

A determination method and technology of Jingyaokang are applied in the field of multi-component content determination of Jingyaokang capsule, which can solve the problems of blank detection of various components in Jingyaokang capsule, and it is difficult to reflect the product quality and product as a whole, so as to achieve rapid and efficient determination. , good peak shape, good separation effect

Inactive Publication Date: 2020-04-10
通化卫京药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The current quality standards for Jingyaokang Capsules mainly include the TLC identification of safflower, Xiangjiapi, Fangji and Achyranthes bidentata, and the content measurement of strychnine in Nuxebra chinensis. It is difficult to reflect the quality of the product as a whole or to control the quality of the product as a whole
The patent of invention with the publication number CN108169386A discloses a method for constructing the HPLC characteristic spectrum of Jingyaokang capsules. The obtained HPLC characteristic spectrum can only evaluate the quality of Jingyaokang capsules as a whole
The invention patent with the publication number CN109270203A discloses a construction method of the characteristic map of the active ingredients of Jingyaokang capsules and a quality detection method of Jingyaokang capsules. It only effectively monitors the active ingredients strychnine and / or naringin , but the detection of various components of Jingyaokang Capsules is still blank

Method used

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  • Method for measuring multi-component content of Jingyaokang capsules
  • Method for measuring multi-component content of Jingyaokang capsules
  • Method for measuring multi-component content of Jingyaokang capsules

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A method for assaying the multi-component content of Jingyaokang capsules, comprising the following steps:

[0041] S1. Preparation of mixed reference solution:

[0042] Precisely weigh strychnine, α-eucydine, barley toxin, 11-carbonyl-β-boswellic acid, zedodobicycloenone, hydroxysafflower yellow A, naringin, tetrandrine, and fangchinoline , β-ecdysterone reference substance, placed in the same 25mL measuring bottle, diluted to the mark with 70% methanol, and shaken up, the mass concentrations of the above 10 components were successively 85.6 μg / mL, 339.4 μg / mL, 56.3 Mixed reference solution of μg / mL, 285.2μg / mL, 48.9μg / mL, 73.6μg / mL, 24.8μg / mL, 36.2μg / mL, 59.3μg / mL, 285.6μg / mL.

[0043] S2. Preparation of the test solution:

[0044] Accurately weigh 0.5g of the contents of Jingyaokang Capsules, add 70% methanol to make up to 50mL, airtight, weigh, ultrasonically treat for 10 minutes, weigh again, make up the lost mass with 70% methanol, shake well, filter, Take the ...

experiment example

[0057] 1. Inspection of extraction conditions

[0058] 1. Investigation of the preparation method of the test solution:

[0059] Experiments compared the extraction effects of different extraction solvents such as acetone, ethanol, methanol in water bath reflux, Soxhlet extraction, ultrasonic extraction, etc. The results showed that the overall effect of methanol ultrasonic extraction was the best. At the same time, the extraction effect of different concentrations of methanol (50%, 70%, 90%) as extraction solvent and different extraction time (5, 10, 20 min) was also investigated. The results showed that ultrasonic extraction with 70% methanol for 10 minutes was more complete, and the operation method was simple, stable and reproducible. Using methanol to ultrasonically extract the samples, the resulting spectrum has a large amount of information, including both fat-soluble components and water-soluble components, reflecting all the information contained in the medicinal mat...

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Abstract

The invention provides a method for determining multi-component content of a Jingyaokang capsule. The method comprises the following steps: S1, preparation of a mixed reference substance solution, precisely weighing strychnine, alpha-cypress alkali, periplocin, 11-carbonyl-beta-frankincense acid, curcuma zedoary bicyclo-enone, hydroxysafflor yellow A, naringin, tetrandrine, fangchinoline and beta-ecdysterone respectively, and dissolving the above materials in a solvent to obtain the mixed reference substance solution for later use; s2, preparation of a test solution, taking the Jingyaokang capsule, adding a solvent for extraction, performing filtering, and taking filtrate to obtain the test solution for later use; s3, content determination, precisely sucking the reference substance solution and the test solution respectively, injecting the solutions into a liquid chromatograph, and performing determining to obtain the content. According to the determination method, the contents of tenchemical components such as strychnine, alpha-jacosinine, periplocin, 11-carbonyl-beta-masticinic acid and naringin in the Jingyaokang capsule are determined, and the quality of the Jingyaokang capsule can be better controlled.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a method for determining the content of multiple components in Jingyaokang capsules. Background technique [0002] The Chinese patent medicine Jingyaokang Capsule is composed of ten herbs such as Nuxebra chinensis, Shenjincao, Xiangjiapi, frankincense, myrrh, and safflower; It is used for fracture congestion, swelling pain, fracture recovery period, arthralgia caused by kidney deficiency and dampness, hyperplastic spondylitis, lumbar disc herniation, etc. [0003] The current quality standards for Jingyaokang Capsules mainly include the TLC identification of safflower, Xiangjiapi, Fangji and Achyranthes bidentata, and the content measurement of strychnine in Nuxebra chinensis. It is difficult to reflect the quality of the product as a whole or to control the quality of the product as a whole. The patent of invention with the publication number CN108169386A discloses a method for ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34
CPCG01N30/02G01N30/06G01N30/34G01N2030/047
Inventor 曾英姿李林昌赵磊武丹
Owner 通化卫京药业股份有限公司
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