Sulfonyl transferase, application and aziridine ring biosynthesis method mediated by sulfonyl transferase

Abstract

The invention relates to sulfonyltransferase, an application and an aziridine ring biosynthesis method mediated by sulfonyltransferase. The method comprises the steps of diluting a substrate, 3'-adenosine-5'-phosphate sulfuric acid and sulfonyltransferase to corresponding concentrations by using a PBS buffer solution, reacting at 25 to 42 DEG C for 20 min to 2 h, and then adding 0.5 mol / L NaOH toadjust the pH value of the system to be 10.0 or above, so as to generate an aziridine cyclic compound. According to the invention, an aziridine three-membered ring is synthesized by biocatalysis of sulfonyltransferase for the first time; the basic reaction conditions for enzymatic synthesis of the aziridine ring are determined, and a set of in vitro catalytic synthesis system of the aziridine ringis established, thus laying a theoretical foundation for further obtaining more aziridine structure derivatives with good patent medicine prospects through a combined biosynthesis mode; and a theoretical basis is provided for green synthesis and preparation of aziridine drug intermediates.

Description

technical field [0001] The invention belongs to the technical field of enzyme-catalyzed biosynthesis, and relates to a biosynthesis method of an aziridine three-membered ring, in particular to a sulfonyl transferase, an application thereof and a method for biosynthesis of an aziridine ring mediated. Background technique [0002] Aziridine ring (Aziridine) is a three-membered ring structure composed of a nitrogen atom and two carbon atoms; its structure is: Due to the compact structure and high ring tension of the three-membered ring, it has strong chemical reactivity and can undergo ring opening with a variety of chemical groups (including: various nucleophilic groups, organolithium reagents and Gilman reagents, etc.) Reaction, substitution reaction and addition reaction, etc., have important uses in the synthesis of pharmaceutical intermediates and other organic synthesis processes. At the same time, Aziridine is also an important functional group of many natural active p...

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Problems solved by technology

[0014] In these synthetic methods, generally speaking, a large amount of strongly acid-alkaline polluted wastewater is produced in the homogeneous production process, and the environmental pollution is serious, and the corrosiveness of strong acid-base to equipment also increases the cost of the process

However, the heterogeneous method has problems such as harsh reaction conditions, many steps, short catalyst service life and high loss rate, which makes the overall economic benefit of aziridine ring synthesis not high.

[0015] Through the search, no patent publications related to the patent application of the present invention have been found

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