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Carrot lactobacillus fermentation lysate and preparation method thereof

A technology of lactic acid bacteria fermentation and lysate, applied in biochemical equipment and methods, methods based on microorganisms, fermentation, etc., can solve the problem of unsatisfactory antioxidant activity and achieve biological activity retention, small particle size, and loss reduction Effect

Inactive Publication Date: 2020-04-10
GUANGZHOU ADDITIVE FREE COSMETIC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lysate of lactic acid bacteria fermentation contains lactic acid bacteria, amino acids, small peptides, polysaccharides, B vitamins, lactic acid, sodium lactate and other skin-friendly ingredients, which have antibacterial, calming, repairing, whitening, anti-allergic, anti-inflammatory and moisturizing properties on the skin , adjust the pH value, etc., but the antioxidant activity of the existing lactic acid bacteria fermentation lysate is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Follow the steps below to prepare the lactic acid bacteria carrot lysate:

[0033] (1) Add fresh carrots into water according to the mass ratio of 1:50, stir and pulverize, filter to obtain the filter residue and supernatant, discard the filter residue, and take the supernatant as carrot juice;

[0034] (2) Glucose 50g, peptone 20g, yeast powder 5g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, diammonium hydrogen citrate 2g, magnesium sulfate heptahydrate 0.63g, manganese sulfate monohydrate 0.2g, Tween-80 1g Mix, add carrot juice obtained in step (1) to 1000ml, mix evenly to obtain fermentation medium;

[0035] (3) Add Lactobacillus plantarum into MRS broth and culture at 37°C for 24 hours to obtain seed liquid;

[0036] (4) Add the seed liquid obtained in step (3) to the fermentation medium obtained in step (2), place it in a fermenter for 24 hours at 35°C to ferment and cultivate to obtain a fermented liquid, the initial pH of the fermented culture is 6, an...

Embodiment 2

[0039] Follow the steps below to prepare the lactic acid bacteria carrot lysate:

[0040] (1) Add fresh carrots into water according to the mass ratio of 1:1, stir and pulverize, filter to obtain the filter residue and supernatant, discard the filter residue, and take the supernatant as carrot juice;

[0041] (2) Glucose 48g, peptone 22g, yeast powder 6g, sodium acetate 6g, dipotassium hydrogen phosphate 3g, diammonium hydrogen citrate 1g, magnesium sulfate heptahydrate 0.5g, manganese sulfate monohydrate 0.3g, Tween-80 0.5 g to mix, add the carrot juice obtained in step (1) to 1000ml, mix evenly to obtain the fermentation medium;

[0042] (3) Add Lactobacillus casei into MRS broth and culture at 37°C for 24 hours to obtain seed liquid;

[0043] (4) Add the seed liquid obtained in step (3) to the fermentation medium obtained in step (2), place it in a fermenter for 48 hours at 30°C to ferment and cultivate to obtain a fermented liquid, the initial pH of the fermented culture ...

Embodiment 3

[0046] Follow the steps below to prepare the lactic acid bacteria carrot lysate:

[0047] (1) Add fresh carrots into water according to the mass ratio of 1:90, stir and pulverize, filter to obtain the filter residue and supernatant, discard the filter residue, and take the supernatant as carrot juice;

[0048] (2) Glucose 52g, peptone 18g, yeast powder 4g, sodium acetate 4g, dipotassium hydrogen phosphate 1g, diammonium hydrogen citrate 3g, magnesium sulfate heptahydrate 0.7g, manganese sulfate monohydrate 0.1g, Tween-80 1.5 g to mix, add the carrot juice obtained in step (1) to 1000ml, mix evenly to obtain the fermentation medium;

[0049] (3) Add Lactobacillus bulgaricus to M17 broth and culture at 37°C for 24 hours to obtain seed liquid;

[0050] (4) Add the seed solution obtained in step (3) to the fermentation medium obtained in step (2), place it in a fermenter for 8 hours at 37°C to ferment and cultivate to obtain a fermented liquid, the initial pH of the fermented cul...

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PUM

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Abstract

The invention provides a carrot lactobacillus fermentation lysate, which is prepared by the following steps: (1) adding fresh carrots into water, stirring and crushing, and filtering to obtain a supernatant which is the carrot juice; (2) mixing glucose, peptone, yeast powder, sodium acetate, dipotassium phosphate, diammonium hydrogen citrate, magnesium sulfate heptahydrate, manganese sulfate monohydrate and tween-80, adding the carrot juice, and uniformly mixing the materials to obtain a fermentation culture medium; (3) adding lactic acid bacteria into a seed culture medium, and culturing at 37 DEG C for 24 hours to obtain a seed solution; (4) inoculating the seed solution into the fermentation culture medium, and carrying out fermentation culture to obtain a fermentation solution; and (5)carrying out centrifugal separation on the fermentation liquor to obtain bacterial sludge, adding the bacterial sludge into an xanthan gum aqueous solution, stirring until the material is uniformly dispersed, and carrying out ultrahigh-pressure homogenization treatment to obtain the carrot lactobacillus fermentation lysate. The invention also provides a preparation method of the carrot lactobacillus fermentation lysate. The carrot lactobacillus fermentation lysate provided by the invention has relatively strong antioxidant activity.

Description

technical field [0001] The invention relates to a carrot lactic acid bacteria fermentation lysate and a preparation method thereof. Background technique [0002] Lactic acid bacteria fermentation lysate is a lysate produced by the fermentation of lactic acid bacteria, a type of fungus. The fermentation process can separate many chemical components from the plant substrate to which they were originally attached, increasing their bioavailability , using fermentation technology, according to the principle of fermentation, various phytochemical components can be separated and concentrated. The lysate of lactic acid bacteria fermentation contains lactic acid bacteria, amino acids, small peptides, polysaccharides, B vitamins, lactic acid, sodium lactate and other skin-friendly ingredients, which have antibacterial, calming, repairing, whitening, anti-allergic, anti-inflammatory and moisturizing properties on the skin , adjusting the pH value, etc., but the antioxidant activity of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/99A61Q19/08A61L2/02C12P1/04C12N1/20C12R1/25C12R1/245C12R1/225
CPCA61K8/99A61K2800/85A61L2/02A61Q19/08A61K8/9789C12N1/20C12P1/04
Inventor 喻君孙杨吴泽进李洪金
Owner GUANGZHOU ADDITIVE FREE COSMETIC CO LTD
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