Method for quantitatively detecting bisphenol A

A quantitative detection, bisphenol technology, applied in the field of environmental analytical chemistry, to achieve the effect of fast speed, simple method and good selectivity

Inactive Publication Date: 2020-04-07
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present inventor's new technology allows for quicker analysis than current techniques that require complicated procedures or expensive equipment. It can detect small amounts of substances called baphenic acid (BP) without being affected by other compounds found naturally occurring within our bodies.

Problems solved by technology

Technologies described in this patents involve developing new ways to analyze biological molecules like bisphenols without causing environmental concerns or requiring expensive equipment. There currently exist various techniques for identifying and measuring small amounts of them but they still pose challenges when applied to larger samples containing billions of pounds per day.

Method used

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  • Method for quantitatively detecting bisphenol A

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Experimental program
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Effect test

Embodiment 1

[0025] Graphene dispersion used in the present invention is purchased from Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, product number: TNWRGO, graphene thickness is 0.55 ~ 3.74nm, microchip size is about 0.5 ~ 3 μm, and total oxygen content is at 3% ~ 5%. about.

[0026] Add 15 μL of 0.4 wt% graphene dispersion to a 10 mL serum bottle, then add 100 μL of 6×10 -4 The rhodamine B aqueous solution of M was made to volume with Tris-HCl buffer solution with a pH value of 7.4, and the solution was left for 60 minutes before use. Graphene adsorbs rhodamine B to quench rhodamine fluorescence, and the fluorescence of the test system shows a "turn-off" state at this time.

Embodiment 2

[0028] Add 60 μL of 100 μM bisphenol A, phenol, hydroquinone, tribromophenol, m-aminophenol, 2,4,6-trinitrophenol to the solution prepared in Example 1, and the excitation wavelength is 554 nm The fluorescence of each sample was tested under the following conditions, and it was found that the fluorescence intensity of the sample added with bisphenol A increased significantly, and the fluorescence of the test system showed a "turn-on" state at this time. However, the fluorescence of other phenolic compound samples did not change significantly. At the same time, it can be seen with the naked eye that the solution color of the bisphenol A sample turns pink, while the color of other samples has no obvious change. It shows that the detection method has good selectivity to bisphenol A.

Embodiment 3

[0030] Add 10 μL, 20 μL, 30 μL, 40 μL, 60 μL, 70 μL, 80 μL of 100 μM bisphenol A to the solution prepared in Example 1, so that the final concentrations of bisphenol A in the sample are 100 nM, 200 nM, 300 nM, 400 nM, 600 nM, 700nM, 800nM, under the condition of excitation wavelength of 554 nm, the fluorescence spectrum was measured, and it was found that there was a linear relationship between the fluorescence intensity of the sample and the concentration of bisphenol A, and the linear range was 100-800 nM, see figure 1 . pass figure 1 Fluorescence emission spectra and linear ranges of the graphene-rhodamine system for different concentrations of bisphenol A (the corresponding bisphenol A concentrations from bottom to top of the spectral curve are: 100nM, 200nM, 300nM, 400nM, 600nM, 700nM, 800nM) , the bisphenol A concentration of the sample to be tested can be obtained.

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Abstract

The invention discloses a method for quantitatively detecting bisphenol A, belongs to the field of environmental analytical chemistry, and particularly relates to a method for detecting bisphenol A bya graphene-rhodamine binary system. The method comprises the following steps: firstly, adding rhodamine B into a graphene solution to cause fluorescence quenching of rhodamine B; then adding bisphenol A into the system, and due to the fact that the binding force of bisphenol A and graphene is high, competitive adsorption is conducted on bisphenol A and rhodamine B, rhodamine B is desorbed from the surface of graphene, and fluorescence is recovered; and quantitatively detecting the bisphenol A by measuring the fluorescence change of the system. The method has the advantages of being simple, high in speed, good in selectivity, wide in linear range and the like.

Description

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Claims

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Application Information

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Owner FUJIAN NORMAL UNIV
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