Method for obtaining aseptic tissue culture seedlings of carex
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A technology for tissue culture seedlings and Carex, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high germination rate, low germination rate, and low infection probability, and achieve increased survival rate and reproductive rate Effect
Inactive Publication Date: 2020-04-07
BEIJING INST OF LANDSCAPE ARCHITECTURE
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[0004] The purpose of the present invention is to provide a method for obtaining aseptic tissue-cultured seedlings of Carex, which has the advantages of low infection rate and high germination rate, and solves the problem that Carex seeds are easy to infect and have low germination rate
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Embodiment 1
[0030] S1, collecting explants: choose healthy and excellent plants, the priority selection order is young panicle embryo, stem tip, lateral bud, young root, the height is controlled at 3 mm to 6 mm, and the top of the plant is removed with a sterilized scalpel leaf tip;
[0031] S2, Disinfection: Rinse the wiped implant with clean water for 60-80 minutes, pre-water culture in water containing antibiotics for 2-3 days, and then use 70% ethanol to disinfect for 2 minutes, 5% sodium hypochlorite for 20 minutes, and then use Rinse with sterile water 3-5 times;
[0032] S3, such as image 3 As shown, carry out antibacterial culture after complete disinfection: inoculate the rinsed implants into the culture medium with a concentration of 250mg / L carbenicillin or cephalosporin, and add 25mg / L to the medium respectively Cephaloxime in ammonia, tetracycline at a concentration of 25 mg / L, rifampicin at a concentration of 6 mg / L, polymyxin B at a concentration of 6 mg / L, and 6-BA at a...
Embodiment 2
[0037] S1, collecting explants: choose healthy and excellent plants, the priority selection order is young panicle embryo, stem tip, lateral bud, young root, the height is controlled at 3 mm to 6 mm, and the top of the plant is removed with a sterilized scalpel leaf tip;
[0038] S2, Disinfection: Rinse the wiped implant with clean water for 60-80 minutes, pre-culture it in water containing antibiotics for 2-3 days, then use 70% ethanol to disinfect for 2 minutes, sterilize with 1‰ of mercury for 10 minutes, and then Rinse 3-5 times with sterile water;
[0039] S3, antibacterial culture: inoculate the rinsed implants into a culture medium with a concentration of 250 mg / L carbenicillin or cephalosporin, and add thioxime in a concentration of 25 mg / L to the culture medium Cephalosporin, tetracycline at 25 mg / L, rifampin at 6 mg / L, polymyxin B at 6 mg / L, 6-BA at 9-12 mg / L, 20- 30g / L sucrose cultured for 30-40 days and then transferred to the next stage;
[0040] S4, Carex bud ...
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Abstract
The invention discloses a method for obtaining aseptic tissue culture seedlings of carex, and relates to the technical field of culture seedlings of carex. The method for obtaining the aseptic tissueculture seedlings of carex comprises the steps of selecting healthy excellent plants, controlling the height to be 3-6 millimeters, removing top leaf tips of the plants, after pretreatment is conducted, wiping surfaces of the plants with alcohol with a volume percent of 70% or above, flushing the wiped plants with clear water for 20-30 minutes, then flushing the wiped plants with aseptic water for1-3 times, planting the flushed plants in a primary culture medium with each two plants cultured in each bottle to obtain aseptic seedlings of carex, adding 6-BA with a concentration of 9-12 mg/L andsucrose with a concentration of 20-30 g/L into the primary culture medium, after culture is conducted for 30-40 days, turning into the next stage, planting sprouts into a subculture medium, adding nutrients of the primary culture medium into the subculture medium, conducting culture for 20 days until seedlings emerge, transplanting rooting tissue culture seedlings subjected to seedling temperinginto a transplanting matrix, and conducting cultivation for 1-2 months until seedlings emerge. The method for obtaining the aseptic tissue culture seedlings of carex has the advantages of low infection probability and a high germination rate.
Description
technical field [0001] The invention relates to the technical field of carex seedling cultivation, in particular to a method for obtaining aseptic tissue culture seedlings of carex. Background technique [0002] Carex, that is, sedge, is a plant of the genus Carex in the Cyperaceae family. Perennial herbaceous plant with underground rhizomes, clustered or scattered stalks, triangular, linear leaves, rarely lanceolate, triangular nutlets wrapped in fruit capsules, about 2000 species in the world, Carex in my country About 500 species. [0003] The main ornamental parts of Carex plants are leaves and plant types. The colors and shapes of leaves are rich and colorful. They are ideal lawn grass species and environmental protection plants. They play an important role in restoring and improving the ecological environment. It has high application value in landscaping and sports field lawn construction, but because Carex is mainly explants close to the ground, it is easy to contact...
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