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Polypeptide drug exosome nano-loading system targeting cell membrane and preparation method thereof

A technology of nano-drug loading and exosomes, which is applied in the fields of nano-materials and genetic engineering, can solve problems such as the inability to improve the treatment effect of tumors, and achieve the effects of improving the treatment effect, enhancing the activation effect, and increasing the drug concentration

Active Publication Date: 2021-09-28
饶磊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But usually, the receptors of various cytokines are distributed on the cell surface, and most of the cytokines are delivered into the cells, which cannot improve the therapeutic effect of tumors

Method used

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  • Polypeptide drug exosome nano-loading system targeting cell membrane and preparation method thereof
  • Polypeptide drug exosome nano-loading system targeting cell membrane and preparation method thereof
  • Polypeptide drug exosome nano-loading system targeting cell membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Preparation of Drug System

[0049] First, an exosomeric load polypeptide drug is used, and the load of the proprietary body to the polypeptide drug is completed using the genetic engineering method, and then the outer body of the load polypeptide and the transmigramagnetic nanchromagnetic iron modified, the specific steps are as follows:

[0050] (1) Construct a recombinant plasmid

[0051] With overlapping PCR technology, the tumor necrosis factor TNF-α extracellular sequence (with reverse transcription PCR extraction) is blended with a flexible seven peptide CPP sequence to construct a fusion protein gene; the connection is designed for two pairs of primers. Connecting, TNF-α extracellular sequence sequence using primers F1 and R1 amplification reverse transcriptional cDNA, CPP sequences for amplification, by overlapping PCR technology, using primers F2 and R1 to use TNF-α extracellular gene Connect with the N-terminus of CPP; NHE I and BAMH I bisase digestion,...

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Abstract

The present invention provides a polypeptide drug exosome nano-loading system targeting cell membranes and a preparation method thereof, and constructs a nano-delivery system (CTNF-α-exosome-SPION) with active targeting performance. The fusion polypeptide is distributed to the surface of exosomes through genetic engineering, and SPION is connected to the exosomes through the combination of transferrin and transferrin receptors on the exosomes. With the help of an external magnetic field, the active targeting of SPION leads to a higher local concentration of the loaded drug. In addition, the lipophilicity of the fusion protein, through the delivery of exosomes, binds more to the receptors distributed on the cell surface to enhance the activation activity. The drug-loading system can deliver the polypeptide drug to the location of the polypeptide receptor on the cell surface of the lesion, thereby improving the targeting and activation activity of the polypeptide drug.

Description

Technical field [0001] The present invention belongs to the nanomaterials and genetic engineering field, and more particularly to an exosomal nano-dried system having a target cell membrane and a preparation method thereof. Background technique [0002] In recent years, the progress of surgical surgery and multi-drug chemotherapy has improved the patient's surgical results and quality of life. However, most clinical anticancer drugs have significant side effects due to toxicity to normal cells and lack of targetedness. The emergence of nano-biomaterials has opened up new prospects for cancer drug treatment. The treatment of synergist of nanomaterials can significantly reduce the dose of anticancer drugs, reducing side effects, and increasing delivery of target drugs. [0003] Tumor necrosis factors were the first cytokines used in cancer treatment, and tumor necrosis factors significantly inhibited tumor angiogenesis and cancer cell proliferation. However, the natural tumor necro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K41/00A61K38/19A61K47/64A61K47/46A61K47/02A61K47/42A61P35/00C12N15/62C12N15/85C12N5/10
CPCA61K9/0009A61K9/5115A61K9/5169A61K9/5176A61K38/00A61K41/00A61P35/00C07K14/525C07K2319/00C07K2319/10C12N15/85
Inventor 饶磊庄满娇
Owner 饶磊
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