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A susceptibility gene detection panel related to skin aging characteristics and its application

A technology for skin aging and susceptibility genes, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. It can solve the problems of cumbersome gene chip method, complicated operation, and long time consumption, and achieve high accuracy. High, high detection efficiency, low cost effect

Active Publication Date: 2022-07-29
陕西九州医学检验有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The gene chip method used in this patent is relatively cumbersome, and it is only tried in the research and development stage, and large-scale commercial transformation cannot be achieved
Invention patent (201610367682.9) discloses a primer and probe for detecting skin water retention. The method used is fluorescent quantitative PCR to detect two single nucleotide polymorphism sites. The detection of genes is not comprehensive, and fluorescent quantitative PCR method is suitable For the detection of about 5 known SNP sites, when the number of sites increases, the operation is complicated and time-consuming
In view of the skin sub-health problem of nearly 70% of Chinese people, but the limitations of the above-mentioned existing detection technology, there is no detection panel for the detection of susceptibility genes related to skin aging symptoms for Asian populations (especially Chinese populations)

Method used

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  • A susceptibility gene detection panel related to skin aging characteristics and its application
  • A susceptibility gene detection panel related to skin aging characteristics and its application
  • A susceptibility gene detection panel related to skin aging characteristics and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0033] 1. Detection scheme of susceptibility genes related to skin aging characteristics

[0034] According to the relationship between human skin aging characteristics and genetic genes, susceptibility genes and SNP sites related to skin aging characteristics were determined (as shown in Table 1), and a detection panel was formed.

[0035] For all SNP sites involved in the detection panel, design multiple PCR amplification primer pairs SEQ ID NO.1-SEQID NO.90 nucleotide sequence, and single base extension primer SEQ ID NO. The nucleotide sequence shown in 113.

[0036] Using human genomic DNA as samples (oral mucosa, peripheral blood, tissue), amplification and extension products were obtained by multiplex PCR amplification technology.

[0037] Matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to perform SNP genotyping of samples, to evaluate individual skin conditions from a genetic perspective, and to provide a scientific basis for preci...

Embodiment 2

[0040] Example 2 Sample extraction and analysis

[0041] In order to verify the accuracy and validity of the primers for the detection panel of the present invention for detecting susceptibility genes related to skin aging characterization, 2 samples were selected, numbered RY-BS-1 and RY-BS-2 respectively, and each sample was set Three duplicate wells were used for SNP typing on the nucleic acid mass spectrometry platform. The process is as follows:

[0042] A. Oral mucosal DNA extraction: DNA extraction from oral mucosal exfoliated cells was carried out using Hangzhou Biori oral swab genomic DNA extraction kit. Add a certain amount of absolute ethanol to WB1 and WB reagents. Take out the sampling cotton swab from the sampling tube and transfer it to a 2ml centrifuge tube, cut the cotton swab from its stem with scissors; add 600uL Lysis Buffer and 10uL PKSolution, shake and mix for 15 seconds; incubate at 56°C for 15 minutes; Add 300uL of absolute ethanol to the centrifuge ...

Embodiment 3

[0048] Example 3 Analysis of gene detection results

[0049] SNP genotyping was performed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and the detection results were output by TYPER4.0 software. Table 2 and Table 3 are respectively the SNP site gene detection results of Examples RY-BS-1 and RY-BS-2. It can be seen from Table 2 and Table 3 that the genotypes of the 45 sites of the two examples are all It can be detected accurately, and the detection rate is 100%; after the comparison of 3 genotypes in all loci, the coincidence rate reaches 100%, and 3 of the 2 loci of the RY-BS-1 sample of Example RY-BS-1 are randomly selected. The multiple-hole mass spectrum is shown and explained in the accompanying drawings, see the accompanying drawings for details.

[0050] The SNP site gene detection result of table 2 embodiment sample RY-BS-1

[0051] SNP site Test results SNP site Test results SNP site Test results rs1001179 ...

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Abstract

The invention belongs to the field of gene detection, and in particular relates to a susceptibility gene detection panel related to skin aging characteristics and its application. The detection panel is used to detect 33 skin aging characteristics related susceptibility genes of human genomic DNA. The panel includes multiplex PCR amplification primers and single base extension primers for 45 SNP sites. Compared with the prior art, the present invention covers a large number of susceptibility gene loci related to skin aging symptoms, and is specifically aimed at the skin gene detection of Asian populations (especially Chinese populations), which can more efficiently and scientifically evaluate individual skin conditions, and can effectively evaluate the skin condition of individuals. Analyze the root causes of skin problems, and provide scientific basis for accurate skin care and personalized skin care products customization for Asian people (especially Chinese people).

Description

technical field [0001] The invention belongs to the field of gene detection, and particularly relates to a susceptibility gene detection panel related to skin aging characteristics and its application. Background technique [0002] Jay Tessman, Ph.D., Chief Scientist at P&G Beauty, said: "Through the Human Genome Project, we can analyze the hundreds of genetic changes that occur during aging. The skin continues to age through eight different methods, each of which Controlled by its own genome. Whether you're slowly aging like Cliff Richard or wrinkled like Keith Richards, it's determined by your lifestyle , and are partly played by these genes." [0003] Skin aging is caused by a variety of complex causes, which can be summarized into two categories: internal and external. External factors are common such as long-term ultraviolet radiation, harsh climate and environment, bad living habits and excessive work pressure; internal factors are genetic factors. Scientific resear...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 张煜婷聂苏秦俞瑞卿张颖
Owner 陕西九州医学检验有限公司
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