Efficient green manufacturing process of amino acid

A manufacturing process and green manufacturing technology, applied in microorganism-based methods, microorganisms, bacteria, etc., can solve the problems of large amount of acid and alkali, high Baume degree and viscosity of waste liquid, environmental pollution, etc., and reduce the amount of acid and alkali used. and water consumption, reduce Baume degree and viscosity, and improve the effect of greening level

Active Publication Date: 2020-03-24
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The glutamic acid extraction process mainly has the following disadvantages: 1. The existing domestic amino acid production still uses traditional technical means such as plate and frame, separation, decolorization, and drying. The product has low purity, large amount of acid and alkali, and serious environmental pollution. Key factors for the green development of the fermentation industry
2. Traditional flocculants cause high Baume degree and viscosity of waste liquid, which causes difficult technical problems in wastewater treatment
3. During the drying process, the material liquid is prone to coking, which affects the quality of the product and produces bad smell

Method used

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  • Efficient green manufacturing process of amino acid
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  • Efficient green manufacturing process of amino acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] An efficient green manufacturing process for amino acids, which includes the following steps:

[0041] 1) An optimized glutamic acid fermentation medium, which includes fermentation medium A and fermentation medium B; the fermentation medium A is added first, and then the fermentation medium B is added at intervals of 24 hours.

[0042] The preparation method of described fermentation medium A is: get each raw material: glucose 80g / L, yeast extract 20g / L, K 2 HPO 4 2g / L, MgSO 4 ·7H 2 O 50mg / L, 2-hydroxyethylamine 40mg / L, CeCl 3 10mg / L, MnSO 4 ·H 2 O 3mg / L, FeSO 4 ·7H 2 O 3mg / L, VB 1 10mg / L, biotin 7μg / L; After mixing all raw materials evenly, adjust the pH to 6.5, sterilize at 121°C for 15min, and cool naturally to prepare fermentation medium A;

[0043] The preparation method of the fermentation medium B is as follows: take each raw material: succinic acid 5g / L, urea 2g / L, chitosan 80mg / L; after stirring each raw material evenly, adjust the pH to 6.5, and ster...

Embodiment 2

[0049] An efficient green manufacturing process for amino acids, which includes the following steps:

[0050] 1) An optimized glutamic acid fermentation medium, which includes fermentation medium A and fermentation medium B; the fermentation medium A is added first, and then the fermentation medium B is added at intervals of 24 hours.

[0051] The preparation method of described fermentation medium A is: get each raw material: glucose 100g / L, yeast extract 25g / L, K 2 HPO 4 1g / L, MgSO 4 ·7H 2 O 70mg / L, 2-hydroxyethylamine 20mg / L, CeCl 3 5mg / L, MnSO 4 ·H 2 O 2mg / L, FeSO 4 ·7H 2 O 2mg / L, VB 1 5mg / L, biotin 5μg / L; After mixing all raw materials evenly, adjust the pH to 6.5, sterilize at 121°C for 15min, and cool naturally to prepare fermentation medium A;

[0052] The preparation method of the fermentation medium B is as follows: take each raw material: succinic acid 7g / L, urea 2g / L, chitosan 50mg / L; after stirring each raw material evenly, adjust the pH to 6.5, and ster...

Embodiment 3

[0057] 1. CeCl 3 Effects of rare earth salts on cell concentration, glutamic acid content and sugar-acid conversion rate.

[0058] The fermentation medium is: glucose 80g / L, yeast extract 20g / L, K 2 HPO 4 2g / L, MgSO 4 ·7H 2 O 50mg / L, CeCl 3 0-40mg / L, MnSO 4 ·H 2 O 3mg / L, FeSO 4 ·7H 2 O 3mg / L, VB 1 10mg / L, biotin 7μg / L;

[0059] The fermentation process is the same as in Example 1, and the 100L fermenter contains 70L fermentation medium.

[0060] Set CeCl 3 The added concentration is 0, 2.5, 5, 10, 20, 40mg / L, such as Figure 1-2 As shown, with CeCl 3 The concentration of bacteria and glutamic acid content increased with the increase of the amount of addition. When the amount of addition was 10mg / L, the concentration of bacteria and glutamic acid content reached the peak value, and then showed a downward trend, but during the whole process, sugar-acid The conversion did not change significantly (figure not shown); indicating that CeCl 3 Rare earth salts can pro...

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Abstract

The invention belongs to the technical field of amino acid production, and discloses an efficient green manufacturing process of amino acid. The process comprises the steps of step 1) preparing an optimized fermentation culture medium; step 2) performing a fermentation process; and step 3) performing an extraction separation process. According to the invention, the fermentation efficiency is high;the separation and extraction process is improved; the product recovery rate and purity are improved; the energy consumption and sewage discharge are reduced; and the requirements of green manufacturing are met.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and in particular relates to an efficient green manufacturing process of amino acid. Background technique [0002] Amino acids have played an increasingly widespread role in the food industry, medicine, agriculture, animal husbandry, and human health, health care, and cosmetics industries. According to statistics, the types of amino acids and their derivatives have grown from about 50 in the 1960s to more than 1,000 now. At present, the world's amino acids have reached more than 6 million tons, and the production has been expanded and transferred to developing countries. According to a survey report released by the International Amino Acid Science Association, the Asia-Pacific region has become the world's largest amino acid market. my country is a big country in the production and consumption of amino acids. It ranks among the top in the world both in terms of total industrial ou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/18C12N1/20C12N1/38C12R1/13
CPCC12P13/18C12N1/20C12N1/38
Inventor 季新利杨雪王小平刘元涛李树标
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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