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Bifidobacterium pseudocatenatum and application thereof

A bifidobacterium, chain-like technology, applied in the field of pseudo-chain bifidobacteria, can solve the problem of limited number of probiotics and achieve the effect of weight loss

Active Publication Date: 2020-03-20
毕德玺
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of known probiotics is still limited, and the intestinal microecological resources and related functions still need to be deeply excavated and explored.

Method used

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  • Bifidobacterium pseudocatenatum and application thereof
  • Bifidobacterium pseudocatenatum and application thereof
  • Bifidobacterium pseudocatenatum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] This example is the isolation and strain identification of pseudostrand bifidobacterium DX1.

[0036] (1) Isolation of Pseudostrand Bifidobacterium DX1:

[0037] Bifidobacterium pseudostrandum DX1 ​​was isolated from the intestinal epithelium of the human intestine. The tissue was put into tryptone soybean broth (TSB, prepared from finished TSB dry powder), mechanically crushed, and diluted 10 2 -10 3 times, spread on Columbia blood plate (prepared from finished Columbia agar base dry powder, 5-7% sterile defibrated sheep blood), and culture anaerobically at 37°C for 2-3 days. Pick the suspected single colony growing on the plate and identify the bacterial species. Finally a DX1 isolate was obtained. The obtained DX1 single colony was streaked and cultured again 2-3 times on the Columbia blood plate to purify the strain. The isolated Pseudostrandum bifidobacteria were cultured in Columbia blood agar medium under anaerobic culture at 37°C. Such as figure 1 and f...

Embodiment 2

[0042] This example is the verification of the probiotic efficacy of Bifidobacterium pseudostrandum DX1.

[0043] Twenty-five four-week-old C57BL / 6J mice of SPF (specific pathogen free) grade were given low-fat diet (10kcal% fat) to adapt to feeding for four weeks; after that, they were randomly divided into three groups, namely low-fat diet group (LFD, n=7), High-fat diet group (HFD, n=9) and high-fat diet plus DX1 strain gavage group (HFD+Bp, n=9). Among them, the LFD group continued to receive low-fat feed (10kcal%Fat); the HFD group and HFD+Bp group both received high-fat feed (60kcal%Fat); in addition, the HFD+Bp group received B.pseudocatenulatum once every two days The DX1 strain was administered orally, and the dose was 1×10 each time. 9 CFU, while the HFD group received normal saline gavage correspondingly. This continued for 16 weeks.

[0044] (1) DX1 strain significantly relieves obesity caused by high-fat diet;

[0045] During the experiment, the weight changes...

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Abstract

The invention discloses Bifidobacterium pseudocatenatum and application thereof. The strain is separated from human intestinal tract for the first time, the sequence of 16S rRNA gene of the strain isshown as SEQ ID No.1, the strain is named DX1, and the preservation number is CCTCC NO: M 2019852. A mouse model proves that the DX1 strain has probiotic effects of relieving obesity, abnormal blood glucose homeostasis and liver steatosis; the DX1 strain can obviously reduce the weight, improve glucose tolerance and insulin resistance and improve liver steatosis in a high-fat diet mouse, does notcause systemic inflammation and intestinal inflammation, has certain safety, and lays a certain theoretical foundation for preventing or treating metabolic diseases such as obesity and diabetes.

Description

technical field [0001] The invention relates to the technical field of bifidobacteria, in particular to a strain of bifidobacterium pseudocatenulatum (Bifidobacterium pseudocatenulatum), which is isolated from human intestinal tract, and application thereof. Background technique [0002] Metabolic diseases such as obesity and diabetes have become global health problems. The latest statistics show that the obese population continues to grow, and by 2016 the number of overweight adults and children worldwide (BMI ≥ 25kg / m 2 ) population proportions have been as high as 40% and 18% respectively, among which adult obesity (BMI≥30kg / m 2 ) population accounted for about 12% of men and about 16% of women, four times and double those in 1975, respectively. At the same time, the incidence of diabetes is also on the rise. According to the statistics of the World Health Organization, in 2014, there were more than 420 million diabetics worldwide, and the incidence rate among adults ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A61P3/04A61P1/16A61P3/10C12R1/01
CPCC12N1/20A61K35/745A61P3/04A61P1/16A61P3/10C12R2001/01C12N1/205
Inventor 毕德玺
Owner 毕德玺
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