Fluorescence microscopic illumination method adopting deep ultraviolet light source

A technology of deep ultraviolet light source and lighting method, which is applied in the field of fluorescent microscopic excitation lighting, can solve the problems of high cost and need for color filter blocks, and achieve the effects of low cost, simple structure and avoiding light energy loss

Pending Publication Date: 2020-03-17
MOTIC CHINA GRP CO LTD
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, this comparison file is used for transmitted fluorescence, which requires a color filter block, and its cost is also high

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  • Fluorescence microscopic illumination method adopting deep ultraviolet light source
  • Fluorescence microscopic illumination method adopting deep ultraviolet light source
  • Fluorescence microscopic illumination method adopting deep ultraviolet light source

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Embodiment Construction

[0020] To further illustrate the various embodiments, the present invention is provided with accompanying drawings. These drawings are a part of the disclosure of the present invention, which are mainly used to illustrate the embodiments, and can be combined with related descriptions in the specification to explain the operating principles of the embodiments. With reference to these contents, those skilled in the art should understand other possible implementations and advantages of the present invention. Components in the figures are not drawn to scale, and similar component symbols are generally used to denote similar components.

[0021] like figure 1 , 2 , 3, shown in 4, the present invention provides a kind of fluorescence microscope illumination method that adopts deep ultraviolet light source, comprises 1, specimen, 2, off-axis paraboloid, 3, light source, 4, objective lens, 5, eyepiece, 6, lens array , 7, head, 8, dark field objective lens, 9, object stage, 10, fram...

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Abstract

The invention discloses a fluorescence microscopic illumination method adopting a deep ultraviolet light source. The system is composed of an ocular lens, an impermeable deep ultraviolet-impermeable objective lens, a deep ultraviolet excitation light source and a light condensation system. The deep ultraviolet excitation light source and the light condensation system are installed around the objective lens to form dark field illumination irradiating a specimen; the specimen is excited to generate fluorescence; the objective lens collects the fluorescence and then imaging on the ocular lens isperformed. According to the invention, with the short-working-distance objective lens, the light energy loss is avoided, the optical efficiency is improved, color filtering blocks are not needed, thestructure is simplified, and the cost is lowered.

Description

technical field [0001] The invention relates to the field of microscope technology, in particular to a fluorescent microscopic excitation lighting technology. Background technique [0002] Fluorescence microscopy has the advantages of high contrast, high detection ability, less stimulation to cells, and multiple staining. It can be widely used in biology, hematology, histology, pathology, medicinal chemistry, and clinical trials. A fluorescence microscope is mainly composed of a fluorescence excitation source, a light concentrating system, a color filter block (composed of an excitation filter, a spectroscopic color separation filter, and a blocking filter) and a microscope. Generally, an epi-illumination structure is used. The specific principle is: fluorescence excitation After the source is powered on, the light emitted by the source passes through the condensing, excitation color filter (can only transmit light less than a certain wavelength) and spectroscopic color sepa...

Claims

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Application Information

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IPC IPC(8): G02B21/16
CPCG02B21/16
Inventor 陈木旺林顺华林茂英
Owner MOTIC CHINA GRP CO LTD
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