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Germ cell dedifferentiation culture medium and preparation method thereof

A technology for culturing substrates and germ cells, applied in the field of germ cells, can solve the problems of lack of antibacterial and sterilization ability, affecting the dedifferentiation effect of germ cells, etc., and achieve the effect of reducing the effect, enhancing the antibacterial, and improving the antibacterial environment.

Inactive Publication Date: 2020-02-28
顾霆
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Soybean germ cells need to use culture medium during the dedifferentiation process. Although the existing culture medium can provide nutrients during the dedifferentiation process of germ cells, they do not have antibacterial and sterilizing capabilities, which may easily affect the dedifferentiation of germ cells. Effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A germ cell dedifferentiation culture medium of the present embodiment, the formula of the germ cell dedifferentiation culture medium is: MS, modified agar 6g / L, glucose 20g / L, 2,4-dichlorophenoxyacetic acid 0.5mg / L, fructose 0.5mg / L, nutrient solution 0.mg / L, modified montmorillonite 5g / L, pH value is 5.6.

[0025] The preparation method of the modified agar of the present embodiment is as follows: the agar is first irradiated with ultraviolet rays for 10 minutes, and then blended with the sodium molybdate solution for 10 minutes, and the blending speed is 200 r / min. After the blending is completed, filter, and then dry, that is Can.

[0026] The power of the ultraviolet irradiation in this embodiment is 20W.

[0027] The nutrient solution of this embodiment includes the following raw materials in parts by weight: 1.2 parts of magnesium sulfate, 1.1 parts of sodium sulfate, 0.8 parts of zinc sulfate, 0.8 parts of copper sulfate, and 0.2 parts of Chinese herbal medici...

Embodiment 2

[0036] A germ cell dedifferentiation culture medium of this embodiment, the formula of the germ cell dedifferentiation culture medium is: MS, modified agar 8g / L, glucose 26g / L, 2,4-dichlorophenoxyacetic acid 1.1mg / L, fructose 1.1mg / L, nutrient solution 0.7mg / L, modified montmorillonite 15g / L, pH value 5.8.

[0037] The preparation method of the modified agar of the present embodiment is as follows: the agar is first irradiated with ultraviolet light for 20min, then blended with the sodium molybdate solution for 20min, the blending speed is 280r / min, after the blending is completed, filter, and then dry again, namely Can.

[0038] The power of the ultraviolet irradiation in this embodiment is 100W.

[0039] The nutrient solution of this embodiment includes the following raw materials in parts by weight: 1.3 parts of magnesium sulfate, 1.2 parts of sodium sulfate, 1.0 part of zinc sulfate, 0.9 parts of copper sulfate, and 0.5 parts of Chinese herbal medicine starter.

[0040]...

Embodiment 3

[0048] A germ cell dedifferentiation culture medium of the present embodiment, the formula of the germ cell dedifferentiation culture medium is: MS, modified agar 7g / L, glucose 23g / L, 2,4-dichlorophenoxyacetic acid 0.7mg / L, fructose 0.8mg / L, nutrient solution 0.35mg / L, modified montmorillonite 10g / L, pH value is 5.7.

[0049] The preparation method of the modified agar of the present embodiment is as follows: the agar is first irradiated with ultraviolet rays for 15 minutes, then blended with the sodium molybdate solution for 15 minutes, the blending speed is 240r / min, after the blending is completed, filter, and then dry, that is Can.

[0050] The power of the ultraviolet irradiation in this embodiment is 60W.

[0051] The nutrient solution of this embodiment includes the following raw materials in parts by weight: 1.25 parts of magnesium sulfate, 1.15 parts of sodium sulfate, 0.9 parts of zinc sulfate, 0.85 parts of copper sulfate, and 0.35 parts of Chinese herbal medicine...

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PUM

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Abstract

The invention discloses a germ cell dedifferentiation culture medium. The formula of the germ cell dedifferentiation culture medium comprises MS, 6-8 g / L of modified agar, 20-26 g / L of glucose, 0.5-1.1 mg / L of 2, 4-dichlorophenoxyacetic acid, 0.5-1.1 mg / L of fructose, 0.2-0.7 mg / L of a nutrient solution and 5-15 g / L of modified montmorillonite, and the pH value is 5.6-5.8. According to the germ cell dedifferentiation culture medium disclosed by the invention, agar is modified on the conventional basis; after agar is modified by a sodium molybdate solution, the nutrient medium energy of the germ cell dedifferentiation culture medium can be enhanced; meanwhile, the antibacterial environment of the medium can be improved, the nutrient solution is added to enable the culture medium to providevarious trace elements, so that the differentiation degree of germ cells is improved; and the Chinese herbal medicine leavening agent is added in the form of a nutritional agent, so that plants can becontinuously in a sterilized environment when in nutrient absorbing process.

Description

technical field [0001] The invention relates to the technical field of germ cells, in particular to a germ cell dedifferentiation culture substrate and a preparation method thereof. Background technique [0002] Soybean is one of the important food crops in China. It has been cultivated for 5,000 years. It was called Shu in ancient times. It is mainly produced in Northeast China. It is a crop whose seeds are rich in plant protein. Soybeans are most commonly used to make various soy products, extract soybean oil, brew soy sauce and extract protein. Okara, or soybeans ground into a meal, are also commonly used in livestock feed. Beans are commonly known as soybeans. Fabaceae soybean is an annual herb, 30-90 cm high. Soybean pods are hypertrophic, slightly curved, drooping, yellow-green, densely covered with brown-yellow long hairs; 2-5 seeds, oval, nearly spherical, smooth seed coat, various in light green, yellow, brown and black. The flowering period is from June to July...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
CPCC05D5/00C05G3/00C05D9/00C05D9/02C05F11/00C05F11/08
Inventor 顾霆
Owner 顾霆
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