Cudrania tricuspidata breeding method using hard branch cuttage
A technology of hard branch cutting and zhe wood, applied in the breeding field of zhe wood hard branch cutting, can solve problems such as limited resources and the like
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Embodiment 1
[0027] Material: annual Zhe wood branches;
[0028] Treatment: Soak with 200mg / L IBA solution (i.e. indole butyric acid solution) for 8 hours;
[0029] Protocol: Three biological replicates, 50 cuttings per replicate;
[0030] Specific operation:
[0031] 1. Take the vigorous annual Zhe wood branches, remove all the leaves on the branches, and cut the branches into 8 long sections;
[0032] 2. Bundle 50 stems cut into small sections and tie them with rubber bands;
[0033] 3. Prepare 1L of 200mg / L IBA solution;
[0034] 4. Soak the pruned branches in 200mg / L IBA solution for 8 hours;
[0035] 5. Prepare the matrix for cuttings, mix perlite: vermiculite: peat according to the volume ratio of 1:1:1, and then put it into a 50-hole seedling tray.
[0036] 6. Insert the soaked branches in step 4 into the prepared seedling trays in step 5, and insert the 2 cm of the base of the branches into the matrix of the trays.
[0037] 7. After the cuttings are placed in a solar greenhou...
Embodiment 2
[0046] Material: annual Zhe wood branches;
[0047] Treatment: Soak in 200mg / L IBA for 8h;
[0048] Protocol: Three biological replicates, 50 cuttings per replicate;
[0049] Cutting substrate: The substrate block was purchased from Pinshi Top Gardening (Shanghai) Co., Ltd. The substrate block is a cylinder with a diameter of 5 cm and a height of 4 cm. The components are peat and coconut peat with a volume ratio of 3:1;
[0050] Specific operation:
[0051] 1. Take the robust annual Zhe wood branches, remove all the leaves on the branches, and cut the branches into 8cm long sections with branch clippers;
[0052] 2. Bundle 50 stems cut into small sections and tie them with rubber bands;
[0053] 3. Prepare 1 liter of 200mg / L IBA solution;
[0054] 4. Soak the pruned branches in 200mg / L IBA solution for 8 hours;
[0055] 5. Put the matrix block into a 50-hole tray;
[0056] 6. Insert the soaked branches in 4 into the prepared seedling trays in 5, and insert the base of th...
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