Nano composite material for detecting silver ions and preparation method of nano composite material

A technology of nanocomposite materials and silver ions, which is applied in the field of nanocomposite materials for detecting silver ions and its preparation, to achieve the effects of high selective detection, excellent selection and increased sensitivity

Pending Publication Date: 2020-02-21
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gold nanoparticles with a hollow and porous structure are used as nanocarriers, and C-C base mismatches are used to pair Ag + Constructing nanocomposites for detection of Ag with unique biorecognition + technology has not yet been reported in the literature

Method used

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  • Nano composite material for detecting silver ions and preparation method of nano composite material

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Embodiment 1

[0020] A method for preparing a nano-gold composite material based on exonuclease cycle amplification technology and base mismatch recognition technology proposed by the present invention, comprising the following steps:

[0021] (1) Design and synthesize Ag + Cytosine-rich nucleic acid biomolecules with a certain base length, which are recognized by base mismatches, and which can interact with Ag + A specific base mismatch recognition reaction occurs;

[0022] (2) Wash mercapto magnetic beads (20 μL) with MOPS buffer solution, then mix them with nano-gold carrier solution (400 μL) with hollow and porous structure, add polydiallyldimethylammonium chloride solution (200 μL, 11.664mg / mL), magnetically separated after 10h at 37°C, and washed with MOPS buffer solution;

[0023] (3) Add rhodamine B solution (2μL, final concentration 1.0×10 -4 mol / L), dilute to 100 μL with MOPS buffer solution with pH=7.0, add identifiable Ag after 10 h at 37 °C + Biomolecule solution (10 μL, fi...

Embodiment 2

[0026] A nano-gold composite material based on exonuclease cycle amplification technology and base mismatch recognition technology proposed by the present invention to detect Ag + The method is as follows:

[0027] (1) Dispense 10 μL containing Ag + The sample solution to be tested is added to the nano-gold composite material based on the exonuclease cycle amplification technology and base mismatch recognition technology proposed by the present invention, diluted to 100 μL with MOPS buffer solution (pH=7.0), and added to the exonuclease Enzyme ExoⅢ(20U), shake at 37°C for 2h, recognize Ag on the surface of hollow and porous gold nanocarrier + biomolecules and Ag + A specific recognition reaction occurs to form C-Ag + The -C base pair undergoes conformational transformation at the same time and breaks away from the surface of the nanocarrier, so that the blocked "pore cap" is opened, and the dye molecule rhodamine B in the nanocarrier is released; at the same time, due to th...

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Abstract

The invention provides a nano-gold composite material based on an exonuclease cyclic amplification technology and a base mismatch recognition technology. The nano composite material can be applied tothe fields of food, environment, medicine and health and the like. According to the nano-gold composite material of the invention, nanogold with a hollow and porous structure is combined with biomolecules capable of identifying Ag<+>, so that a nano composite material with a hole cap can be constructed. When a sample solution containing silver ions is added, the silver ions acts on biomolecules onthe surface of the nano-carrier, so that the biomolecules are separated from the surface of the nano-carrier; dye molecules in the nano-carrier are released; after being separated, a supernate generates fluorescence emission under the irradiation of exciting light with a certain wavelength; and the silver ions are detected according to the intensity of the fluorescence emission signals. Meanwhile, the cyclic amplification of the fluorescence signals is realized by utilizing the shearing action of exonuclease. The method has the advantages of simplicity, high efficiency, high sensitivity, highselectivity, convenience, rapidness, low cost and wide application range.

Description

technical field [0001] The invention relates to a nano-gold composite material based on exonuclease cycle amplification technology and base mismatch recognition technology and a preparation method thereof, in particular to a nano-composite material for detecting silver ions and a preparation method thereof. Background technique [0002] Silver ions as heavy metal ions (Ag + ) and mercury ions (Hg 2+ ) is similar, it is a metal ion with high toxicity, and it is also a widely distributed environmental pollutant. Even at low concentrations, it can cause serious and permanent toxicity to the environment and humans. More seriously, through polluted water sources, Ag + It can enter the human food chain after accumulating in agricultural and aquatic products. If prolonged exposure to Ag + The environment in which it exists may lead to the slow occurrence of human degenerative diseases in the body and nervous system. Therefore, the establishment of efficient, sensitive and eco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/553
CPCG01N33/553G01N33/5308
Inventor 王卫董颖杰钟华万均罗细亮
Owner QINGDAO UNIV OF SCI & TECH
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