Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primer group for detecting streptococcus salivarius, detection system and applications of primer group and detection system

A technology of streptococcus salivarius and primer set, which is applied in the biological field to achieve the effects of simple operation, easy promotion and high accuracy

Inactive Publication Date: 2020-02-18
SUZHOU PRECISION BIOTECH CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

FIT uses monoclonal or polyclonal antibodies to directly detect hemoglobin in human stool samples, which is not affected by food intake. However, the sensitivity of FIT for detecting cancer is 80%, and the sensitivity for detecting highly atypical adenoma is only 20%. %-30%, in order to improve the detection rate, it needs to be repeated

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer group for detecting streptococcus salivarius, detection system and applications of primer group and detection system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 detection sample and bacterial classification

[0038] The strains required for the research are derived from the stool samples of the subjects. The subjects did not take antibiotics or similar drugs within three months before the collection of the stool samples. Refer to the instructions of the Changanyi ® stool collection box to collect the stool samples of the subjects. Store at 2-8°C in the laboratory.

Embodiment 2

[0039] Example 2 Primer Design and Identification

[0040] The microbial 16S rRNA sequence contains 9 variable regions and 10 highly conserved regions. In order to ensure the specificity of the primers, primers were designed in the conserved regions; in addition, compared with other variable regions, the V3-V4 regions can cover more than 98% of the Bacteria, and the length of the amplified product in the V3-V4 region meets the length requirements of qPCR for the expected product of the primer; based on this condition, a pair of primers that meet the requirements are designed to identify the total amount of all microorganisms in the stool sample.

[0041] Download the bacterial 16S rRNA sequence from the NCBI website (https: / / www.ncbi.nlm.nih.gov / nμccore / ) according to the bacterial name, and then use the primer design software Primer Primer 5 to design bacterial-specific primers; qPCR primer design requirements : The primer length is about 18-25bp, avoiding secondary structure...

Embodiment 3

[0045] The assembly of embodiment 3 kits

[0046] Streptococcus salivarius PCR reaction solution: Tris-HCl: 10-75mmol / L, pH8.5; Ammonium sulfate: 10-40mmol / L; MgCl 2 : 1-5.0mmol / L; dNTPs: 100-500μmol / L; final primer concentration: 0.25-1.25ng / μL.

[0047] Total bacteria PCR reaction solution: Tris-HCl: 50mmol / L, pH8.5; Ammonium sulfate: 25mmol / L; MgCl 2 : 2.5mmol / L; dNTPs: 350μmol / L;

[0048] Negative control PCR reaction solution: Tris-HCl: 40mmol / L, pH8.5; Ammonium sulfate: 30mmol / L; MgCl2: 3mmol / L; dNTPs: 300μmol / L;

[0049] Positive control PCR reaction solution: Tris-HCl: 40mmol / L, pH8.5; Ammonium sulfate: 30mmol / L; MgCl 2 : 3mmol / L; dNTPs: 300μmol / L;

[0050] Negative quality control: sterile water;

[0051] Positive quality control: Streptococcus salivarius genomic DNA.

[0052] The structure of the intestinal microecology kit is shown in Table 2 below:

[0053] Table 1 Kit structure

[0054]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a primer group for detecting streptococcus salivarius, a detection system and applications of the primer group and the detection system. The primer group is a specific primer designed for 16S rRNA (ribosomal ribonucleic acid) of intestinal bacteria, and the specific primer comprises 3-5 random bases. The detection system is explored and verified based on the primer group; steps and conditions are mutually matched to achieve synergic enhancement; a detection result of the detection system is stable and accurate; the detection steps are concise and efficient; and wide application prospects and large market values are achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a primer set for detecting Streptococcus salivarius, a detection system and an application thereof. Background technique [0002] Colorectal cancer is the third most common cancer in the world. 1.2 million patients are diagnosed with CRC every year, and 600,000 people die every year. In 2015, the incidence of colorectal cancer in China accounted for 24.3% of the world, and the number of deaths accounted for 22.9% of the world. According to the study At least 80% of colorectal cancers evolve from colorectal adenomas, which lasted about 10-15 years. Active diagnosis and treatment of colorectal adenomas is an important way to control and reduce colorectal cancer. It has been reported that early detection and treatment can make 50% of colorectal cancer. The annual survival rate is as high as 90%. However, in our country, more than 80% of patients have developed to the middle and late stag...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/46
CPCC12Q1/689C12Q2600/106
Inventor 朱永亮穆延召杨敏王一伟
Owner SUZHOU PRECISION BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com