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Sugar-free culture and strong seedling transplanting method for virus-free sweet potato seedlings

A sugar-free culture and virus-free seedling technology, applied in the field of plant tissue culture, can solve the problems of microbial contamination transplanting survival rate, poor growth quality, etc., and achieve the effects of good water retention performance and good air permeability.

Active Publication Date: 2020-02-11
COTTON RES INST SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of poor growth quality of the existing sweet potato virus-free seedlings, serious microbial pollution and low transplanting survival rate, the present invention provides a sugar-free cultivation and transplanting method of the virus-free sweet potato seedlings

Method used

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  • Sugar-free culture and strong seedling transplanting method for virus-free sweet potato seedlings
  • Sugar-free culture and strong seedling transplanting method for virus-free sweet potato seedlings
  • Sugar-free culture and strong seedling transplanting method for virus-free sweet potato seedlings

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Effect test

Embodiment 1

[0032] The present embodiment provides a sugar-free cultivation and transplanting method for strong seedlings of sweet potato virus-free seedlings, comprising the following steps:

[0033] (1) Carry out detoxification tissue culture of sweet potato in a test tube, proliferate the detoxification seedlings, select the detoxification seedlings grown for 45 days, cut off old roots, cut into 4cm stem sections, and transplant into sugar-free culture containing vermiculite In the box, each box contains 670g of vermiculite, poured 800mL of nutrient solution, transplanted 70 seedlings per box, covered the box cover and sealed the gap between the box cover and the box body with parafilm; the nutrient solution contained 2g / L nitric acid Ammonium calcium and 170mg / LKH 2 PO 4 , the rest is deionized water;

[0034] (2) After transplanting, put it on a culture rack in a closed room, and use CO 2 The controller feeds CO into the incubator 2 , CO 2 CO set by the controller 2 The highest...

Embodiment 2

[0043] This embodiment provides a method for transplanting sweet potato virus-free seedlings for sugar-free cultivation of strong seedlings, comprising the following steps:

[0044] (1) Carry out virus-free tissue culture of sweet potato in a test tube, and the virus-free seedlings proliferate. Select the virus-free seedlings that have grown for 50 days, cut off the old roots, cut them into 4cm stem segments, and transplant them into sugar-free cultures equipped with vermiculite. In the box, each box is equipped with 670g of vermiculite, poured into 800mL of nutrient solution, transplanted 70 seedlings in each box, covered the box cover and sealed the gap between the box cover and the box body with parafilm; the nutrient solution contained 2g / L nitric acid Ammonium calcium and 170mg / LKH 2 PO 4 , and the rest is deionized water;

[0045] (2) After transplanting, put it on the culture rack in the airtight room, and use CO 2 The controller feeds CO into the culture box 2 , CO...

Embodiment 3

[0052] This embodiment provides a method for transplanting sweet potato virus-free seedlings for sugar-free cultivation of strong seedlings, comprising the following steps:

[0053] (1) Carry out virus-free tissue culture of sweet potato in a test tube, and the virus-free seedlings proliferate. Select the virus-free seedlings that have grown for 55 days, cut off the old roots, cut them into 4cm stem segments, and transplant them into sugar-free cultures equipped with vermiculite. In the box, each box is equipped with 670g of vermiculite, poured into 800mL of nutrient solution, transplanted 70 seedlings in each box, covered the box cover and sealed the gap between the box cover and the box body with parafilm; the nutrient solution contained 2g / L nitric acid Ammonium calcium and 170mg / LKH 2 PO 4 , and the rest is deionized water;

[0054] (2) After transplanting, put it on the culture rack in the airtight room, and use CO 2 The controller feeds CO into the culture box 2 , CO...

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Abstract

The invention belongs to the technical field of plant tissue culture, and particularly relates to a sugar-free culture and strong seedling transplanting method for virus-free sweet potato seedlings. The method includes: carrying out virus-free tissue culture on sweet potato in a test tube, propagating virus-free seedlings, cutting off old roots of the virus-free seedlings, cutting into stem segments, transplanting into sugar-free culture boxes filled with vermiculite, wherein each box is filled with 670 g of vermiculite, and irrigated with 800 mL of a nutrient solution, wherein the nutrient solution contains 2g / L calcium ammonium nitrate and 170mg / L KH2PO4, as well as the balance of deionized water; after 20 days of culture in the culture boxes, opening box covers, performing seedling exercising transition under natural conditions, and moving out to a greenhouse on the fourth day; taking out the tissue culture seedlings from the culture boxes and transplanting the tissue culture seedlings into greenhouse soil. According to the invention, the nutrition required by the growth of the tissue culture seedlings is satisfied, the procedure is simplified, and the production cost is saved.The permeability of matrix is maintained, the nutrition absorption and respiration of the root system of the tissue culture seedlings are facilitated, and the growth and rooting of the tissue cultureseedlings are best.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, in particular to a method for transplanting sweet potato virus-free seedlings for sugar-free culture and strong seedlings. Background technique [0002] Sweet potato has a variety of uses and is rich in nutrition. It is a functional food pursued by modern people. In recent years, the planting area of ​​sweet potato has steadily increased. However, due to the occurrence of viral diseases in sweet potato production areas, production has been reduced by 50-80%, or even no harvest. Production is in urgent need of a large amount of high-quality Detoxified healthy seedlings are used for industrial development. [0003] The traditional fast propagation of sweet potato in tissue culture is to use sugar as a carbon source for tissue culture seedlings to grow heterotrophically in a limited closed container. Due to the high relative humidity in the culture container, CO 2 The concentration is low, the p...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 解红娥王凌云解晓红李江辉吴宇浩王萌张鸿兴武宗信王立虎
Owner COTTON RES INST SHANXI ACAD OF AGRI SCI
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