A kind of wide-adapted plant tissue culture medium and 1/2 culture medium

Abstract

The invention discloses a wide-adaptability plant tissue culture medium and 1 / 2 culture medium, each 1000mL culture medium contains: KNO 3 2662mg, (NH 4 ) 2 SO 4 264mg, NH 4 h 2 PO 4 288mg, MgSO 4 ·7H 2 O 370mg, CaCl 2 332mg, MnSO 4 ·H 2 O 15mg, ZnSO 4 ·7H 2 O 4mg, H 3 BO 3 3mg, CoCl 2 ·6H 2 O 0.02mg, CuSO 4 ·5H 2 O 0.04mg, Na 2 MoO 4 2H 2 O 0.1mg, NaFeEDTA 37mg, inositol 100mg, niacin 2mg, thiamine hydrochloride 7mg, pyridoxine hydrochloride 1mg, glycine 2mg; halve the content of macroelements to obtain 1 / 2CS tissue culture medium. The plant tissue culture medium of the present invention can be widely used in various plant tissue cultures, the culture effect is equivalent to or better than MS medium, and it removes the existing There are explosive reagents NH contained in the culture medium 4 NO 3 , which is convenient for the purchase, storage and management of the medium; and the pH stability of the liquid medium prepared with it is good.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a plant tissue culture medium. Background technique [0002] MS culture is based on the design by Murashige and Skoog in 1962 and is currently the most widely used medium in plant tissue culture. According to the "Regulations on the Safety Management of Hazardous Chemicals" (Decree No. 591 of the State Council), the "Regulations on the Safety Management of Civilian Explosives" and the "List of Hazardous Chemicals Predisposing to Explosives" (2017 edition), the main components of MS medium are potassium nitrate, Ammonium nitrate is a controlled reagent for explosive precursors. As the national supervision becomes stricter, it becomes more and more difficult to purchase, store and use all explosive precursor reagents, especially ammonium nitrate with both nitrate nitrogen and ammonium nitrogen. Its pure product is prohibited from market circulation, which brings great...

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Problems solved by technology

According to the "Regulations on the Safety Management of Hazardous Chemicals" (Decree No. 591 of the State Council), the "Regulations on the Safety Management of Civilian Explosives" and the "List of Hazardous Chemicals Predisposing to Explosives" (2017 edition), the main components of MS medium are potassium nitrate, Ammonium nitrate is a controlled reagent for explosive precursors. As the national supervision becomes stricter, it becomes more and more difficult to purchase, store and use all explosive precursor reagents, especially ammonium nitrate with both nitrate nitrogen and ammonium nitrogen. Its pure product is banned from market circulation, which brings great difficulties to plant tissue culture

B5 culture is based on the design by Gamborg et al. for the cultivation of soybean root cells in 1968, and N6 culture is based on the design by Zhu Zhiqing et al. in 1974 for the cultivation of anthers of cereal crops such as rice. Both are derived from MS medium and are used in some plant tissues. It is widely used in culture research. Although the B5 and N6 mediums do not contain other explosive components except potassium nitrate, the contents of calcium ions and magnesium ions in both of them are greatly reduced, and the ammonium ions in the B5 medium are greatly reduced, and the N6 Potassium ions in the medium increase significantly, and large fluctuations in the concentration of these ions are detrimental to the tissue culture of some plants

CN102224802A Strawberry Proliferation Medium discloses a medium without ammonium nitrate, but its content of potassium nitrate and ammonium sulfate is too high, so it is only suitable for strawberry tissue culture; CN105104208A A kind of strawberry tissue culture medium and its preparation method discloses a medium without nitric acid Ammonium medium, which has achieved good results in strawberry tissue culture, but the content of urea and ammonium sulfate introduced in it is too high, which is unfavorable to the growth of various plants; CN108812313A is a basic medium for plant tissue culture and CN108812329A is a A medium for plant tissue culture without NH4NO3 respectively discloses a nitric acid-free medium, which obtains a good culture effect, but the newly introduced calcium nitrate and magnesium nitrate are both explosive reagents; CN109717076A A kind of plant tissue culture The special medium discloses a medium without ammonium nitrate, which can improve the success rate of plant tissue culture, but introduces the explosive reagent sodium nitrate, and it is only suitable for plant in vitro culture, and has no versatility

In addition, existing plant tissue culture media, including MS, B5, N6 and other disclosed plant tissue culture media, have large pH fluctuations when they are used to prepare liquid culture media, and all need to be adjusted when they are used for plant hydroponics pH, the process is tedious and time consuming

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