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Lactobacillus capable of producing high-yield gamma-aminobutyric acid as well as separation culture method and application of lactobacillus

An aminobutyric acid, isolation and culture technology, applied in microorganism-based methods, lactobacilli, applications, etc., can solve problems such as reducing the natural nutritional value of products, improve nutritional value and health care functions, excellent properties, and improve gastrointestinal tract. Effect

Pending Publication Date: 2020-02-04
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the development and research status of sweet potato beverages is mainly based on fermentation of raw sweet potatoes, sweet potato leaves and other compound fruits and vegetables. Most of its products have added a certain amount of color-protecting agents and stabilizers, which reduces the natural nutrition of the products. value

Method used

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  • Lactobacillus capable of producing high-yield gamma-aminobutyric acid as well as separation culture method and application of lactobacillus
  • Lactobacillus capable of producing high-yield gamma-aminobutyric acid as well as separation culture method and application of lactobacillus
  • Lactobacillus capable of producing high-yield gamma-aminobutyric acid as well as separation culture method and application of lactobacillus

Examples

Experimental program
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Effect test

Embodiment 1

[0066] The present embodiment provides a kind of lactobacillus high-yield gamma-aminobutyric acid, and the isolation method comprises the following steps:

[0067] (1) After diluting fermented fermented bean curd, a traditional fermented pickled product, with sterile physiological saline, spread it on bromocresol purple-MRS medium plate, and culture it at a constant temperature of 37°C for 48 hours, and screen out the bromcresol purple- Colonies that turn yellow on the MRS plate;

[0068] (2) Pick the bacterium colonies that can make the bromocresol purple-MRS flat plate turn yellow that step (1) obtains, repeat streaking culture 3 times, obtain pure species bacterium colony, carry out after Gram staining then, microscopic examination and carry out Catalase test, screen out strains with Gram staining positive and catalase test negative, and store them for later use;

[0069] (3) The strains screened in step (2) are subjected to primary screening for producing gamma-aminobutyr...

Embodiment 2

[0072] The present embodiment provides a kind of lactobacillus high-yield gamma-aminobutyric acid, and the isolation method comprises the following steps:

[0073] (1) After diluting fermented radish, a traditional fermented pickled product, with sterile physiological saline, spread it on a bromcresol purple-MRS medium plate, and culture it at a constant temperature of 37°C for 72 hours to screen out bromcresol purple-MRS Colonies that turn yellow on the plate;

[0074] (2) Pick the bacterium colonies that can make the bromocresol purple-MRS flat plate turn yellow that step (1) obtains, repeat streaking culture 3 times, obtain pure species bacterium colony, carry out after Gram staining then, microscopic examination and carry out Catalase test, screen out strains with Gram staining positive and catalase test negative, and store them for later use;

[0075] (3) The strains screened in step (2) are subjected to primary screening for producing gamma-aminobutyric acid, and the st...

Embodiment 3

[0078] The lactobacillus screened in embodiment 1 is carried out the observation of bacterium colony form and individual form, the result is as follows figure 1 and figure 2 as shown ( figure 1 It is the colony form figure of the lactobacillus with high production of γ-aminobutyric acid of the present invention, figure 2 It is the individual morphology diagram of the lactobacillus with high γ-aminobutyric acid production in the present invention). Depend on figure 1 It can be seen that the colony of lactobacillus with high yield of gamma-aminobutyric acid of the present invention is small, the center of the colony is raised, the surface is smooth and moist, the edges are neat and complete, and acid is produced. Depend on figure 2 It can be seen that under the optical microscope, the individual shape of the lactobacillus with high yield of gamma-aminobutyric acid of the present invention is rod-shaped, and there are single, paired and chain-like existences, and these cha...

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Abstract

The invention relates to lactobacillus capable of producing high-yield gamma-aminobutyric acid as well as a separation culture method and application of the lactobacillus. The lactobacillus capable ofproducing high-yield gamma-aminobutyric acid is named as lactobacillus plantarum D-9 strain, wherein the preservation unit is China General Microbiological Culture Collection Center (CGMCC), the preservation time is December 4, 2018, the preservation number is CGMCC No.16864, and the address is 3#, 1# Courtyard, Beichen West Road, Chaoyang District, Beijing. The lactobacillus has excellent characteristic of producing high-yield gamma-aminobutyric acid; and the lactobacillus has important significance in increasing the nutritional value and the health-care function of food when being applied to fermented food (fermented beverage).

Description

technical field [0001] The invention belongs to the technical field of food fermentation, and in particular relates to a lactobacillus and its isolation and cultivation method and application, in particular to a lactobacillus with high γ-aminobutyric acid production and its isolation and cultivation method and application. Background technique [0002] Sweet potato is an important food crop after rice, wheat and corn in my country, and it is a healthy food with both medicine and food. Sweet potatoes are rich in dietary fiber, carotene, vitamins A, B, C, E and more than 10 kinds of trace elements such as potassium, iron, copper, selenium and calcium. They have high nutritional value and are called the most nutritious by nutritionists. Balanced natural health food. Modern medical research has found that sweet potatoes contain a variety of functional factors, which can prevent cardiovascular diseases, prevent constipation and intestinal diseases, eliminate oxygen free radicals...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A23L2/38A23L33/00C12R1/25
CPCC12N1/20C12N1/02A23L2/382A23L33/00A23V2002/00C12R2001/25C12N1/205A23V2400/123A23V2400/249A23V2200/30A23V2200/32A23V2200/31A23V2200/322A23V2200/308
Inventor 耿伟涛王进杨曼王金菊王艳萍
Owner TIANJIN UNIV OF SCI & TECH
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