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A kind of aspergillus flavus control composition containing perillaldehyde and calcium ion chelating agent edta and its application

A technology of calcium ion chelating agent and perilla aldehyde, which can be applied in application, edible seed preservation, bactericide, etc., can solve the effect of fungal mycelium growth and sporulation. There are few studies on the effect of EDTA on perilla aldehyde bacteriostasis. It can achieve the effect of significant prevention and treatment, promoting autophagy and inhibiting growth.

Active Publication Date: 2021-06-15
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a commonly used calcium ion chelating agent, EDTA has little research on its effect on inhibiting fungal mycelial growth and sporulation.
At present, there is no research on the promoting effect of EDTA on the antibacterial effect of perillaldehyde at home and abroad.

Method used

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  • A kind of aspergillus flavus control composition containing perillaldehyde and calcium ion chelating agent edta and its application
  • A kind of aspergillus flavus control composition containing perillaldehyde and calcium ion chelating agent edta and its application
  • A kind of aspergillus flavus control composition containing perillaldehyde and calcium ion chelating agent edta and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A flavus control composition containing perillaldehyde and calcium ion chelating agent EDTA, comprising A component and B component, the A component is perillaldehyde (Shanghai Bangcheng Chemical Industry, CAS#: 2111-75- 3), the B component is calcium ion chelating agent EDTA; wherein the concentration of the A component is 100µg / L, and the concentration of the B component is 200µg / L.

[0021] The preparation method of the above-mentioned Aspergillus flavus control composition containing perillaldehyde and calcium ion chelating agent EDTA comprises the following steps: weighing the corresponding weight component raw materials according to the concentration requirements of each component in the composition, and the solid raw material of the A component Dissolve perillaldehyde in 1 times the volume of absolute ethanol first, then use sterile water to dissolve it completely, then mix it with component B calcium ion chelating agent EDTA, and make the composition of perillald...

Embodiment 2

[0023] The composition in Example 1 was selected to detect its inhibitory effect on Aspergillus flavus. On the ultra-clean workbench, first inoculate the Aspergillus flavus isolated from Fujian onto the PDA solid medium. After 48-72 hours of dark cultivation at a temperature of 29°C, inoculate the cultured Aspergillus flavus 5 mm into 5 In different media, at 29°C, after 4-5 days of dark cultivation, the growth rate of pathogenic bacteria was counted by the cross method.

[0024] 5 different media are

[0025] Control group (CK): PDA solid medium;

[0026] Treatment group 1 (PAE): PDA solid medium + 100µg / L PAE;

[0027] Treatment group 2 (EDTA): PDA solid medium + 200µg / L EDTA;

[0028] Treatment group 3 (PAE + EDTA): PDA solid medium + 100µg / L PAE + 200µg / L EDTA

[0029] Treatment group 4 (PAE + Ca 2+ ): PDA solid medium + 100µg / L PAE + 1.11g / LCaCl 2 .

[0030] The statistics of growth rate found that the medium containing 100µg / L PAE, 200µg / L EDTA, and 100µg / L PAE+20...

Embodiment 3

[0032] The composition in Example 1 was selected to detect its inhibitory effect on the germination of Aspergillus flavus spores and the concentration of intracellular calcium ions. On the ultra-clean workbench, first inoculate the isolated Aspergillus flavus from Fujian on the PDA solid medium, and culture it in the dark at 29°C for 5-7 days, then collect the spores to prepare 1×10 6 Aspergillus flavus spore suspension per mL, take 2 μL spore suspension and inoculate to final concentrations of 100 µg / L PAE, 200 µg / L EDTA, 100 µg / L PAE+200 µg / L EDTA and 100 µg / L PAEPAE+1.11g / LCaCl 2 The germination rate was counted and photographed after culturing in the dark at 29°C for 5-6 hours on a PDA plate, and water treatment was used as a control. According to the statistics of germination rate, 100µg / L PAE, 200µg / L EDTA, 100µg / L PAE+200µg / L EDTA can all inhibit germination, but 100µg / L PAE+1.11g / LCaCl 2 The treatment had no inhibitory effect on spore germination ( figure 2 A). T...

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Abstract

The invention provides a composition for controlling aspergillus flavus containing perillaldehyde and calcium ion chelating agent EDTA and application thereof. The composition comprises A component and B component, component A is perillaldehyde, component B is calcium ion chelating agent EDTA, the concentration of perillaldehyde is 100µg / L-1000µg / L, the calcium ion chelating agent EDTA The concentration is 200µg / L‑1000µg / mL. The composition of the invention has a strong effect of inhibiting the peanut aflatoxin and can be used for preparing medicines for preventing and treating the peanut aflatoxin. The perilla aldehyde of the present invention is derived from natural sources, has no toxic and side effects, is not easy to cause drug resistance of pathogenic microorganisms, and can be used to prevent and treat peanut aflatoxin; it is safe for humans and animals, and is a low-toxic, effective antibacterial biological pesticide . The use of perillaldehyde and calcium ion chelating agent EDTA as a fungicide for peanut Aspergillus flavus has great potential.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a peanut aflatoxin control composition containing perillaldehyde and a calcium ion chelating agent EDTA and an application thereof. Background technique [0002] Aspergillus flavus is a pathogen of plants, animals and humans, causing losses in the tens of billions of dollars each year. More than 1 / 4 of the crops in the world are polluted by Aspergillus flavus every year. During the storage and transportation of grains after harvest, Aspergillus flavus strains can infect grains and produce a large amount of carcinogenic and acute toxic compounds - aflatoxins. Under normal circumstances, peanuts and their products in oil crops have the most chance of being contaminated. For many years, the prevention and control of Aspergillus flavus has been mainly based on chemical control. However, most of the chemical pesticides are non-naturally occurring substances, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N35/02A01N37/44A01P3/00A23B9/26
CPCA01N35/02A01N37/44A23B9/26
Inventor 刘裴清陈庆河李本金王荣波翁启勇
Owner INST OF PLANT PROTECTION FAAS
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