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Application of PLEKHG5 gene methylation in asthenospermia diagnostic agent and kit

A diagnostic kit and methylation technology, applied in the determination/examination of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of less methylated regions and accurate diagnosis of subjects, etc.

Pending Publication Date: 2020-01-21
深圳市龙华区人民医院
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Problems solved by technology

[0004] However, there are very few reported methylated regions that can be used as molecular markers of asthenospermia, and it is difficult to accurately diagnose whether the subject has asthenospermia. Therefore, it is necessary to identify more methylated regions related to asthenospermia. basement region, thus providing more basis for the application of subsequent diagnosis and treatment

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  • Application of PLEKHG5 gene methylation in asthenospermia diagnostic agent and kit
  • Application of PLEKHG5 gene methylation in asthenospermia diagnostic agent and kit
  • Application of PLEKHG5 gene methylation in asthenospermia diagnostic agent and kit

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[0015] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0016] 1. Sperm separation

[0017] Seven asthenospermia volunteers (group A) and eight healthy volunteers (group N) were recruited, and sperm samples were obtained from the volunteers for genome-wide sequencing of methylation levels. Computer-assisted sperm analysis (CASA) was performed using a Sperm Class Analyzer, and sperm morphology was assessed by Diff-Quik rapid staining. Sperm cells with different vigor were separated by the following method: centrifuged at 300×g for 20 min to prepare discontinuous density gradient fractions (divided into 47.5, 57, 76 and 95% concentration layers), and from the 95% concentration layer (fraction a) High motility sperm cells were collected, and low motility sperm cells were c...

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Abstract

The invention relates to application of the methylation level of a human PLEKHG5 gene promoter region in preparation of a detection agent for detecting sperm motility. The human PLEKHG5 gene promoterregion is located in a region corresponding to 6545514-6548214 sites of human No.1 chromosome. Analysis and study based on whole-genome methylation sequencing results and verification find that whether part of methylation sites of the human PLEKHG5 gene promoter region are methylated has significance difference between asthenospermia patients and healthy people, which can be used as a molecular marker to diagnose whether subjects suffer from asthenospermia. When all methylation sites of the region are not methylated, the possibility that subjects suffer from the asthenospermia is larger. The molecular marker can provide auxiliary evaluation information for diagnosis of the asthenospermia and identify whether the subjects suffer from the asthenospermia with other diagnosis indexes.

Description

technical field [0001] The invention relates to the field of human reproductive health, more particularly, relates to the application of the methylation level of the promoter region of human PLEKHG5 gene in the preparation of diagnostic agents for asthenospermia, and a diagnostic kit for asthenospermia. Background technique [0002] More than 40% of infertile men were detected to suffer from asthenospermia. The etiology of asthenospermia is multifactorial, for example, it may be closely related to endocrine disorders, environmental factors, life experiences and even aging. Gene knockout and mutation studies in mice have shown that mutations in some genes are associated with altered sperm motility, but none of these genes have been shown to be associated with sperm motility in human studies. Although nearly 50% of infertility cases are believed to be caused by genetic defects, in some cases of male infertility, genetic factors do not appear to be the main factor. Accumulati...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/154
Inventor 杜野薛兴奎古艳丽陈晓玲马晓瑞
Owner 深圳市龙华区人民医院
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