A kind of immobilized bacterial agent and its preparation method and application

A technology of immobilized carriers and bacterial agents, applied in biochemical equipment and methods, chemical instruments and methods, fixed on/in organic carriers, etc., can solve the complicated process, reduce the activity of bacteria, and the cost of biological denitrification Advanced problems, to achieve the effects of obvious porous characteristics, increased speed, and easy application

Active Publication Date: 2021-03-30
POWERCHINA WATER ENVIRONMENT GOVERANCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the existing problems of high cost of biological denitrification, cumbersome process, and immobilized process reducing the activity of bacteria, the invention provides an immobilized bacterial agent

Method used

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  • A kind of immobilized bacterial agent and its preparation method and application
  • A kind of immobilized bacterial agent and its preparation method and application
  • A kind of immobilized bacterial agent and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0046] The embodiment of the present invention provides an immobilized bacterial agent, comprising Acinetobacter and glutinous rice bran. The Acinetobacter is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the preservation number of the Acinetobacter is CGMCCNO. 16620; the water absorption rate of the bran is 40%.

[0047] The mass ratio of the rice bran to the bacterial suspension of the Acinetobacter is 5:2, and the Acinetobacter in the bacterial suspension is in the logarithmic growth phase; the OD600 of the bacterial suspension is 0.613.

[0048] The preparation method of the above-mentioned immobilized bacterial agent comprises the following steps:

[0049] Step a, culturing the bacterial suspension of the Acinetobacter to the logarithmic growth phase;

[0050] Step b. Sterilize the bran at 121°C for 30 minutes, then mix it with the bacterial suspension under aseptic conditions at a speed of 200 rpm ...

Embodiment 2

[0052] The embodiment of the present invention provides an immobilized bacterial agent, comprising Acinetobacter and glutinous rice bran. The Acinetobacter is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the preservation number of the Acinetobacter is CGMCCNO. 16620; the water absorption rate of the bran is 45%.

[0053] The mass ratio of the rice bran to the bacterial suspension of the Acinetobacter is 4.5:2.5, and the Acinetobacter in the bacterial suspension is in the logarithmic growth phase; the OD600 of the bacterial suspension is 0.63.

[0054] The preparation method of the above-mentioned immobilized bacterial agent comprises the following steps:

[0055] Step a, culturing the bacterial suspension of the Acinetobacter to the logarithmic growth phase;

[0056] Step b. Sterilize the bran at 125°C for 25 minutes, and then mix it with the bacterial suspension at a speed of 300 rpm for 8 minutes unde...

Embodiment 3

[0058] The embodiment of the present invention provides an immobilized bacterial agent, comprising Acinetobacter and glutinous rice bran. The Acinetobacter is preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the preservation number of the Acinetobacter is CGMCCNO. 16620; the water absorption rate of the bran is 35%.

[0059] The mass ratio of the rice bran to the bacterial suspension of the Acinetobacter is 5.5:1.5, and the Acinetobacter in the bacterial suspension is in the logarithmic growth phase; the OD600 of the bacterial suspension is 0.605.

[0060] The preparation method of the above-mentioned immobilized bacterial agent comprises the following steps:

[0061] Step a, culturing the bacterial suspension of the Acinetobacter to the logarithmic growth phase;

[0062] Step b. Sterilize the bran at 120°C for 35 minutes, then mix it with the bacterial suspension under aseptic conditions at a speed of 100 ...

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Abstract

The invention relates to the technical field of microbial agent immobilization, and particularly discloses an immobilized microbial agent and a preparation method and application thereof. The immobilized microbial agent comprises acinetobacter and an immobilized carrier, wherein the immobilized carrier is rice bran. The preparation method of the immobilized microbial agent at least comprises the following steps: culturing the acinetobacter to obtain bacterial suspension; and carrying out a sterilization treatment on the rice bran, uniformly mixing the rice bran and the bacterial suspension under the aseptic condition, and drying the mixture to obtain the immobilized microbial agent. According to the invention, through preparing the immobilized microbial agent, the acinetobacter is loaded into the immobilized carrier, thus ensuring that the physiological activity and stability of microorganism are not affected and providing aerobic and anoxic micro-environments for the microorganism, thereby being beneficial to the growth of the microbial agent and the performance of nitrogen removal by denitrification of the microbial agent.

Description

technical field [0001] The invention relates to the technical field of bacterial agent immobilization, in particular to an immobilized bacterial agent and its preparation method and application. Background technique [0002] The rapid development of industry and agriculture and human production activities have brought serious pollution to the water environment. Excessive nitrogen is the most common type of pollution in water pollution. It will cause eutrophication in water, cause algae to multiply, reduce dissolved oxygen in water, and make water black and smelly. This not only endangers human water safety, but also seriously affects water quality. Ecological balance; In addition, excessive nitrogen discharge will also lead to the destruction of nitrogen balance, thus causing the water body to lose its self-purification function. Therefore, nitrogen-rich sewage needs to be denitrified before it can be discharged into the natural environment. [0003] Biological denitrifica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/12C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/16C12N11/12
Inventor 陈惠明蒋自胜翟德勤余艳鸽郑茂盛商放泽俞静雯
Owner POWERCHINA WATER ENVIRONMENT GOVERANCE
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