A kind of immobilized bacterial agent and its preparation method and application

A technology for immobilizing carriers and inoculants, applied in biochemical equipment and methods, immobilized enzymes, chemical instruments and methods, etc., can solve the problems of cumbersome process, high cost of biological denitrification, and reducing the activity of bacteria, and achieves a high level of improvement. Biological activity, improvement of spheroidization and mechanical strength, reduction of biological toxicity

Active Publication Date: 2021-03-30
POWERCHINA WATER ENVIRONMENT GOVERANCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the existing problems of high cost of biological denitrification, cumbersome process, and immobilized process reducing the activity of bacteria, the invention provides an immobilized bacterial agent

Method used

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  • A kind of immobilized bacterial agent and its preparation method and application

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Embodiment 1

[0052] The embodiment of the present invention provides an immobilized bacterial agent, comprising Acinetobacter and an immobilized carrier. The Acinetobacter is preserved in the General Microbiology Center of the China Committee for the Collection of Microorganisms, and the preservation number of the Acinetobacter is CGMCC NO.16620.

[0053] The preparation method of the above-mentioned immobilized bacterial agent comprises the following steps:

[0054] Step a, adding 8 g of polyvinyl alcohol into 100 mL of distilled water, and heating at 90° C. for 60 min to obtain the first solution with a concentration of 8 wt %;

[0055] Step b. Add 1 g of the sodium alginate to 100 mL of distilled water, heat at 80° C. for 20 min to obtain a second solution with a concentration of 1 wt %, and mix the second solution with the first solution evenly , sterilized at 121°C for 20 minutes to obtain the third solution;

[0056] Step c, adding 2 g of sterilized activated carbon into 10 mL of t...

Embodiment 2

[0059] The embodiment of the present invention provides an immobilized bacterial agent, comprising Acinetobacter and an immobilized carrier. The Acinetobacter is preserved in the General Microbiology Center of the China Committee for the Collection of Microorganisms, and the preservation number of the Acinetobacter is CGMCC NO.16620.

[0060] The preparation method of the above-mentioned immobilized bacterial agent comprises the following steps:

[0061] Step a, adding 10 g of the polyvinyl alcohol into 100 mL of distilled water and heating at 95° C. for 50 min to obtain the first solution with a concentration of 10 wt %;

[0062] Step b. Add 2 g of the sodium alginate into 100 mL of distilled water, heat at 85° C. for 15 min to obtain a second solution with a concentration of 2 wt %, and mix the second solution with the first solution evenly , sterilized at 125°C for 15 minutes to obtain a third solution;

[0063] Step c. Add 1.7 g of sterilized activated carbon into 10 mL ...

Embodiment 3

[0066] The embodiment of the present invention provides an immobilized bacterial agent, comprising Acinetobacter and an immobilized carrier. The Acinetobacter is preserved in the General Microbiology Center of the China Committee for the Collection of Microorganisms, and the preservation number of the Acinetobacter is CGMCC NO.16620.

[0067] The preparation method of the above-mentioned immobilized bacterial agent comprises the following steps:

[0068] Step a, adding 9 g of the polyvinyl alcohol into 100 mL of distilled water and heating at 85° C. for 70 min to obtain the first solution with a concentration of 9 wt %;

[0069] Step b. Add 1.5 g of the agar to 100 mL of distilled water, heat at 75° C. for 25 min to obtain a second solution with a concentration of 1.5 wt %, and mix the second solution with the first solution evenly , sterilized at 120°C for 25 minutes to obtain a third solution;

[0070] Step c. Add 1.8 g of sterilized activated carbon into 10 mL of the bact...

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Abstract

The invention relates to the technical field of bacterial agent immobilization, and specifically discloses an immobilized bacterial agent and a preparation method and application thereof. The immobilized bacterial agent comprises Acinetobacter and an immobilized carrier. The preparation method of the immobilized bacterial agent at least includes the following steps: dissolving the polyvinyl alcohol in distilled water to obtain a first solution; dissolving the natural polymer gel carrier in distilled water to obtain a second solution , and the second solution is mixed with the first solution and sterilized to obtain a third solution; the sterilized activated carbon is added to the bacterial suspension, left for adsorption, and the adsorbed bacterial suspension is added to the In the third solution, mix uniformly to obtain the fourth solution; add the fourth solution dropwise to CaCl 2 In a saturated boric acid solution, react under the condition of 3-5°C, filter, wash and dry to obtain the immobilized bacterial agent. The invention prepares the immobilized bacterial agent by embedding method, the method is simple, the condition is mild, and the stability is good.

Description

technical field [0001] The invention relates to the technical field of bacterial agent immobilization, in particular to an immobilized bacterial agent and its preparation method and application. Background technique [0002] The rapid development of industry and agriculture and human production activities have brought serious pollution to the water environment. Excessive nitrogen is the most common type of pollution in water pollution. It will cause eutrophication in water, cause algae to multiply, reduce dissolved oxygen in water, and make water black and smelly. This not only endangers human water safety, but also seriously affects water quality. Ecological balance; In addition, excessive nitrogen discharge will also lead to the destruction of nitrogen balance, thus causing the water body to lose its self-purification function. Therefore, nitrogen-rich sewage needs to be denitrified before it can be discharged into the natural environment. [0003] Biological denitrifica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/084C12N11/10C12N11/04C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/16C12N11/04C12N11/08C12N11/10C12N1/205C12R2001/01
Inventor 陈惠明蒋自胜翟德勤余艳鸽郑茂盛李金波孙小玲
Owner POWERCHINA WATER ENVIRONMENT GOVERANCE
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