Immobilized microbial agent, and preparation method and application thereof

A technology of immobilized carriers and bacterial agents, applied in biochemical equipment and methods, chemical instruments and methods, fixed on/in organic carriers, etc., can solve the problems of high cost of biological denitrification, reduction of bacterial activity, process cumbersome and other problems, to achieve the effect of improving spheroidization and mechanical strength, improving biological activity and reducing biological toxicity

Active Publication Date: 2020-01-17
POWERCHINA WATER ENVIRONMENT GOVERANCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the existing problems of high cost of biological denitrification, cumbersome process, and

Method used

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  • Immobilized microbial agent, and preparation method and application thereof

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Effect test

Embodiment 1

[0052] The embodiment of the present invention provides an immobilized bacterial agent, comprising Acinetobacter and an immobilized carrier. The Acinetobacter is preserved in the General Microbiology Center of the China Committee for the Collection of Microorganisms, and the preservation number of the Acinetobacter is CGMCC NO.16620.

[0053] The preparation method of the above-mentioned immobilized bacterial agent comprises the following steps:

[0054] Step a, adding 8 g of polyvinyl alcohol into 100 mL of distilled water, and heating at 90° C. for 60 min to obtain the first solution with a concentration of 8 wt %;

[0055] Step b. Add 1 g of the sodium alginate to 100 mL of distilled water, heat at 80° C. for 20 min to obtain a second solution with a concentration of 1 wt %, and mix the second solution with the first solution evenly , sterilized at 121°C for 20 minutes to obtain the third solution;

[0056] Step c, adding 2 g of sterilized activated carbon into 10 mL of t...

Embodiment 2

[0059] The embodiment of the present invention provides an immobilized bacterial agent, comprising Acinetobacter and an immobilized carrier. The Acinetobacter is preserved in the General Microbiology Center of the China Committee for the Collection of Microorganisms, and the preservation number of the Acinetobacter is CGMCC NO.16620.

[0060] The preparation method of the above-mentioned immobilized bacterial agent comprises the following steps:

[0061] Step a, adding 10 g of the polyvinyl alcohol into 100 mL of distilled water and heating at 95° C. for 50 min to obtain the first solution with a concentration of 10 wt %;

[0062] Step b. Add 2 g of the sodium alginate into 100 mL of distilled water, heat at 85° C. for 15 min to obtain a second solution with a concentration of 2 wt %, and mix the second solution with the first solution evenly , sterilized at 125°C for 15 minutes to obtain a third solution;

[0063] Step c. Add 1.7 g of sterilized activated carbon into 10 mL ...

Embodiment 3

[0066] The embodiment of the present invention provides an immobilized bacterial agent, comprising Acinetobacter and an immobilized carrier. The Acinetobacter is preserved in the General Microbiology Center of the China Committee for the Collection of Microorganisms, and the preservation number of the Acinetobacter is CGMCC NO.16620.

[0067] The preparation method of the above-mentioned immobilized bacterial agent comprises the following steps:

[0068] Step a, adding 9 g of the polyvinyl alcohol into 100 mL of distilled water and heating at 85° C. for 70 min to obtain the first solution with a concentration of 9 wt %;

[0069] Step b. Add 1.5 g of the agar into 100 mL of distilled water, heat at 75° C. for 25 min to obtain a second solution with a concentration of 1.5 wt %, and mix the second solution with the first solution evenly , sterilized at 120°C for 25 minutes to obtain a third solution;

[0070] Step c. Add 1.8 g of sterilized activated carbon into 10 mL of the ba...

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Abstract

The invention relates to the technical field of microbial agent immobilization, and specifically discloses an immobilized microbial agent as well as a preparation method and application thereof. The immobilized microbial agent contains acinetobacter and an immobilization carrier. The preparation method of the immobilized microbial agent at least comprises the following steps: dissolving polyvinylalcohol in distilled water so as to obtain a first solution; dissolving a natural polymeric gel carrier in distilled water so as to obtain a second solution; mixing the second solution with the firstsolution, and carrying out sterilization so as to obtain a third solution; adding sterilized activated carbon into a bacterial suspension, carrying out static adsorption, adding the bacterial suspension after adsorption into the third solution, and carrying out uniform mixing so as to obtain a fourth solution; and then, adding dropwise the fourth solution into a saturated boric acid solution of CaCl2, carrying out areaction at 3-5 DEG C, and carrying out filtering, washing and drying so as to obtain the immobilized microbial agent. According to the preparation method of the immobilized microbial agent, the immobilized microbial agent is prepared by adopting an embedding way; and thus, the preparation method is simpleand convenient, mild in conditions and good in stability.

Description

technical field [0001] The invention relates to the technical field of bacterial agent immobilization, in particular to an immobilized bacterial agent and its preparation method and application. Background technique [0002] The rapid development of industry and agriculture and human production activities have brought serious pollution to the water environment. Excessive nitrogen is the most common type of pollution in water pollution. It will cause eutrophication in water, cause algae to multiply, reduce dissolved oxygen in water, and make water black and smelly. This not only endangers human water safety, but also seriously affects water quality. Ecological balance; In addition, excessive nitrogen discharge will also lead to the destruction of nitrogen balance, thus causing the water body to lose its self-purification function. Therefore, nitrogen-rich sewage needs to be denitrified before it can be discharged into the natural environment. [0003] Biological denitrifica...

Claims

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Application Information

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IPC IPC(8): C12N11/08C12N11/10C12N11/04C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/16C12N11/04C12N11/08C12N11/10C12N1/205C12R2001/01
Inventor 陈惠明蒋自胜翟德勤余艳鸽郑茂盛李金波孙小玲
Owner POWERCHINA WATER ENVIRONMENT GOVERANCE
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