Preparation method of emblic leafflower fruit polysaccharide

A technology of emblica and polysaccharides, which is applied in the field of preparation of polysaccharides from emblica, can solve the problems of high safety risks, complicated operation steps, and not widely used, and achieve the effect of easy promotion

Inactive Publication Date: 2020-01-17
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic research on emblica polysaccharides is less, and it is still in its infancy. CN104628876A, published on May 20, 2015, discloses an extraction method of emblica polysaccharides. The process includes ultrasonic extraction, and the extraction method is It is not widely used in production, and the patent uses toxic reagents such as acetone, ether, n-butanol, and chloroform in the sample processing process. The production process has a large safety risk, serious environmental pollution, and the quality of the sample cannot be guaranteed. The operation steps are complicated, which is not conducive to the promotion of industrialization

Method used

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  • Preparation method of emblic leafflower fruit polysaccharide
  • Preparation method of emblic leafflower fruit polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Take 10 g of emblica medicinal material with a particle size of 5 mesh, and extract it twice with 20 g of 90% ethanol at a temperature of 45 ° C, 5 hours each time, evaporate the solvent to obtain the medicinal residue, add 10 BV of distilled water to the medicinal residue, and extract 2 times at 80 ° C. Each time, 50 minutes each time, the water extract was concentrated to a liquid density of 0.5g / ml, and 90% ethanol was added until the alcohol concentration was 78%, and it was left to stand for 5 hours, centrifuged (3000 rpm, time 25 minutes), and the obtained precipitate was anhydrous Washed repeatedly with ethanol, spray dried to obtain 355.11 mg of solid.

Embodiment 2

[0043] Take 10g of emblica medicinal material with a particle size of 10 mesh, extract 3 times with 30g of 75% ethanol at a temperature of 55°C, each time for 3h, evaporate the solvent to obtain the medicinal residue, add 16BV distilled water to the medicinal residue, and extract 4 at 85°C Once, each time for 80 minutes, the water extract was concentrated to a liquid density of 2 g / ml, and 98% ethanol was added to reach an alcohol concentration of 82%, left to stand for 15 hours, centrifuged (3000 rpm, time 20 minutes), and the obtained precipitate was washed with absolute ethanol After repeated washing and spray drying, 354.21 mg of solid was obtained.

Embodiment 3

[0045] Take 10g of emblica medicinal material with a particle size of 20 mesh, extract 4 times with 10g of 70% ethanol at a temperature of 60°C, each time for 1h, evaporate the solvent to obtain medicinal residues, add 12BV of distilled water to the medicinal residues, and extract at 90°C for 3 Each time, 60 minutes each time, the water extract was concentrated to a liquid density of 1 g / ml, and 95% ethanol was added until the alcohol concentration was 80%, left to stand for 10 hours, centrifuged (3000 rpm, time 30 minutes), and the obtained precipitate was washed with absolute ethanol After repeated washing and drying under reduced pressure, 366.23 mg of solid was obtained.

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Abstract

The invention relates to a preparation method of emblic leafflower fruit polysaccharide, which comprises the following steps: (1) carrying out warm-soaking extraction on emblic leafflower fruit medicinal material, and volatilizing a solvent to obtain medicinal residues; and (2) adding distilled water into the medicinal residues, extracting at 80-100 DEG C, concentrating an obtained water extract,adding 90-98% ethanol until the alcohol concentration is 70-90%, standing, centrifuging or filtering, repeatedly washing an obtained precipitate with absolute ethyl alcohol, and drying. The method issimple to operate and beneficial to industrial popularization.

Description

technical field [0001] The invention relates to a method for preparing active ingredients of traditional Chinese medicines, in particular to a method for preparing polysaccharides from Amla emblica. Background technique [0002] Amla emblica Phyllanthus emblica The fruit of L. has the effects of resolving phlegm, promoting body fluid, relieving cough and detoxification. It is mainly used to treat cold and fever, cough and sore throat, diphtheria, restlessness and dry mouth. Distributed in Fujian, Guangdong, Guangxi, Yunnan, Guizhou, Sichuan, Taiwan and other places. Most of emblica are wild or semi-wild, almost free from industrial pollution, and are veritable raw materials for green food processing. [0003] Amla polysaccharide has antioxidant and anti-tumor effects, can significantly reduce the content of lipid peroxide (LPO) in the liver of mice, and significantly increase the activity of superoxide dismutase (SOD) and the content of glutathione (GSH) in whole blood. ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K36/47
CPCA61K36/47A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/53C08B37/0003
Inventor 张兰珍张鸿雁梁文仪张秋楠常子豪
Owner BEIJING UNIV OF CHINESE MEDICINE
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